pro melanin, Melanin as a target for melanoma chemotherapy: pro-oxidant effect of oxygen and metals on melanoma viability.

Melanin as a target for melanoma chemotherapy: pro-oxidant effect of oxygen and metals on melanoma viability.

Melanoma cells have a poor means to mediate oxidative stress, which can be attributed to constitutive abnormalities of their melanosomes. We hypothesize that disorganization of the melanosomes will permit chemical concentrating on of the melanin inside. Chemical research present that beneath oxidative circumstances, artificial melanins display elevated metallic affinity and a susceptibility to redox biking with oxygen to kind reactive oxygen species.
The electron paramagnetic resonance (EPR)-active 5,5′-dimethyl-pyrollidine N-oxide spin adduct was used to point out that binding of divalent Zn or Cu to melanin induces a pro-oxidant response beneath oxygen, producing superoxide and hydroxyl radicals. An analogous pro-oxidant behaviour is seen in melanoma cell strains beneath exterior peroxide stress.
Melanoma cultures grown beneath 95% O2/5% CO2 atmospheres present markedly lowered viability as in contrast with regular melanocytes. Cu- and Zn-dithiocarbamate complexes, which induce passive uptake of the metallic ions into cells, present important antimelanoma exercise. The antimelanoma impact of metal- and oxygen-induced stress seems additive slightly than synergistic; each therapies are proven to be considerably much less poisonous to melanocytes.

Human Th2 cells selectively specific the orexigenic peptide, pro-melanin-concentrating hormone.

Th1 and Th2 cells characterize the 2 predominant practical subsets of CD4(+) T helper cell, and are outlined by their cytokine expression. Human Th1 cells specific IFNgamma, while Th2 cells specific IL-4, IL-5, and IL-13. Th1 and Th2 cells have distinct immunological capabilities, and might drive completely different immunopathologies.
Right here, we present that in vitro-differentiated human Th2 cells extremely selectively specific the gene for pro-melanin-concentrating hormone (PMCH), utilizing real-time RT-PCR, enzyme immunoassay, and Western blot evaluation. PMCH encodes the prohormone, promelanin-concentrating hormone (PMCH), which is proteolytically processed to provide a number of peptides, together with the orexigenic hormone melanin-concentrating hormone (MCH).
PMCH expression by Th2 cells was activation responsive and elevated all through the 28-day differentiation in parallel with the expression of the Th2 cytokine genes. MCH immunoreactivity was detected within the differentiated Th2 however not Th1 cell tradition supernatants after activation, and contained the whole PMCH protein, along with a number of smaller peptides.
Human Th1 and Th2 cells had been remoted by their expression of IFNgamma and CRTH2, respectively, and the ex vivo Th2 cells expressed PMCH upon activation, in distinction to the Th1 cells. As a result of Th2 cells are central to the pathogenesis of allergic illnesses together with bronchial asthma, expression of PMCH by activated Th2 cells in vivo might straight hyperlink allergic irritation to vitality homeostasis and will contribute to the affiliation between bronchial asthma and weight problems.
pro melanin, Melanin as a target for melanoma chemotherapy: pro-oxidant effect of oxygen and metals on melanoma viability.

Extracellular Vesicles from Korean Codium fragile and Sargassum fusiforme Negatively Regulate Melanin Synthesis

Though varied marine substances have been exploited for the event of beauty merchandise, no earlier research has examined the potential of seaweed extracellular vesicles (EV) in such functions. Our outcomes revealed that EV from Codium fragile and Sargassum fusiforme successfully decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, related to downregulation of MITF (microphthalmia-associated transcription issue), tyrosinase and TRP1 (tyrosinase-related proteins 1).
The simplest inhibitory concentrations of EV had been 250 μg/ml for S. fusiforme and 25 μg/ml for C. fragile, with out affecting the viability of MNT-1 cells. Each EV lowered melanin synthesis within the epidermal basal layer of a three-dimensional mannequin of human dermis.
Furthermore, the applying of the prototype cream containing C. fragile EV (remaining 5 μg/ml) yielded 1.31% enchancment in pores and skin brightness in a scientific trial. Collectively, these outcomes recommend that EV from C. fragile and S. fusiforme scale back melanin synthesis and could also be potential therapeutic and/or supplementary whitening brokers.

Evaluating Whether or not Radiofrequency Irradiation Attenuated UV-B-Induced Pores and skin Pigmentation by Growing Melanosomal Autophagy and Reducing Melanin Synthesis

Autophagy is concerned within the degradation of melanosomes and the dedication of pores and skin shade. TLR4 and tumor necrosis issue (TNF) signaling upregulates NF-kB expression, which is concerned within the upregulation of mTOR. The activation of mTOR by UV-B publicity ends in decreased autophagy, whereas radiofrequency (RF) irradiation decreases TLR4 and TNF receptor (TNFR) expression.
We evaluated whether or not RF decreased pores and skin pigmentation by restoring autophagy by lowering the expression of TLR4 or TNFR/NF-κB/mTOR within the UV-B-irradiated animal mannequin. UV-B radiation induced the expressions of TNFR, TLR, and NF-κB within the pores and skin, which had been all decreased by RF irradiation. RF irradiation additionally decreased phosphorylated mTOR expression and upregulated autophagy initiation components equivalent to FIP200, ULK1, ULK2, ATG13, and ATG101 within the UV-B-irradiated pores and skin.
Beclin 1 expression and the expression ratio of LC3-I to LC3-II had been elevated by UV-B/RF irradiation. Moreover, melanin-containing autophagosomes elevated with RF irradiation. Fontana-Masson staining confirmed that the quantity of melanin deposition within the pores and skin was decreased by RF irradiation. This research confirmed that RF irradiation decreased pores and skin pigmentation by restoring melanosomal autophagy, and that the doable sign pathways which modulate autophagy may very well be TLR4, TNFR, NF-κB, and mTOR.

Cajanin Suppresses Melanin Synthesis via Modulating MITF in Human Melanin-Producing Cells

Regardless of its classification as a non-life-threatening illness, elevated pores and skin pigmentation adversely impacts high quality of life and results in lack of self-confidence. Till now, there aren’t any beneficial treatments with excessive efficacy and human security for hyperpigmentation.
This research aimed to analyze anti-melanogenic exercise and underlying mechanism of cajanin, an isoflavonoid extracted from Dalbergia parviflora Roxb. (Leguminosae) in human melanin-producing cells. Tradition with 50 μM cajanin for 48-72 h considerably suppressed proliferation in human melanoma MNT1 cells assessed by way of MTT viability assay. Curiously, cajanin additionally effectively diminished melanin content material in MNT1 cells with the half most inhibitory focus (IC50) at 77.47 ± 9.28 μM.

Melanin

GK4761-250 250
EUR 95

Melanin

GK4761-250MG 250 mg
EUR 151.2

Melanin

HY-113485 10mg
EUR 170.4

Melanin probe-1

T38855-10mg 10mg Ask for price
Description: Melanin probe-1

Melanin probe-1

T38855-1g 1g Ask for price
Description: Melanin probe-1

Melanin probe-1

T38855-1mg 1mg Ask for price
Description: Melanin probe-1

Melanin probe-1

T38855-50mg 50mg Ask for price
Description: Melanin probe-1

Melanin probe-1

T38855-5mg 5mg Ask for price
Description: Melanin probe-1

Melanin Bleach Kit

24883-1 1kit
EUR 187

Melanin Control Slides

HCS1014-25 25 Slides
EUR 137.81

Melanin (Control Slides)

TCS0015-100 100 Slides
EUR 847.2

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 278.4

Melanin (Control Slides)

TCS0015-5 5 Slides
EUR 117.6

Rat melanin ELISA Kit

ELK0492-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat melanin ELISA Kit

ELK0492-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat Melanin ELISA kit

E01A14866 96T
EUR 700
Description: ELISA

Goat Melanin ELISA kit

E01A49772 96T
EUR 700
Description: ELISA

Fish Melanin ELISA Kit

EK8732 96Т
EUR 799

Fish Melanin ELISA Kit

AE30071FI-48Tests 48 Tests
EUR 360
Description: Fish

Fish Melanin ELISA Kit

AE30071FI-96Tests 96 Tests
EUR 680
Description: Fish

Sheep Melanin ELISA kit

E01A102066 96T
EUR 700
Description: ELISA

Human Melanin ELISA kit

E01A6119 96T
EUR 700
Description: ELISA

Mouse Melanin ELISA kit

E01A23613 96T
EUR 700
Description: ELISA

Human melanin ELISA Kit

ELK8367-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

Human melanin ELISA Kit

ELK8367-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse melanin ELISA kit

ELK8565-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse melanin ELISA kit

ELK8565-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

Human melanin ELISA kit

EKC34570-48T 48T
EUR 535.99

Human melanin ELISA kit

EKC34570-5x96T 5x96T
EUR 3637.08

Human melanin ELISA kit

EKC34570-96T 96T
EUR 765.7

Human melanin ELISA kit

CSB-E14051h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativecompetitive ELISA kit for measuring Human melanin in samples from serum. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human melanin ELISA kit

1-CSB-E14051h
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Human melanin in samples from serum. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Melanin ELISA Kit

AE30027HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)
As an alternative of direct inactivating enzymatic perform of human tyrosinase, down-regulated mRNA and protein expression ranges of MITF and downstream melanogenic enzymes, together with tyrosinase, TRP-1 and Dct (TRP-2) had been noticed in MNT1 cells handled with 50 μM cajanin for 24-72 h. Correspondingly, therapy with cajanin modulated the signaling pathway of CREB and ERK which each regulate MITF expression stage. Focused suppression on MITF-related proteins in human melanin-producing cells strengthens the potential improvement of cajanin as an efficient therapy for human hyperpigmented problems.

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