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Protein profile of FAdV-4 based on SDS-PAGE and Western blot isolated from chickens in India.
Lieven
- 0
Fowl adenovirus-4 (FAdV-4) causes hydropericardium syndrome (HPS) in poultry worldwide. An understanding of viral structural protein composition is vital for growing novel immunodiagnostics and immunoprophylactics. Right here we report isolation, tradition, molecular and protein profile of FAdV-Four isolates recovered from HPS outbreaks in rooster within the states of Himachal Pradesh and Tamil Nadu in India.
We carried out a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting-based protein profiling of FAdV-Four isolates towards a reference FAdV-1 or Hen Embryo Deadly Orphan (CELO) virus. SDS-PAGE evaluation confirmed that seven protein bands in FAdV-Four isolates had been much like CELO anticipate a further band of 110 kDa in CELO virus.
On Western blotting, two protein fractions of 43 kDa and 78 kDa dimension had been noticed in FAdV-Four isolates. Total, outcomes present that FAdV-Four isolates recovered from completely different areas of the nation had comparable protein profile and presumably a typical supply of origin.
Number of Particular Peptides for Coccidioides spp. Obtained from Antigenic Fractions via SDS-PAGE and Western Blot Strategies by the Recognition of Sera from Sufferers with Coccidioidomycosis.
Antigenic fractions of 100, 50, 37, and 28 kDa obtained via the SDS-PAGE technique that had been extra regularly acknowledged by anti-Coccidioides antibodies within the sera of coccidioidomycosis sufferers had been chosen utilizing western blotting. Subsequently, these bands had been sequenced, and the obtained proteins had been analysed by BLAST to decide on peptides particular for Coccidioides spp.
from among the many shared aligned sequences of associated fungi. A peptide particular for C. immitis was chosen from the “GPI anchored serine-threonine wealthy protein OS C. immitis“, whereas from the “uncharacterized protein of C. immitis“, we chosen a peptide for C. immitis and C. posadasii. These proteins arose from the 100 kDa antigenic fraction.
From the protein “fatty acid amide hydrolase 1 of C. posadasii” that was recognized from the 50 kDa antigenic fraction, a peptide was chosen that acknowledged C. immitis and C. posadasii. As well as, the evaluation of all of the peptides (353) of every of the assembled proteins confirmed that solely 35 had 100% id with proteins of C. immitis and C. posadasii, one had 100% id with solely C. immitis, and one had 100% id with solely C. posadasii. These peptides can be utilized as diagnostic reagents, vaccines, and antifungals.

Willpower of the bovine meals allergen casein in white wines by quantitative oblique ELISA, SDS–PAGE, Western blot and immunostaining.
This research describes the characterization of allergic bovine casein and caseinate fining brokers by SDS-PAGE evaluation and the event of a quantitative oblique ELISA for the detection of those substances in wines. The ELISA was utilized to numerous experimental wines that had been handled with completely different caseinate dosages and went via completely different processing steps and to a panel of economic wines.
Optimistic outcomes had been assured by SDS-PAGE, Western blot, and immunostaining. Complete literature analysis was completed to guage the demanded sensitivity of the ELISA. The outcomes confirmed that alpha- and beta-caseins stay in some wines and are detectable.
Estimated quantities had been within the vary or under an estimated no-observed opposed impact degree (NOAEL) of 0.9 mg/L, but it surely was concluded that there’s nonetheless an uncertainty about this NOAEL. Further utilized processing, referring to bentonite remedy and successive filtration, was decided to contribute to a major lower of casein residues in wines.
A novel technique of loading samples onto mini-gels for SDS–PAGE: elevated sensitivity and Western blots utilizing sub-microgram portions of protein.
Commercially obtainable mini-gels for sodium dodecyl sulphate (SDS)-PAGE and Western blotting are restricted each by the variety of lanes that may be loaded per gel and the minimal quantity of protein per lane that should be loaded. Right here we describe a technique for loading protein samples onto current commercially obtainable mini-gels that enables loading of 50 or extra lanes per gel.
The improved sensitivity of the strategy permits Western blotting with sub-microgram portions of protein. Samples are loaded onto filter paper strips mounted on a plastic backing sheet, and film-wrapped strips on a separate dummy loader interdigitate with the pattern strips, making a bodily barrier to lateral diffusion. The pattern loader sandwich is positioned on high of the stacking gel, and is suitable with all commercially obtainable SDS-PAGE techniques.
Comparability of 15-lane mini-gels with 30-lane micro-loader strips reveals as much as a 10-fold improve in sensitivity with the brand new technique. Utilizing 50- and 66-lane micro-loaders, sub-microgram portions of protein produce dependable and quantifiable sign by Western blotting. Manipulation of the ionic circumstances inside dummy loader strips supplies a mechanism for enhancing lateral decision, permitting for the potential for additional miniaturization.
Canine pyometra: a research of the urinary proteins by SDS–PAGE and Western blot.
Canine pyometra typically causes glomerulonephritis by immune advanced deposition within the glomeruli. Proteinuria, starting from average to extreme, could also be current secondary to renal harm. To find out urinary protein excretion attributable to pyometra, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed on urine from 15 bitches with pyometra and 10 wholesome bitches.
To characterize urinary immunoglobin excretion, Western blot evaluation of the urine samples utilizing antibodies to canine IgG and IgA was additionally carried out. 9 bands had been detected by electrophoresis in bitches with pyometra, whereas solely 4 had been detected within the wholesome animals.
The urinary proteins from bitches with pyometra had been primarily of glomerular origin; 58% had been of medium-high molecular weight (MW), and the rest had been low MW. Not one of the wholesome canines had IgG or IgA of their urine, whereas three bitches with pyometra had IgG of their urine and one other bitch with pyometra had each IgG and IgA.
The low proportion of bitches with urinary immunoglobins was in all probability be attributable to early analysis of the illness. Though solely a restricted variety of canines was used, this research is outwardly the primary to characterize the electrophoretic sample of urinary proteins and to quantify urinary excretion of IgG and IgA in bitches with pyometra.
SDS–PAGE and Western blot of urinary proteins in canines with leishmaniasis.
Canine leishmaniasis is an endemic illness within the Mediterranean space attributable to the protozoan Leishmania infantum, which often produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot utilizing antibodies to IgG and IgA from canines had been carried out within the urine of 22 canines with leishmaniasis recognized by ELISA and confirmed by PCR, and 20 wholesome canines.
The outcomes had been in comparison with renal operate laboratory exams and to these from a histopathological research of the kidneys from sick animals that died naturally or had been euthanized. 5 completely different bands with molecular weights starting from 10 to 110 kDa had been obtained from the electrophoresis of the urine of wholesome canines. 33.5% of complete proteins corresponded to low molecular weight proteins and the opposite proteins had center and excessive molecular weights.
Nevertheless, within the group with leishmaniasis, a most of 11 completely different bands with molecular weights starting from 10 kDa to 150 kDa had been displayed within the electrophoresis of the urine. The urine electrophoretic sample within the sick canines was labeled as blended (proteins with excessive and low molecular weights) as a result of low molecular weight proteins made up 57.9% and the remainder of the proteins had center and excessive molecular weights.
SSB Western Blot kit (AWBK40462) |
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AWBK40462 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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OTC Western Blot kit (AWBK41766) |
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AWBK41766 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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FST Western Blot kit (AWBK42287) |
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AWBK42287 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TMB Substrate, for Western Blot |
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F100029 | Cygnus Technologies | 1000 ml | EUR 992.4 |
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
TMB Substrate, for Western Blot |
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F129-100 | Cygnus Technologies | 100 ml | EUR 298.8 |
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
ERG Western Blot kit (AWBK100625) |
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AWBK100625 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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GLI2 Western Blot kit (AWBK31885) |
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AWBK31885 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PAX4 Western Blot kit (AWBK32064) |
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AWBK32064 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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GLI1 Western Blot kit (AWBK32368) |
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AWBK32368 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PAX7 Western Blot kit (AWBK32393) |
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AWBK32393 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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LHX6 Western Blot kit (AWBK32553) |
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AWBK32553 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PAX7 Western Blot kit (AWBK32742) |
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AWBK32742 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SOX5 Western Blot kit (AWBK33323) |
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AWBK33323 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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A1BG Western Blot kit (AWBK33810) |
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AWBK33810 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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NPM1 Western Blot kit (AWBK34094) |
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AWBK34094 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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LIG4 Western Blot kit (AWBK34122) |
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AWBK34122 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RNF2 Western Blot kit (AWBK34290) |
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AWBK34290 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ILF2 Western Blot kit (AWBK35731) |
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AWBK35731 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SPP1 Western Blot kit (AWBK36677) |
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AWBK36677 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ZBP1 Western Blot kit (AWBK36711) |
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AWBK36711 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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G3BP Western Blot kit (AWBK37713) |
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AWBK37713 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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BRD7 Western Blot kit (AWBK39018) |
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AWBK39018 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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MSI2 Western Blot kit (AWBK41112) |
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AWBK41112 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TPM1 Western Blot kit (AWBK41392) |
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AWBK41392 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TSHR Western Blot kit (AWBK41870) |
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AWBK41870 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ASPN Western Blot kit (AWBK42487) |
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AWBK42487 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RORA Western Blot kit (AWBK45608) |
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AWBK45608 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PDCD4 Western Blot kit (AWBK09045) |
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AWBK09045 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ETS1 Western Blot kit (AWBK100604) |
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AWBK100604 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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GABRP Western Blot kit (AWBK13034) |
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AWBK13034 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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HTR1A Western Blot kit (AWBK13041) |
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AWBK13041 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RAB14 Western Blot kit (AWBK13107) |
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AWBK13107 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TNRC4 Western Blot kit (AWBK31525) |
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AWBK31525 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ESRRG Western Blot kit (AWBK31655) |
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AWBK31655 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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HIPK2 Western Blot kit (AWBK32586) |
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AWBK32586 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ZFP67 Western Blot kit (AWBK32749) |
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AWBK32749 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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OLIG2 Western Blot kit (AWBK32753) |
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AWBK32753 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SOX10 Western Blot kit (AWBK33326) |
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AWBK33326 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RPS16 Western Blot kit (AWBK33535) |
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AWBK33535 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ATP7A Western Blot kit (AWBK33797) |
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AWBK33797 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ZFP36 Western Blot kit (AWBK34385) |
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AWBK34385 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ARIH2 Western Blot kit (AWBK34418) |
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AWBK34418 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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KEAP1 Western Blot kit (AWBK34728) |
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AWBK34728 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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CLIC1 Western Blot kit (AWBK35045) |
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AWBK35045 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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KCNN2 Western Blot kit (AWBK35094) |
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AWBK35094 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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CLCN3 Western Blot kit (AWBK35504) |
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AWBK35504 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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P2RX7 Western Blot kit (AWBK35518) |
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AWBK35518 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TRPM3 Western Blot kit (AWBK35609) |
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AWBK35609 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PITX2 Western Blot kit (AWBK35634) |
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AWBK35634 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ZFP42 Western Blot kit (AWBK36244) |
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AWBK36244 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ANXA1 Western Blot kit (AWBK36568) |
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AWBK36568 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RUNX2 Western Blot kit (AWBK36678) |
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AWBK36678 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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Bapx1 Western Blot kit (AWBK36826) |
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AWBK36826 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SATB1 Western Blot kit (AWBK38208) |
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AWBK38208 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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JMJD3 Western Blot kit (AWBK40102) |
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AWBK40102 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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STAU1 Western Blot kit (AWBK40537) |
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AWBK40537 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PCBP1 Western Blot kit (AWBK40630) |
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AWBK40630 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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WNT2B Western Blot kit (AWBK41254) |
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AWBK41254 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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GNB1L Western Blot kit (AWBK41328) |
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AWBK41328 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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STK11 Western Blot kit (AWBK41743) |
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AWBK41743 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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KRT13 Western Blot kit (AWBK42031) |
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AWBK42031 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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IDH3A Western Blot kit (AWBK42237) |
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AWBK42237 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PBEF1 Western Blot kit (AWBK42255) |
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AWBK42255 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RSAD2 Western Blot kit (AWBK42696) |
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AWBK42696 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PANX2 Western Blot kit (AWBK42778) |
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AWBK42778 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PANX1 Western Blot kit (AWBK42783) |
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AWBK42783 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TGFBI Western Blot kit (AWBK44268) |
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AWBK44268 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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PTPN2 Western Blot kit (AWBK45362) |
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AWBK45362 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TWIST1 Western Blot kit (AWBK37997) |
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AWBK37997 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TARDBP Western Blot kit (AWBK38941) |
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AWBK38941 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TARDBP Western Blot kit (AWBK38942) |
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AWBK38942 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SNRP70 Western Blot kit (AWBK40439) |
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AWBK40439 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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HNRPLL Western Blot kit (AWBK41102) |
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AWBK41102 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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HNRPA3 Western Blot kit (AWBK41195) |
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AWBK41195 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SLC1A5 Western Blot kit (AWBK42247) |
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AWBK42247 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ONECUT2 Western Blot kit (AWBK35750) |
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AWBK35750 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SLC12A1 Western Blot kit (AWBK41388) |
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AWBK41388 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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CEACAM6 Western Blot kit (AWBK41504) |
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AWBK41504 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SLC22A1 Western Blot kit (AWBK41516) |
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AWBK41516 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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RHOBTB1 Western Blot kit (AWBK41883) |
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AWBK41883 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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SLC22A7 Western Blot kit (AWBK42306) |
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AWBK42306 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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Interferon alpha Western Blot Antibody |
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20-abx137014 | Abbexa |
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Colorimetric Western Blot Kit (OKRA00014) |
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OKRA00014 | Aviva Systems Biology | 1 kit | EUR 730.8 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00015) |
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OKRA00015 | Aviva Systems Biology | 1 each | EUR 730.8 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00016) |
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OKRA00016 | Aviva Systems Biology | 1 kit | EUR 730.8 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00017) |
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OKRA00017 | Aviva Systems Biology | 1 kit | EUR 730.8 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
cyclin G2 Western Blot kit (AWBK03032) |
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AWBK03032 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ChonBlock Western Blot Buffer 1 (PBS) |
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9069P | Chondrex | 10 ml | EUR 232.26 |
Description: ChonBlock Western Blot Buffer 1 (PBS) |
ChonBlock Western Blot Buffer 1 (TBS) |
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9069T | Chondrex | 10 ml | EUR 232.26 |
Description: ChonBlock Western Blot Buffer 1 (TBS) |
Pure-IP Western Blot Detection Kit |
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PRB-5002 | Cell Biolabs | 20 blots | EUR 470.4 |
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody. |
Chemiluminescent Western Blot Kit (OKRA00001) |
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OKRA00001 | Aviva Systems Biology | 1 kit | EUR 684 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Chemiluminescent Western Blot Kit (OKRA00002) |
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OKRA00002 | Aviva Systems Biology | 1 kit | EUR 684 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Chemiluminescent Western Blot Kit (OKRA00003) |
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OKRA00003 | Aviva Systems Biology | 1 kit | EUR 684 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Chemiluminescent Western Blot Kit (OKRA00004) |
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OKRA00004 | Aviva Systems Biology | 1 kit | EUR 684 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
T-Pro Western Blot Stripping Reagent |
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JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 213.6 |
AgileBlotter? Automated Western Blot Processor |
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ACT-ABH-40 | ACTGene | each | EUR 20326.8 |
Nitrated Mol Wt Std for Western blot |
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NITT12-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Western Blot Primary Antibody Diluent Buffer |
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abx090689-100ml | Abbexa | 100 ml | EUR 218.4 |
Attoglow Western Blot Analysis Kit:Blocking Agent |
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K3171250-3 | Biochain | 40 g | EUR 68 |
Hypersensitive Western Blot Chemiluminescent Substrate |
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CR0001-10 | BosterBio | 20mL (sufficient reagents for 4000 cm2 of membrane) | EUR 200.4 |
Hypersensitive Western Blot Chemiluminescent Substrate |
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CR0001-25 | BosterBio | 50mL (sufficient reagents for 10000 cm2 of membrane) | EUR 376.8 |
Hypersensitive Western Blot Chemiluminescent Substrate |
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CR0001-5 | BosterBio | 10mL (sufficient reagents for 2000 cm2 of membrane) | EUR 139.2 |
Recombinant Human MutY Protein for Western blot |
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MUTY11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
ChonBlock Western Blot Buffer 2 for Goat (PBS) |
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90691P | Chondrex | 10 ml | EUR 145.26 |
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS) |
ChonBlock Western Blot Buffer 2 for Goat (TBS) |
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90691T | Chondrex | 10 ml | EUR 145.26 |
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS) |
West-Ez Blocking Buffer, 1 % Casein for Western Blot |
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W3720-050 | GenDepot | 500ml | EUR 144 |
West-Ez Blocking Buffer, 1 % Casein for Western Blot |
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W3720-100 | GenDepot | 2x500ml | EUR 176.4 |
Poly-ADP-ribosylated proteins for Western blot |
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ADPR11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Rat Ceruloplasmin protein control for western blot |
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CP17-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Attoglow Western Blot Analysis Kit:Binding Buffer 20x |
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K3171250-2 | Biochain | 120 ml | EUR 107 |
SuperBrite? ECL Western Blot Substrate/Detection Kit |
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K824-50 | Biovision | each | EUR 229.2 |
ChonBlock Western Blot Buffer 2 for Rabbit (PBS) |
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90692P | Chondrex | 10 ml | EUR 145.26 |
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS) |
ChonBlock Western Blot Buffer 2 for Rabbit (TBS) |
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90692T | Chondrex | 10 ml | EUR 145.26 |
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS) |
Bovine DNAse 1 Protein Western blot +ve control |
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DNASE11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Mouse Leptin protein Western blot +ve control # 1 |
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LEP11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Leptin protein Western blot +ve control # 2 |
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LEP12-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Mouse Ceruloplasmin protein control for western blot |
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CP16-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Western blot recycling stripping buffer, 50 ml (10X) |
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90101 | Alpha Diagnostics | 50 ml | EUR 285.6 |
Box Western Blot Polystyrene Transparent 95x30x16mm X5 |
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DD62676 | Scientific Laboratory Supplies | PK5 | EUR 99.6 |
Attoglow Western Blot Analysis Kit: Millennium Enhancer |
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K3171250-1 | Biochain | 100 ml | EUR 130 |
Purified Human C1q protein Western blot +ve control |
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C1Q11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
HRP Western Blot Anti-IgG Antibody Kit (OKRA00006) |
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OKRA00006 | Aviva Systems Biology | 100 ug | EUR 246 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Human C1 Esterase Inhibitor western blot +ve control |
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C1E11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection |
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ACT-WP | ACTGene | each | EUR 172.8 |
Human Liver Ferritin protein Western blot +ve control |
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FERT12-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls |
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80215-250 | Alpha Diagnostics | 1 kit | EUR 196.8 |
Beta-Galactosidase (E. Coli) Protein for Western Blot |
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BGAL11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Spleen Ferritin protein Western blot +ve control |
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FERT11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Horse Spleen Ferritin protein Western blot +ve control |
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FERT13-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Chicken Serum Albumin protein control for Western blot |
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ALBC15-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Purified Cholera Toxin protein control for Western Blot |
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CTOX11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Pancreatic Amylase protein control for Western blot |
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PAMY13-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Western Blot Box - 6 x 4 x 2in.; 15.2 x 10.2 x 5.1cm |
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B1200-19 | MTC Bio | 5/pack | EUR 108.6 |
Description: Western Blot Boxes |
Middle range pre-stained (blue) protein markers (19-110 kda) for Western (SDS-PAGE) |
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MMWM-14 | Alpha Diagnostics | 200 ul | EUR 196.8 |
Camelpox virus H3L/p35 protein control for western blot |
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CPOX11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Adipsin/Factor D protein Western blot +ve control |
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ADN12-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Zika Virus prM Protein (African) control for Western blot |
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ZPRM11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Recombinant Beta Lactamase protein control for Western blot |
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BLAC11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Foot and mouth disease virus serotype O, 3D (FMDO-3D) protein control for western blot |
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FMDO3D11-C | Alpha Diagnostics | 100 ul | EUR 416.4 |
Human Liver Cathepsin B protein control for Western blot |
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CTHB11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Liver Cathepsin D protein control for Western blot |
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CTHD11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Liver Cathepsin G protein control for Western blot |
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CTHG11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Liver Cathepsin H protein control for Western blot |
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CTHH11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human Liver Cathepsin L protein control for Western blot |
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CTHL11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
HRP Western Blot Anti-Rabbit IgG Antibody Kit (OKRA00007) |
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OKRA00007 | Aviva Systems Biology | 100 ug | EUR 246 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
NuGlo (luminol) ECL Substrate Kit for Western blot, 50 mls |
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80215-250-S | Alpha Diagnostics | 1 kit | EUR 169.2 |
DNP-labeled Molecular weight standard proteins (21, 29, 43, 68 , and 97 kda) for Western blot |
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DNP45-C | Alpha Diagnostics | 1 set | EUR 343.2 |
Recombinant human BAFF-R protein control for Western blot |
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BAFFR11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Purified rat serum albumin protein control for Western blot |
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ALBR12-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control |
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COX11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control |
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COX21-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Biotin Western Blot Anti-IgG Antibody MaxTag Kit (OKRA00023) |
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OKRA00023 | Aviva Systems Biology | 1 ml | EUR 339.6 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Purified Mouse serum albumin protein control for Western blot |
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ALB11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Purified Human serum albumin protein control for Western blot |
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ALBH13-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Purified Sheep serum albumin protein control for Western blot |
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ALBSH20-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Western Blot Box - 6 x 4 x 1 1/4in.; 15.2 x 10.2 x 3.2cm |
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B1200-17 | MTC Bio | 5/pack | EUR 107.63 |
Description: Western Blot Boxes |
Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate |
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80203-Rt | Alpha Diagnostics | 1 kit | EUR 781.2 |
Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate |
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80209-Pg | Alpha Diagnostics | 1 kit | EUR 781.2 |
Mouse NOS-II/i-NOS Protein Western Blot +ve Control # 1 |
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iNOS-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate |
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80201-Gt | Alpha Diagnostics | 1 kit | EUR 781.2 |
Human recombinant, purified BMP2 protein Western blot +ve control |
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BMP21-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human recombinant, purified BMP3 protein Western blot +ve control |
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BMP31-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human recombinant, purified BMP4 protein Western blot +ve control |
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BMP41-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human recombinant, purified BMP5 protein Western blot +ve control |
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BMP51-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Human recombinant, purified BMP6 protein Western blot +ve control |
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BMP61-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate |
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80202-Mo | Alpha Diagnostics | 1 kit | EUR 781.2 |
×
In Western blot, not one of the wholesome canines confirmed excretion of IgG and/or IgA, whereas IgG and IgA had been detected within the Western blot of urine of 68% and 55% respectively of canines with leishmaniasis. The outcomes obtained within the leishmaniasis group agreed with glomerular and tubular harm, which had been confirmed by the histopathological findings.
Tags: 3xflag peptide cdna plasmid cgmp elisa cloning plasmid cmyc tag sequence co transfection protocol crispr cas plasmid crispr transfection protocol dna sequence analysis software gipz vector map his tag purification kit lentivirus packaging lentivirus packaging plasmids lentivirus titer microrna expression vector mlui restriction enzyme packaging plasmid pcdna3 1 hygro pcdna3 1 sequencing primers pcdna3 1 vector pegfp n1 pegfpn1 sequence pet sequencing primers pet vector maps pgl3 luciferase plasmid library puc57 genscript renilla luciferase plasmid rna to cdna strep ii tag sequence strepii tag sequence sure competent cell tac polymerase taq polymerase vsvg plasmid