tube ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) as Tools to Explore Ubiquitin-Proteasome System and PROTAC Drug Discovery

Tandem Ubiquitin Binding Entities (TUBEs) as Tools to Explore Ubiquitin-Proteasome System and PROTAC Drug Discovery

The ubiquitin proteasome system (UPS) is a fancy pathway that entails a number of enzymes and culminates within the formation of a polyubiquitin chain on a goal protein. As its significance is turning into extra evident in drug discovery, there’s a renewed curiosity in understanding the function that polyubiquitin chains play.
This has been a problem, largely because of the lack of experimental instruments for detecting the polyubiquitinated types of a protein of curiosity (POI). Tandem Ubiquitin Binding Entities (TUBEs) are engineered protein domains that bind particularly to polyubiquitin chains. These polyubiquitin affinity matrices are extremely delicate as they bind to polyubiquitin chains within the nanomolar vary.
They exist in two kinds: pan-selective TUBEs and chain-selective TUBEs. The flexibility of TUBEs to be conjugated to totally different entities is really what makes them distinctive. TUBEs are utilized in all kinds of experiments comparable to in protein pulldowns to complement for polyubiquitinated proteins. They’re an alternative choice to ubiquitin antibodies in Western blots.
Additional, TUBEs are used as seize reagents for immobilizing polyubiquitinated proteins on a microtiter plate. Using TUBEs as parts of in vitro and cell-based assays presents the distinctive function of confirming and assessing the polyubiquitination of a POI in response to inhibitors, activators, or PROTAC® molecules. Subsequently, TUBEs not solely play a giant function in finding out the usbut even have an enormous potential for rushing up the drug discovery course of.

A Novel Luminescence-Primarily based Excessive-Throughput Method for Mobile Decision of Protein Ubiquitination Utilizing Tandem Ubiquitin Binding Entities (TUBEs).

Protein turnover is extremely regulated by the posttranslational strategy of ubiquitination. Deregulation of the ubiquitin proteasome system (UPS) has been implicated in most cancers and neurodegenerative illnesses, and modulating this method has confirmed to be a viable strategy for therapeutic intervention. The event of novel applied sciences that allow high-throughput research of substrate protein ubiquitination is essential for UPS drug discovery.
Typical approaches for finding out ubiquitination both have excessive protein necessities or depend on exogenous or modified ubiquitin moieties, thus limiting their utility. As a way to circumvent these points, we developed a high-throughput live-cell assay that mixes the NanoBiT luminescence-based expertise with tandem ubiquitin binding entities (TUBEs) to resolve substrate ubiquitination.
To reveal the effectiveness and utility of this assay, we studied compound-induced ubiquitination of the G to S Part Transition 1 (GSPT1) protein. Utilizing this assay, we characterised compounds with various ranges of GSPT1 ubiquitination exercise.
This methodology gives a live-cell-based strategy for assaying substrate ubiquitination that may be tailored to review the kinetics of ubiquitin switch onto a substrate protein of curiosity. As well as, our outcomes present that this strategy is transportable for finding out the ubiquitination of goal proteins with various features.

New insights into host-parasite ubiquitin proteome dynamics in P. falciparum contaminated purple blood cells utilizing a TUBEs-MS strategy.

Malaria, attributable to Plasmodium falciparum (P. falciparum), ranks as some of the baleful infectious illnesses worldwide. New antimalarial therapies are wanted to face present or rising drug resistant strains. Protein degradation seems to play a big function through the asexual intraerythrocytic developmental cycle (IDC) of P. falciparum.
Inhibition of the ubiquitin proteasome system (UPS), a serious intracellular proteolytic pathway, successfully reduces an infection and parasite replication. P. falciparum and erythrocyte UPS coexist throughout IDC however the nature of their relationship is essentially unknown. We used an strategy primarily based on Tandem Ubiquitin-Binding Entities (TUBEs) and 1D gel electrophoresis adopted by mass spectrometry to determine main parts of the TUBEs-associated ubiquitin proteome of each host and parasite throughout ring, trophozoite and schizont levels.
Ring-exported protein (REX1), a P. falciparum protein situated in Maurer’s clefts and vital for parasite nutrient import, was discovered to succeed in a most stage of ubiquitylation in trophozoites stage. The Homo sapiens (H. sapiens) TUBEs related ubiquitin proteome decreased through the an infection, whereas the equal P. falciparum TUBEs-associated ubiquitin proteome counterpart elevated. Main mobile processes comparable to DNA restore, replication, stress response, vesicular transport and catabolic occasions look like regulated by ubiquitylation alongside the IDC P. falciparum an infection.

The Hectd1 ubiquitin ligase is required for growth of the top mesenchyme and neural tube closure.

Closure of the cranial neural tube is determined by regular growth of the top mesenchyme. Homozygous-mutant embryos for the ENU-induced open thoughts (opm) mutation exhibit exencephaly related to defects in head mesenchyme growth and dorsal-lateral hinge level formation. The top mesenchyme in opm mutant embryos is denser than in wildtype embryos and shows an irregular mobile group.
Since cells that originate from each the cephalic paraxial mesoderm and the neural crest populate the top mesenchyme, we explored the origin of the irregular head mesenchyme. opm mutant embryos present apparently regular growth of neural crest-derived constructions. Moreover, the irregular head mesenchyme in opm mutant embryos will not be derived from the neural crest, however as an alternative expresses molecular markers of cephalic mesoderm.
We additionally report the identification of the opm mutation within the ubiquitously expressed Hectd1 E3 ubiquitin ligase. Two totally different Hectd1 alleles trigger incompletely penetrant neural tube defects in heterozygous animals, indicating that Hectd1 perform is required at a important threshold for neural tube closure. This low penetrance of neural tube defects in embryos heterozygous for Hectd1 mutations means that Hectd1 needs to be thought-about as candidate susceptibility gene in human neural tube defects.
tube ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) as Tools to Explore Ubiquitin-Proteasome System and PROTAC Drug Discovery

A quick stream tube examine of fuel section H/D trade of multiply protonated ubiquitin.

An electrospray ionization (ESI)/fast-flow method has been utilized to the examine of fuel section hydrogen/deuterium (H/D) trade kinetics. Multiply charged ubiquitin ions [ubiquitin + nH](n)(+), in cost states n = 7-13, have been reacted with ND(3). The conduct of ND(3) as trade reagent is totally different from that of the beforehand studied reagents, D(2)O and CH(3)OD.
Opposite to these, the utmost variety of exchanged hydrogen atoms and the general trade fee have been noticed to extend with rising cost state of the ubiquitin ions. The outcomes are reagent-dependent as a result of the trade mechanisms are totally different for the totally different reagents. This remark is in settlement with a current conclusion by Beauchamp and colleagues that opposite to the idea usually expressed in earlier research, H/D trade kinetics could indirectly replicate ion constructions.
The outcomes for all three reagents are, nonetheless, according to observations of earlier ion mobility experiments that with rising cost state the conformers change from extra compact, partially folded constructions to elongated practically linear ones. H/D trade of (ubiquitin + 13H)(13+) with ND(3) results in two separated ion populations reflecting the potential existence of two conformers with totally different trade charges. The ions (ubiquitin + 8H)(8+) and (ubiquitin + 11H)(11+) signify {a partially} folded construction and an unfolded construction, respectively, and have been studied in better element.
The relative abundances of ions have been measured in steps of 0.5 m/z (mass-to-charge ratio), as a perform of the ND(3) stream fee. The experimental outcomes have been simulated by laptop fitted curves primarily based on a not too long ago developed algorithm. The algorithm permits the extraction of units of grouped fee constants. Eight fee fixed teams have been deduced for every of the 2 ions.

ubiquitin (Ubiquitin) Antibody (ALP)

abx442291-200ug 200 ug
EUR 744

ubiquitin (Ubiquitin) Antibody (ALP)

abx442300-200ug 200 ug
EUR 744

ubiquitin (Ubiquitin) Antibody (APC)

abx442572-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (APC)

abx442581-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (HRP)

abx443413-200ug 200 ug
EUR 727.2

ubiquitin (Ubiquitin) Antibody (HRP)

abx443422-200ug 200 ug
EUR 727.2

ubiquitin (Ubiquitin) Antibody (ALP)

abx447954-200ul 200 ul
EUR 744

ubiquitin (Ubiquitin) Antibody (APC)

abx447955-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (HRP)

abx447958-200ul 200 ul
EUR 727.2

ubiquitin (Ubiquitin) Antibody (RPE)

abx447961-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ALP)

abx444810-100ug 100 ug
EUR 477.6

ubiquitin (Ubiquitin) Antibody (APC)

abx444814-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (HRP)

abx444826-100ug 100 ug
EUR 477.6

ubiquitin (Ubiquitin) Antibody (RPE)

abx444838-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (RPE)

abx444256-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (RPE)

abx444265-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (FITC)

abx443133-200ug 200 ug
EUR 744

ubiquitin (Ubiquitin) Antibody (FITC)

abx443142-200ug 200 ug
EUR 744

ubiquitin (Ubiquitin) Antibody (FITC)

abx447957-200ul 200 ul
EUR 744

ubiquitin (Ubiquitin) Antibody (FITC)

abx444822-100ug 100 ug
EUR 477.6

ubiquitin (Ubiquitin) Antibody (PerCP)

abx447960-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (PerCP)

abx444834-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (PerCP)

abx443975-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (PerCP)

abx443984-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (Biotin)

abx442853-200ug 200 ug
EUR 744

ubiquitin (Ubiquitin) Antibody (Biotin)

abx442862-200ug 200 ug
EUR 744

ubiquitin (Ubiquitin) Antibody (Biotin)

abx447956-200ul 200 ul
EUR 744

ubiquitin (Ubiquitin) Antibody (Biotin)

abx444818-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 390)

abx440043-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 390)

abx440052-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 488)

abx440324-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 488)

abx440333-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 565)

abx440605-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 565)

abx440614-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 594)

abx440886-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 594)

abx440895-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 633)

abx441167-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 633)

abx441176-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 655)

abx441448-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 655)

abx441457-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 680)

abx441729-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 680)

abx441738-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 700)

abx442010-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 700)

abx442019-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 390)

abx447946-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 488)

abx447947-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 565)

abx447948-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 594)

abx447949-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 633)

abx447950-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 655)

abx447951-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 680)

abx447952-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 700)

abx447953-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (ATTO 655)

abx444798-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 680)

abx444802-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 700)

abx444806-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 390)

abx444778-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 488)

abx444782-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 565)

abx444786-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 594)

abx444790-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (ATTO 633)

abx444794-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (Streptavidin)

abx447962-200ul 200 ul
EUR 760.8

ubiquitin (Ubiquitin) Antibody (Streptavidin)

abx444842-100ug 100 ug
EUR 493.2

ubiquitin (Ubiquitin) Antibody (Streptavidin)

abx444537-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (Streptavidin)

abx444546-200ug 200 ug
EUR 760.8

ubiquitin (Ubiquitin) Antibody (PE/ATTO 594)

abx443694-200ug 200 ug
EUR 777.6

ubiquitin (Ubiquitin) Antibody (PE/ATTO 594)

abx443703-200ug 200 ug
EUR 777.6

ubiquitin (Ubiquitin) Antibody (PE/ATTO 594)

abx447959-200ul 200 ul
EUR 777.6

ubiquitin (Ubiquitin) Antibody (PE/ATTO 594)

abx444830-100ug 100 ug
EUR 510

Ubiquitin

E8ER31212 100ul
EUR 275
Description: Available in various conjugation types.

Ubiquitin

E8ET1609-21 100ul
EUR 275
Description: Available in various conjugation types.

UBIQUITIN

GWB-693DA3 0.25 mg Ask for price

Ubiquitin

GWB-DF6295 0.01 mg Ask for price

Ubiquitin

PR27083 50 ug
EUR 381.6

Ubiquitin

pro-314 10µg
EUR 60
Description: Recombinant Human Ubiquitin

Ubiquitin

MO18001 100 ul
EUR 579.6

Ubiquitin

MO22152 100 ul
EUR 522

Ubiquitin

PR27083-B each
EUR 184.5

Ubiquitin

RA22154 each
EUR 296.1

Ubiquitin D

E8ET1701-9 100ul
EUR 275
Description: Available in various conjugation types.

Ubiquitin D

AP89065 1mg
EUR 2640

Ubiquitin D

AP89066 1mg
EUR 2640

Ubiquitin+1

MO15045 500 ug
EUR 615.6

Ubiquitin+1

MO15138 500 ug
EUR 732

Ubiquitin+1

RA15043 100 ug
EUR 631.2

Ubiquitin AMC

81150 50 µg
EUR 380
Description: Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.

Ubiquitin G76A

pro-280 5µg
EUR 60
Description: Recombinant Human Ubiquitin Gly76 to Ala76 mutation

Ubiquitin K48R

pro-372 5µg
EUR 60
Description: Recombinant Human Ubiquitin Lys 48 to Arg 48 mutation

Ubiquitin-AMC

4842-25 each
EUR 366

Ubiquitin Biotin

pro-629 2µg
EUR 60
Description: Recombinant Human Ubiquitin Biotin

Ubiquitin protein

30R-1044 100 ug
EUR 219
Description: Purified recombinant Human Ubiquitin protein

Ubiquitin protein

30R-2726 100 ug
EUR 311
Description: Purified recombinant Human Ubiquitin protein

Ubiquitin protein

30R-2727 25 ug
EUR 335
Description: Synthetic Ubiquitin protein

Ubiquitin protein

30R-2730 50 ug
EUR 511
Description: Purified recombinant Human Ubiquitin protein

Ubiquitin protein

30R-3009 500 ug
EUR 268.8
Description: Purified recombinant Ubiquitin protein

Ubiquitin Protein

20-abx263565
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 2 µg
  • 5 ug

Ubiquitin Protein

20-abx260431
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 1 mg
  • 50 ug

Ubiquitin Antibody

20-abx100215
  • Ask for price
  • Ask for price
  • 1 mg
  • 200 ug

Ubiquitin Antibody

8C0385 50ug
EUR 368
Description: Ubiquitin Antibody

Ubiquitin antibody

10R-U101b 100 ug
EUR 561
Description: Mouse monoclonal Ubiquitin antibody

Ubiquitin antibody

10R-6608 100 ug
EUR 205
Description: Mouse monoclonal Ubiquitin antibody

Ubiquitin antibody

10R-7469 100 ul
EUR 471.6
Description: Mouse monoclonal Ubiquitin antibody

Ubiquitin antibody

10R-7470 100 ul
EUR 471.6
Description: Mouse monoclonal Ubiquitin antibody

Ubiquitin antibody

20-UR20 200 ul
EUR 135
Description: Rabbit polyclonal Ubiquitin antibody

Ubiquitin antibody

20R-2923 100 ul
EUR 471.6
Description: Rabbit polyclonal Ubiquitin antibody

Ubiquitin Antibody

33537 100ul
EUR 319

Ubiquitin Antibody

33537-100ul 100ul
EUR 302.4

Ubiquitin Antibody

33537-50ul 50ul
EUR 224.4

Ubiquitin Antibody

48860 100ul
EUR 499

Ubiquitin Antibody

48860-100ul 100ul
EUR 399.6

Ubiquitin Antibody

48860-50ul 50ul
EUR 286.8

ubiquitin Antibody

39382-100ul 100ul
EUR 468

Ubiquitin Antibody

20-abx159758
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

Ubiquitin Antibody

20-abx159759
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  • Ask for price
  • 100 ug
  • 50 ug

Ubiquitin Antibody

abx448822-100ug 100 ug
EUR 727.2

Ubiquitin Antibody

abx448840-100ug 100 ug
EUR 727.2

Ubiquitin Antibody

abx448876-100ug 100 ug
EUR 727.2

Ubiquitin Antibody

E033537 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Ubiquitin Antibody

E11-0385C 100μg
EUR 225
Description: Available in various conjugation types.

Ubiquitin Antibody

E18-0289-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Ubiquitin Antibody

E18-0289-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Ubiquitin Antibody

E90125 100ul
EUR 225
Description: Available in various conjugation types.

Ubiquitin Antibody

E38PA5909 100ul
EUR 225
Description: Available in various conjugation types.

ubiquitin Antibody

E300948 100ug/200ul
EUR 275
Description: Available in various conjugation types.

Ubiquitin antibody

70R-13961 100 ug
EUR 482
Description: Affinity purified Rabbit polyclonal Ubiquitin antibody

Ubiquitin antibody

70R-10670 1 ml
EUR 368
Description: Rabbit polyclonal Ubiquitin antibody

Ubiquitin antibody

70R-30827 100 ug
EUR 294
Description: Rabbit polyclonal Ubiquitin antibody

Ubiquitin antibody

70R-UR007 100 ug
EUR 280
Description: Affinity purified Rabbit polyclonal Ubiquitin antibody

Ubiquitin Antibody

AF0289-100ul 100ul
EUR 280

Ubiquitin Antibody

AF0289-200ul 200ul
EUR 350

Ubiquitin Antibody

ABF0289 100 ug
EUR 525.6

Ubiquitin Antibody

GWB-641C1B 0.1 mg Ask for price

Ubiquitin, Antibody

GWB-210C30 0.1 mg Ask for price

Ubiquitin, Antibody

GWB-2ED250 1 ml Ask for price

Ubiquitin, Antibody

GWB-1AF86D 0.1 mg Ask for price

Ubiquitin Antibody

GWB-82C9CF 0.1 ml Ask for price

Ubiquitin, Antibody

GWB-879374 7 ml Ask for price

Ubiquitin Antibody

GWB-98EAEE 0.1 ml Ask for price

UBIQUITIN, Antibody

GWB-7298EC 0.1 mg Ask for price

Ubiquitin Antibody

GWB-E69CE0 0.5 ml Ask for price

Ubiquitin Antibody

GWB-F10E58 0.2 ml Ask for price

Ubiquitin, Antibody

GWB-D4345F 0.1 mg Ask for price

Ubiquitin Antibody

GWB-C0A4D0 0.02 mg Ask for price

Ubiquitin Antibody

F42055-0.08ML 0.08 ml
EUR 140.25
Description: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. It is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.

Ubiquitin Antibody

F42055-0.4ML 0.4 ml
EUR 330.65
Description: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. It is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.

Ubiquitin Antibody

F42064-0.08ML 0.08 ml
EUR 140.25
Description: This gene encodes Ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Ubiquitin Antibody

F42064-0.4ML 0.4 ml
EUR 322.15
Description: This gene encodes Ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Ubiquitin Antibody

F52850-0.08ML 0.08 ml
EUR 140.25
Description: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Ubiquitin Antibody

F52850-0.4ML 0.4 ml
EUR 322.15
Description: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Ubiquitin Antibody

R30490 100 ug
EUR 356.15
Description: Ubiquitin(originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues(ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Ubiquitin Antibody

R31544 100 ug
EUR 356.15
Description: Ubiquitin (originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Ubiquitin Antibody

RQ5311 100ul
EUR 356.15
Description: The UBB gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. [RefSeq]

Ubiquitin Antibody

RQ5312 100ul
EUR 356.15
Description: The UBB gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. [RefSeq]

Ubiquitin Antibody

V8150-100UG 100 ug
EUR 349.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V8150-20UG 20 ug
EUR 153.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V8150SAF-100UG 100 ug
EUR 349.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V3894-100UG 100 ug
EUR 424.15
Description: Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or less frequently ubiquitylation. Ubiquitination affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade is to bind ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond. [Wiki]

Ubiquitin Antibody

V3894-20UG 20 ug
EUR 186.15
Description: Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or less frequently ubiquitylation. Ubiquitination affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade is to bind ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond. [Wiki]

Ubiquitin Antibody

V3894SAF-100UG 100 ug
EUR 424.15
Description: Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or less frequently ubiquitylation. Ubiquitination affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade is to bind ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond. [Wiki]

Ubiquitin Antibody

V3933-100UG 100 ug
EUR 349.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V3933-20UG 20 ug
EUR 153.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V3933SAF-100UG 100 ug
EUR 349.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V3934-100UG 100 ug
EUR 349.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V3934-20UG 20 ug
EUR 153.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

V3934SAF-100UG 100 ug
EUR 349.3
Description: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Ubiquitin Antibody

abx100215-100l 100 µl
EUR 275

Ubiquitin Antibody

abx100215-1ml 1 ml
EUR 750
These fee constants correspond to 32 and 44 H/D exchanges for the 8+ and 11+ charged ions, respectively. The outcomes point out increased particular person charges for many of the exchanged atoms within the 11+ ion in comparison with the 8+ ion.

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