Medical Mitogen-activated protein kinases (MAPK) Research

anti- Phospoho CDK, MAPK, GSK3, CLK, ERK1/2, JNK

all blue protein ladder, Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.
Ae1 Ae3 Antibody Bafilomycin A1 Bile Acid Assay Bioassay Blog cDNA Ch 223191 Choline Acetyltransferase Antibody Deoxycholic Acid Sodium Salt ELISA kit Epz 6438 exosome F Actin Antibody Ldn 193189 Medium Mip 1B Mmp Activity Assay Pamabrom 100Mg Pepstatin A peptide Phospho 4Ebp1 Polyclonal Sr 9011 Trypsin Activity Assay Tubastatin A Uric Acid Assay Valproic Acid Sodium Salt Western Blot

Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.

The biopolymer chitosan is at present in widespread use due to its nontoxicity, biocompatibility and biodegradability. Subsequently, on this examine, chitosan extracted from shrimp shells was used to synthesise biogenic silver nanoparticles (AgNPs). UV-visible spectrophotometry of lowered silver nanoparticles within the colloidal answer confirmed a single peak at 400 nm, confirming the formation of AgNPs.
The presence of biomolecules liable for lowering and capping the biogenic AgNPs was confirmed by FTIR. Floor morphology of the biosynthesised AgNPs was characterised utilizing SEM, and TEM evaluation confirmed the formation of spherical shapes 17-50 nm. The presence of elemental silver within the synthesised biogenic AgNPs was confirmed utilizing EDX and the crystalline construction characterised by XRD.
Cytotoxicity of biogenic AgNPs was decided utilizing MTT and the trypan blue exclusion assay. Morphological adjustments in HepG2 cells had been detected by evaluation of the DNA ladder sample by way of gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase three and 9 protein expression outcomes confirmed cell demise by way of apoptosis. In conclusion, chitosan has the flexibility to synthesise AgNPs with in vitro apoptotic actions.

Myricetin enhances on apoptosis induced by serum deprivation in PC12 cells mediated by mitochondrial signaling pathway.

Polyphenols have many useful results and an efficient illness therapeutic auxiliary drug. Beforehand, myricetin, a polyphenol, had been reported to own numerous organic results on human physiology. Nevertheless, mechanism of myricetin on apoptosis induced in PC12 cells remains to be unclear. PC12 cells had been handled with myricetin in two focus ranges comprising 0.1 and 1 μM beneath serum-free situation. Because of this, morphological adjustments had been noticed utilizing trypan blue assay.
DNA fragmentation was decided by DNA ladder assay to judge DNA harm ranges. Western blotting outcomes confirmed that cytosolic cytochrome c which was launched from mitochondria. Subsequently, tumor suppressor gene p53, pro-apoptotic and anti-apoptotic Bcl-2 household proteins Bax and Bcl-2 had been expressed. The caspase cascade response was induced via caspase three and 9 expression. From these outcomes, it’s advised that myricetin considerably enhanced the apoptosis induced by serum deprivation in a dose-dependent method in PC12 cells.

Mechanisms of chromium hexavalent-induced apoptosis in rat testes.

Hexavalent chromium (CrVI)-containing compounds, current in industrial settings and within the setting, are often known as carcinogens and mutagens. The current examine is designed to check the speculation that oxidative stress mediates CrVI-induced apoptosis in testis. Male Wistar rats obtained an intraperitoneal injection of potassium dichromate at doses of 1 and a pair of mg kg-1.
Superoxide anion manufacturing was assessed by the willpower of the discount of cytochrome c and iodonitrotetrazolium, lipid peroxidation (LPO), metallothioneins (MTs), and catalase (CAT) exercise. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis was detected by toluidine blue staining.
The expression of Bax and Bcl-2 proteins (Pts) was additionally investigated. After 15 days of therapy, a rise of LPO and MT ranges occurred, whereas CAT exercise was decreased. Testicular tissues of handled rats confirmed pronounced degradation of the DNA into oligonucleotides as seen within the typical electrophoretic DNA ladder sample. Intense apoptosis was noticed in germinal cells of Cr-exposed rats.
Bax Pt expression was induced in spermatogonia and spermatocytes cells of CrVI-treated rats. In distinction, Bcl-2 Pt was sometimes noticed in germ cells of CrVI-exposed rats. These outcomes clearly recommend that CrVI subacute therapy causes oxidative stress in rat testis resulting in apoptosis.

Upregulation of the MCL-1S protein variant following dihydroartemisinin therapy induces apoptosis in cholangiocarcinoma cells.

The intention of the current examine was to find out whether or not dihydroartemisinin (DHA) induces apoptosis within the human cholangiocarcinoma QBC939 cell line via the regulation of myeloid cell leukemia-1 (MCL-1) expression. The inhibitory charges of DHA on QBC939 cell proliferation and the consequences of DHA on the cell demise charges at numerous DHA concentrations and following numerous therapy instances had been examined.
The speed of apoptosis and cell cycle adjustments following DHA therapy had been examined and the adjustments within the expression of MCL-1 mRNAs and MCL-1 proteins following DHA therapy had been additionally examined. The MTT assay and trypan blue staining demonstrated that DHA considerably inhibited the proliferation (P<0.05) and promoted the demise of QBC939 cells (P<0.05).
The DNA ladder assay and circulate cytometry (FCM) evaluation demonstrated that the speed of apoptosis within the experimental group was considerably elevated following DHA therapy (P<0.01). FCM evaluation additionally demonstrated that DHA therapy led to a discount within the share of QBC939 cells within the G0/G1 and G2/M phases, and the vast majority of the DNA-treated cells had been arrested within the S part of the cell cycle (P<0.01).
Western blot evaluation demonstrated that DHA therapy considerably upregulated the expression of the pro-apoptotic MCL-1S protein. In distinction, no vital distinction within the expression of the anti-apoptotic MCL-1L protein was noticed following DHA therapy. DHA affected the expression of the apoptosis-associated protein MCL-1 via a number of mechanisms. DHA therapy elevated the ratio of MCL-1S/MCL-1L protein, thus inducing apoptosis in cholangiocarcinoma cells.
all blue protein ladder, Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.

Overexpression of p27(KIP1) induced cell cycle arrest in G(1) part and subsequent apoptosis in HCC-9204 cell line.

AIM:We’ve beforehand reported that inducible over-expression of Bak could extend cell cycle in G(1) part and result in apoptosis in HCC-9204 cells. This examine is to analyze whether or not p27(KIP1) performs an essential function on this course of.METHODS:As a way to elucidate the precise operate of p27(KIP1) on this course of, a zinc inducible p27(KIP1) secure transfectant and transient p27(KIP1)-GFP fusion transfectant had been constructed.
The results of inducible p27(KIP1) on cell development, cell cycle arrest and apoptosis had been examined within the mock, management pMD vector, and pMD-KIP1 transfected HCC-9204 cells.RESULTS:This p27(KIP1)-GFP transfectant could transiently categorical the fusion gene. The cell development was lowered by 35% at 48 h of p27(KIP1) induction with zinc therapy as decided by trypan blue exclusion assay.
These variations remained the identical after 72h of p27(KIP1) expression. p27(KIP1) precipitated cell cycle arrest after 24 h of induction, with 40% improve in G(1) inhabitants. Extended p27(KIP1) expression on this cell line induced apoptotic cell demise mirrored by TUNEL assay.

1000bp/1kb DNA ladder (blue, ready-to-use)
305-125 5x50 µg
EUR 144

Leukemia - ALL Exosome RNA
P241-ALL - Ask for price

10-250kDa Wide Range Blue-Red Two Color Protein Ladder, Prestained
BZ0010R 500ul, 500ul
EUR 200.94

10-250kDa Wide Range Blue-Red-Green Three Color Protein Ladder, Prestained
BZ0011G 500ul, 500ul
EUR 237.48

100bp DNA ladder
9K-004-0002 0.5ml
EUR 260.98

BriteRuler? Pre-stained Protein Ladder
9306-100
EUR 314.4

WSE-7020 EzProtein Ladder
2332346 1unit
EUR 339.6
Description: Pr ot ei n col or ed M ar k er

1 kb DNA Ladder
M1157-500
EUR 333.6

100 bp DNA Ladder
M1158-500
EUR 333.6

100 bp DNA Ladder
MD104-01 250 μl
EUR 132

100 bp DNA Ladder
MD104-02 500 μl
EUR 139.2

iVDye 50bp DNA Ladder
V1001-001 1ml
EUR 218.4

iVDye 50bp DNA Ladder
V1001-025 250ul
EUR 105.6

iVDye 50bp DNA Ladder
V1001-100 4x250ul
EUR 230.4

iVDye 50bp DNA Ladder
V1001-250 10X250ul
EUR 422.4

iVDye 100bp DNA Ladder
V1002-001 1ml
EUR 199.2

iVDye 100bp DNA Ladder
V1002-025 250ul
EUR 96

iVDye 100bp DNA Ladder
V1002-100 4x250ul
EUR 195.6

iVDye 100bp DNA Ladder
V1002-250 10X250ul
EUR 378

iVDye 1Kb DNA Ladder
V1003-001 1ml
EUR 148.8

iVDye 1Kb DNA Ladder
V1003-025 250ul
EUR 91.2

iVDye 1Kb DNA Ladder
V1003-100 4x250ul
EUR 157.2

iVDye 1Kb DNA Ladder
V1003-250 10x250ul
EUR 273.6

iVDye 1Kb DNA Ladder
V1003-500 20x250ul
EUR 457.2

1 kb DNA Ladder
Z6030002 100 lanes
EUR 260.4

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests 25 tests
EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Star 1kb DNA Ladder Express
BT10701 500µl
EUR 96
Description: High purity buffer for various PCR applications.

BriteRuler? 1 kb DNA Ladder
9301-100
EUR 314.4

1 Kb Ladder DNA Marker
FYD001-500UL 20 µg/ 500 µl Ask for price

100 bp Ladder DNA Marker
FYD002-500UL 30 µg/ 500 µl Ask for price

50 bp Ladder DNA Marker
FYD004-500UL 32.5 µg/ 500 µl Ask for price

Apoptotic DNA Ladder Isolation Kit
K170-50
EUR 561.6

Apoptotic DNA Ladder Isolation Kit
K2045-50 50 assays
EUR 602.4

ECOS Blue (XL1-Blue
FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE109-80VL 100 µl x 10 vials Ask for price

50 kb large-Range DNA ladder
308-025 250 µg
EUR 144

Star 100 bp DNA Ladder Express
BA01302 500µl
EUR 96
Description: High purity buffer for various PCR applications.

Wide range 1 kb DNA Ladder
M1191-500
EUR 333.6

Wide range 100 bp DNA Ladder
M1192-500
EUR 333.6

Enhanced Apoptotic DNA Ladder Detection Kit
K2202-50 50 assays
EUR 568.8

Enhanced Apoptotic DNA Ladder Detection Kit
K130-50
EUR 529.2

Quick Apoptotic DNA Ladder Detection Kit
K120-50
EUR 548.4

Quick Apoptotic DNA Ladder Detection Kit
K2194-50 50 assays
EUR 568.8

ReadiUse™ 100 bp DNA Ladder
60070 100 ul
EUR 99

ReadiUse™ 100 bp DNA Ladder
60071 2X250 ul
EUR 203

Blue Fluorescent Protein (BFP)
4994-100
EUR 451.2

Blue Fluorescent Protein (BFP)
4994-1000
EUR 2871.6

Blue Fluorescent Protein (BFP)
4994-5000
EUR 8353.2

Bromophenol Blue
CH019 25 g
EUR 158.4

Bromophenol Blue
CH020 50 g
EUR 199.2

Bromophenol Blue
CH021 100 g
EUR 272.4

Evans Blue
20-abx082082
  • EUR 210.00
  • EUR 260.40
  • 1 g
  • 5 g

Bromphenol Blue
20-abx082184
  • EUR 276.00
  • EUR 226.80
  • 25 g
  • 5 g

Evans Blue
20-abx082237
  • EUR 226.80
  • EUR 276.00
  • 1 g
  • 5 g

Bromphenol Blue
20-abx082376
  • EUR 292.80
  • EUR 226.80
  • 25 g
  • 5 g

Toluidine Blue
abx082425-5g 5 g
EUR 226.8

Trypan Blue
20-abx082476
  • EUR 276.00
  • EUR 226.80
  • 25 g
  • 5 g

Bromophenol Blue
abx082607-10g 10 g
EUR 243.6

Phthalocyanine blue
20-abx181340
  • EUR 393.60
  • EUR 260.40
  • EUR 895.20
  • 100 g
  • 25 g
  • 500 g

Bromophenol blue
abx183916-500ml 500 ml
EUR 276

Bromophenol blue
abx183916-5l 5l
EUR 710.4

Evans Blue
abx186080-125g 125g
EUR 543.6

Bromophenol blue
BB2230 25g
EUR 82.97

Bromophenol Blue
B7774-5000 5 g
EUR 104.4
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye.

Bromophenol Blue
G114 5.0 g
EUR 122.4

Evans Blue
HY-B1102 100mg
EUR 142.8

Bromothymol Blue
HY-D0012 1g
EUR 142.8

Diphenyl Blue
HY-D0970 10mM/1mL
EUR 135.6

Calcein Blue
HY-101887 25mg
EUR 142.8

Methylene Blue
HY-14536 100mg
EUR 142.8

Thiazolyl Blue
HY-15924 10mM/1mL
EUR 135.6

Meldola's blue
GT0259-10G 10 g
EUR 103.2

Meldola's blue
GT0259-25G 25 g
EUR 160.8

Meldola's blue
GT0259-50G 50 g
EUR 255.6

Bromochlorophenol Blue
GT2674-100MG 100 mg
EUR 93.6

Bromochlorophenol Blue
GT2674-1G 1 g
EUR 208.8

Bromophenol Blue
GT2874-100G 100 g
EUR 151.2

Bromophenol Blue
GT2874-10G 10 g
EUR 62.4

Bromophenol Blue
GT2874-25G 25 g
EUR 79.2

Bromophenol Blue
GT2874-50G 50 g
EUR 103.2

Bromophenol Blue
GT2874-5G 5 g
EUR 54

Hydroxynaphthol blue
GT3182-100G 100 g
EUR 284.4

Hydroxynaphthol blue
GT3182-10G 10 g
EUR 93.6

Hydroxynaphthol blue
GT3182-25G 25 g
EUR 132

Hydroxynaphthol blue
GT3182-50G 50 g
EUR 199.2

Bromothymol Blue
GT6837-10G 10 g
EUR 56.4

Bromothymol Blue
GT6837-25G 25 g
EUR 73.2

Bromothymol Blue
GT6837-50G 50 g
EUR 103.2

Bromothymol Blue
GT6837-5G 5 g
EUR 50.4

Xylenol Blue
GT7988-1G 1 g
EUR 55.2

Xylenol Blue
GT7988-25G 25 g
EUR 180

Xylenol Blue
GT7988-5G 5 g
EUR 93.6

Bromoxylenol Blue
GT8506-10G 10 g
EUR 170.4

Bromoxylenol Blue
GT8506-1G 1 g
EUR 55.2

Bromoxylenol Blue
GT8506-5G 5 g
EUR 112.8

Thymol Blue
GT9755-100G 100 g
EUR 180

Thymol Blue
GT9755-10G 10 g
EUR 60

Thymol Blue
GT9755-25G 25 g
EUR 84

Thymol Blue
GT9755-50G 50 g
EUR 112.8

Thymol Blue
GT9755-5G 5 g
EUR 50.4

Trypan blue
TT1140 10g
EUR 78.79

10-240kDa Wide Range 3 Color Prestained Protein Ladder
BG00364 500ul, 500ul
EUR 237.48

20 bp TinyOrange DNA Ladder (105ng/µl)
309-005 500 µl
EUR 129.6

20 bp TinyOrange DNA Ladder (105ng/µl)
309-025 5x500µl
EUR 387.6

Ready-to-Use 1 KB DNA Ladder
31022 1kit
EUR 160.8
Description: Minimum order quantity: 1 unit of 1kit

Ready-to-Use 100 BP DNA Ladder
31032 1kit
EUR 160.8
Description: Minimum order quantity: 1 unit of 1kit

1 kb DNA Ladder in TE buffer
31039 300uL
EUR 169.2
Description: Minimum order quantity: 1 unit of 300uL

100 bp DNA Ladder in TE buffer
31040 300uL
EUR 169.2
Description: Minimum order quantity: 1 unit of 300uL

1000bp/1kb DNA ladder (no loading dye)
305-005 50µg/250µl
EUR 60

1000bp/1kb DNA ladder (no loading dye)
305-025 5x50µg
EUR 144

1Kb Plus DNA Ladder, Ready-to-use
9K-004-0001 0.5ml
EUR 193.98

Azura PureView 250bp DNA Ladder - 100 Lanes
AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes
AZ-1125 500 Lanes
EUR 277.2

Azura PureView 250bp DNA Ladder - 1000 Lanes
AZ-1125-2 1000 Lanes
EUR 326.4

Azura PureView 100bp DNA Ladder - 100 Lanes
AZ-1131 100 Lanes
EUR 140.4

Azura PureView 100bp DNA Ladder - 500 Lanes
AZ-1135 500 Lanes
EUR 277.2

Azura PureView 100bp DNA Ladder - 1000 Lanes
AZ-1135-2 1000 Lanes
EUR 326.4

Azura PureView 50bp DNA Ladder - 100 Lanes
AZ-1151 100 Lanes
EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes
AZ-1155 500 Lanes
EUR 277.2

Azura PureView 50bp DNA Ladder - 1000 Lanes
AZ-1155-2 1000 Lanes
EUR 326.4

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

100 bp DNA Ladder 500ul per k
AXY1014 EACH
EUR 145.92

1 kb DNA Ladder 500ul per kit
AXY1016 EACH
EUR 136.8

ReadiUse™ 1 Kb Plus DNA Ladder
60050 100 ul
EUR 99

ReadiUse™ 1 Kb Plus DNA Ladder
60051 2X250 ul
EUR 203

Container 125ml PP Blue+Label+Blue Cap
CON1013 PK380
EUR 208.64

Snap Cap 11mm Blue Si/ PTFE Blue
CHR3550 PK1000
EUR 1045.38

Snap Cap 11mm Blue Si/PTFE Blue
CHR3552 PK1000
EUR 436.62

Blue Fluorescent Protein (BFP) Antibody
abx412394-50ug 50 ug
EUR 594

Blue Fluorescent protein (His tag)
80R-2547 100 ug
EUR 603.6
Description: Purified recombinant Human Blue Fluorescent protein

Protein Loading Buffer Blue 2X
NAT1250 10X1ML
EUR 52.33

Embedding Cassettes
M480-6-BLUE 1 ea.
EUR 232.8

Cassettes in QuickLoad Sleeves
M480-6SL-BLUE 1 ea.
EUR 340.8

Biometric pre-stained protein ladder (5-245 kDa) for Western
BPL11-500 500 ul
EUR 196.8

GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD
310010 1x500µl
EUR 159.6

GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD
310011 5x500µl
EUR 613.2

Container 40ml PP Blue+Label+Blue Cap Select
CON1025 PK1000
EUR 220.43

DNASE & TOLUIDINE BLUE
D04-103-10kg 10 kg
EUR 11338.8

DNASE & TOLUIDINE BLUE
D04-103-2Kg 2 Kg
EUR 2511.6

DNASE & TOLUIDINE BLUE
D04-103-500g 500 g
EUR 726

Blue Gravity Column
BSP089 1Column, 1 UNIT
EUR 77.75

Brilliant Blue G
C5579-100000 100 g
EUR 162
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility.

Thiolite™ Blue
21507 5 mg
EUR 158.4

Amplite™ Blue
11005 25 mg
EUR 211.2

CytoTell™ Blue
22251 500 Tests
EUR 158.4

CytoTell™ Blue
22252 1000 Tests
EUR 211.2

LysoBrite™ Blue
22642 500 Tests
EUR 211.2

Evans Blue Tetrasodium
2586-1G
EUR 151.2

Trypan Blue (0.4%)
1209-10
EUR 157.2

Dextran blue 2000
20-abx082076
  • EUR 260.40
  • EUR 427.20
  • 1 g
  • 5 g

Alcian Blue 8GX
abx082097-1g 1 g
EUR 260.4

Alcian Blue 8GX
abx082417-1g 1 g
EUR 243.6

Aniline Blue Solution
ABP999 1000 ml
EUR 159.6

Alkali blue 6B
AB0016 25g
EUR 80.88

Aniline Blue Solution
ABP125 125 ml
EUR 86.4

Aniline Blue Solution
ABP500 500 ml
EUR 127.2

C.I.Var Blue 6
abx180699-25g 25 g
EUR 661.2

C.I.Reactive Blue 19
20-abx181246
  • EUR 610.80
  • EUR 326.40
  • 25 g
  • 5 g

C.I.Solvent Blue 36
20-abx181247
  • EUR 510.00
  • EUR 343.20
  • EUR 243.60
  • 100 g
  • 25 g
  • 5 g

Pigment Blue 29
abx185539-100g 100 g
EUR 444

C.I.Food Blue 1
20-abx186397
  • EUR 260.40
  • EUR 678.00
  • EUR 226.80
  • EUR 460.80
  • EUR 210.00
  • 100 g
  • 1 kg
  • 25 g
  • 500 g
  • 5 g
Fourty-eight h and 72 h of p27(KIP1) expression confirmed a attribute DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak could induce cell cycle arrest in G(1) part via upregulating expression of p27(KIP1) and subsequently result in apoptosis in HCC-9204 cells. The p27(KIP1) -GFP fusion protein may be transiently expre-ssed in HCC-9204 cells. The inducible p27(KIP1)-expressing cell line gives a mannequin to evaluate p27(KIP1) operate.
Tags:
tube ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) as Tools to Explore Ubiquitin-Proteasome System and PROTAC Drug Discovery
gene tex, The biology, function, and applications of exosomes in cancer

Leave a Reply

Your email address will not be published. Required fields are marked *