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Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.
Lieven
- 0
The biopolymer chitosan is at present in widespread use due to its nontoxicity, biocompatibility and biodegradability. Subsequently, on this examine, chitosan extracted from shrimp shells was used to synthesise biogenic silver nanoparticles (AgNPs). UV-visible spectrophotometry of lowered silver nanoparticles within the colloidal answer confirmed a single peak at 400 nm, confirming the formation of AgNPs.
The presence of biomolecules liable for lowering and capping the biogenic AgNPs was confirmed by FTIR. Floor morphology of the biosynthesised AgNPs was characterised utilizing SEM, and TEM evaluation confirmed the formation of spherical shapes 17-50 nm. The presence of elemental silver within the synthesised biogenic AgNPs was confirmed utilizing EDX and the crystalline construction characterised by XRD.
Cytotoxicity of biogenic AgNPs was decided utilizing MTT and the trypan blue exclusion assay. Morphological adjustments in HepG2 cells had been detected by evaluation of the DNA ladder sample by way of gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase three and 9 protein expression outcomes confirmed cell demise by way of apoptosis. In conclusion, chitosan has the flexibility to synthesise AgNPs with in vitro apoptotic actions.
Myricetin enhances on apoptosis induced by serum deprivation in PC12 cells mediated by mitochondrial signaling pathway.
Polyphenols have many useful results and an efficient illness therapeutic auxiliary drug. Beforehand, myricetin, a polyphenol, had been reported to own numerous organic results on human physiology. Nevertheless, mechanism of myricetin on apoptosis induced in PC12 cells remains to be unclear. PC12 cells had been handled with myricetin in two focus ranges comprising 0.1 and 1 μM beneath serum-free situation. Because of this, morphological adjustments had been noticed utilizing trypan blue assay.
DNA fragmentation was decided by DNA ladder assay to judge DNA harm ranges. Western blotting outcomes confirmed that cytosolic cytochrome c which was launched from mitochondria. Subsequently, tumor suppressor gene p53, pro-apoptotic and anti-apoptotic Bcl-2 household proteins Bax and Bcl-2 had been expressed. The caspase cascade response was induced via caspase three and 9 expression. From these outcomes, it’s advised that myricetin considerably enhanced the apoptosis induced by serum deprivation in a dose-dependent method in PC12 cells.
Mechanisms of chromium hexavalent-induced apoptosis in rat testes.
Hexavalent chromium (CrVI)-containing compounds, current in industrial settings and within the setting, are often known as carcinogens and mutagens. The current examine is designed to check the speculation that oxidative stress mediates CrVI-induced apoptosis in testis. Male Wistar rats obtained an intraperitoneal injection of potassium dichromate at doses of 1 and a pair of mg kg-1.
Superoxide anion manufacturing was assessed by the willpower of the discount of cytochrome c and iodonitrotetrazolium, lipid peroxidation (LPO), metallothioneins (MTs), and catalase (CAT) exercise. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis was detected by toluidine blue staining.
The expression of Bax and Bcl-2 proteins (Pts) was additionally investigated. After 15 days of therapy, a rise of LPO and MT ranges occurred, whereas CAT exercise was decreased. Testicular tissues of handled rats confirmed pronounced degradation of the DNA into oligonucleotides as seen within the typical electrophoretic DNA ladder sample. Intense apoptosis was noticed in germinal cells of Cr-exposed rats.
Bax Pt expression was induced in spermatogonia and spermatocytes cells of CrVI-treated rats. In distinction, Bcl-2 Pt was sometimes noticed in germ cells of CrVI-exposed rats. These outcomes clearly recommend that CrVI subacute therapy causes oxidative stress in rat testis resulting in apoptosis.
Upregulation of the MCL-1S protein variant following dihydroartemisinin therapy induces apoptosis in cholangiocarcinoma cells.
The intention of the current examine was to find out whether or not dihydroartemisinin (DHA) induces apoptosis within the human cholangiocarcinoma QBC939 cell line via the regulation of myeloid cell leukemia-1 (MCL-1) expression. The inhibitory charges of DHA on QBC939 cell proliferation and the consequences of DHA on the cell demise charges at numerous DHA concentrations and following numerous therapy instances had been examined.
The speed of apoptosis and cell cycle adjustments following DHA therapy had been examined and the adjustments within the expression of MCL-1 mRNAs and MCL-1 proteins following DHA therapy had been additionally examined. The MTT assay and trypan blue staining demonstrated that DHA considerably inhibited the proliferation (P<0.05) and promoted the demise of QBC939 cells (P<0.05).
The DNA ladder assay and circulate cytometry (FCM) evaluation demonstrated that the speed of apoptosis within the experimental group was considerably elevated following DHA therapy (P<0.01). FCM evaluation additionally demonstrated that DHA therapy led to a discount within the share of QBC939 cells within the G0/G1 and G2/M phases, and the vast majority of the DNA-treated cells had been arrested within the S part of the cell cycle (P<0.01).
Western blot evaluation demonstrated that DHA therapy considerably upregulated the expression of the pro-apoptotic MCL-1S protein. In distinction, no vital distinction within the expression of the anti-apoptotic MCL-1L protein was noticed following DHA therapy. DHA affected the expression of the apoptosis-associated protein MCL-1 via a number of mechanisms. DHA therapy elevated the ratio of MCL-1S/MCL-1L protein, thus inducing apoptosis in cholangiocarcinoma cells.

Overexpression of p27(KIP1) induced cell cycle arrest in G(1) part and subsequent apoptosis in HCC-9204 cell line.
AIM:We’ve beforehand reported that inducible over-expression of Bak could extend cell cycle in G(1) part and result in apoptosis in HCC-9204 cells. This examine is to analyze whether or not p27(KIP1) performs an essential function on this course of.METHODS:As a way to elucidate the precise operate of p27(KIP1) on this course of, a zinc inducible p27(KIP1) secure transfectant and transient p27(KIP1)-GFP fusion transfectant had been constructed.
The results of inducible p27(KIP1) on cell development, cell cycle arrest and apoptosis had been examined within the mock, management pMD vector, and pMD-KIP1 transfected HCC-9204 cells.RESULTS:This p27(KIP1)-GFP transfectant could transiently categorical the fusion gene. The cell development was lowered by 35% at 48 h of p27(KIP1) induction with zinc therapy as decided by trypan blue exclusion assay.
These variations remained the identical after 72h of p27(KIP1) expression. p27(KIP1) precipitated cell cycle arrest after 24 h of induction, with 40% improve in G(1) inhabitants. Extended p27(KIP1) expression on this cell line induced apoptotic cell demise mirrored by TUNEL assay.
BlueRAY Prestained Protein Ladder |
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MBS402056-5x05mL | MyBiosource | 5x0.5mL | EUR 515 |
BlueAQUA Prestained Protein Ladder |
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MBS402059-0025mL | MyBiosource | 0.025mL | EUR 165 |
BlueAQUA Prestained Protein Ladder |
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MBS402059-05mL | MyBiosource | 0.5mL | EUR 240 |
BlueAQUA Prestained Protein Ladder |
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MBS402059-5x05mL | MyBiosource | 5x0.5mL | EUR 515 |
1000bp/1kb DNA ladder (blue, ready-to-use) |
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305-105 | GeneOn | 50µg/500µl | EUR 29 |
1000bp/1kb DNA ladder (blue, ready-to-use) |
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305-105L | GeneOn | 10x50 µg | EUR 199 |
1000bp/1kb DNA ladder (blue, ready-to-use) |
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305-105XL | GeneOn | 20x50 µg | EUR 379 |
1000bp/1kb DNA ladder (blue, ready-to-use) |
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305-125 | GeneOn | 5x50 µg | EUR 109 |
100bp plus DNA ladder (blue, ready-to-use) |
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304-105 | GeneOn | 50µg/500µl | EUR 29 |
100bp plus DNA ladder (blue, ready-to-use) |
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304-105L | GeneOn | 10x50µg | EUR 199 |
100bp plus DNA ladder (blue, ready-to-use) |
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304-105XL | GeneOn | 20x50µg | EUR 379 |
100bp plus DNA ladder (blue, ready-to-use) |
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304-125 | GeneOn | 5x50 µg | EUR 109 |
10-250kDa Wide Range Blue-Red-Green Three Color Protein Ladder, Prestained |
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BZ0011G | Bio Basic | 500ul, 500ul | EUR 237.48 |
BLUltra Prestained Protein Ladder |
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MBS402055-0025mL | MyBiosource | 0.025mL | EUR 165 |
BLUltra Prestained Protein Ladder |
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MBS402055-05mL | MyBiosource | 0.5mL | EUR 240 |
BLUltra Prestained Protein Ladder |
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MBS402055-5x05mL | MyBiosource | 5x0.5mL | EUR 515 |
PRESTAINED PROTEIN LADDER |
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IB01210 | IBI Scientific | 245kDa | EUR 258.78 |
Prestained Protein Ladder 245 kDa |
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PL0245 | Geneaid Biotech | each | EUR 62 |
Prestained Protein Ladder 245 kDa |
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PL0245S | Geneaid Biotech | each | EUR 2.2 |
Prestained Protein Ladder (100 Lanes) |
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MBT092-100LN | EWC Diagnostics | 1 unit | EUR 108.83 |
Description: Prestained Protein Ladder (100 Lanes) |
Prestained Protein Ladder V |
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PL005 | Geneaid Biotech | each | EUR 50 |
Prestained Protein Ladder V |
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PL005S | Geneaid Biotech | each | EUR 2.2 |
Prestained Color Protein Ladder |
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PSC1065 | Creative BioMart | 250ul | EUR 63.2 |
Description: Store at 4 centigrade for 2 months. |
UNveil Unstained Protein Ladder |
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MBS402060-0025mL | MyBiosource | 0.025mL | EUR 165 |
UNveil Unstained Protein Ladder |
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MBS402060-05mL | MyBiosource | 0.5mL | EUR 220 |
UNveil Unstained Protein Ladder |
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MBS402060-5x05mL | MyBiosource | 5x0.5mL | EUR 410 |
Protein MW Marker, Ladder, 8.5-70kD |
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MBS657643-05mL | MyBiosource | 0.5mL | EUR 455 |
Protein MW Marker, Ladder, 8.5-70kD |
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MBS657643-5x05mL | MyBiosource | 5x0.5mL | EUR 1890 |
Prestained Protein Ladder (10 Lanes) |
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MBT092-10LN | EWC Diagnostics | 1 unit | EUR 13.13 |
Description: Prestained Protein Ladder (10 Lanes) |
ProLiteâ„¢ 5-245 kD Protein Ladder |
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18015 | AAT Bioquest | 100uL | EUR 97 |
Description: The ProLiteâ„¢ 5-245 kD Protein Ladder is a high-quality three-color protein standard consisting of 13 prestained proteins. |
Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
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PMB10-0500 | Bio-Helix | 500 μl | EUR 55 |
Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
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PMB12-0500 | Bio-Helix | 500 μl | EUR 60 |
Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
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PMB13-0500 | Bio-Helix | 500 μl | EUR 66 |
BriteRuler? Pre-stained Protein Ladder |
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9306-100 | Biovision | each | EUR 314.4 |
Tri-Color Protein Ladder, 12 bands |
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MBS433532-025mL | MyBiosource | 0.25mL | EUR 200 |
Tri-Color Protein Ladder, 12 bands |
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MBS433532-5x025mL | MyBiosource | 5x0.25mL | EUR 755 |
Perfect™M Tricolor Protein Ladder |
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E3210-01 | EURx | 50loadings | EUR 66.49 |
Description: Prestained protein ladder for sizing polypeptides ranging from 11 kDa to 245 kDa(11, 17, 20, 25, 35, 48, 63, 75, 100, 135, 180, 245 kDa) |
Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
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PMB01-0500 | Bio-Helix | 500 μl | EUR 55 |
EzWay Protein PreBlue Ladder, 11 bands |
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K18010 | KOMA BIOTECH | 500ul | EUR 108 |
Description: https://komabiotech.co.kr/labiskoma/datasheet/K18010.pdf |
TriColor Protein Ladder 10-180 kDa (100 minigel lanes) |
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BRBR0900201 | Westburg | each | EUR 138.98 |
Tri-Color Protein Ladder II, 10 bands |
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MBS433529-025mL | MyBiosource | 0.25mL | EUR 200 |
Tri-Color Protein Ladder II, 10 bands |
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MBS433529-5x025mL | MyBiosource | 5x0.25mL | EUR 755 |
EzWay Protein Multicolor Ladder, 10 bands |
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K18020 | KOMA BIOTECH | 500ul | EUR 122 |
Description: https://komabiotech.co.kr/labiskoma/datasheet/K18020.pdf |
Tricolor Broad Range Prestained Protein Ladder, 2 x 250µl |
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PR0624 | Vivantis | each | Ask for price |
Blu11 Prestained Protein Ladder (Single color, 11 pre-stained bands, 10-180 kDa) |
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PMB11-0500 | Bio-Helix | 500 μl | EUR 30 |
TriColor Broad Protein Ladder 3.5-245 kDa (100 minigel lanes) |
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BRBR0900101 | Westburg | each | EUR 152.6 |
Protein MW Marker, Ladder, Prestained, 10-170kD |
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MBS657624-025mL | MyBiosource | 0.25mL | EUR 505 |
Protein MW Marker, Ladder, Prestained, 10-170kD |
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MBS657624-5x025mL | MyBiosource | 5x0.25mL | EUR 2130 |
PCR Ladder |
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77305 | Sisco Laboratories | 10 mcg | EUR 15.91 |
Description: Part E |
PCR Ladder |
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77305-1 | Sisco Laboratories | 50 mcg | EUR 50.55 |
Description: Part E |
1kb Ladder |
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MBS278040-100Reactions | MyBiosource | 100Reactions | EUR 150 |
DNA LADDER (100bp) |
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IB01301 | IBI Scientific | 50uG/500uL | EUR 59.65 |
Description: [FR] |
DNA LADDER (100bp) |
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IB01302 | IBI Scientific | 50uG/500uL | EUR 56.1 |
Description: (NO LOADING DYE) [FR] |
Biometric pre-stained protein ladder (5-245 kDa) for Western |
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BPL11-500 | Alpha Diagnostics | 500 ul | EUR 196.8 |
100bp Ladder |
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MBS278039-100Reactions | MyBiosource | 100Reactions | EUR 150 |
PreStained High Range 2 Tri-Coloured Protein Ladder: 10-245kDa |
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67792 | Sisco Laboratories | 0.25 ml | EUR 97.93 |
Description: Part E |
PreStained High Range 2 Tri-Coloured Protein Ladder: 10-245kDa |
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67792-1 | Sisco Laboratories | 1 ml | EUR 369.64 |
Description: Part E |
PreStained High Range 1 Tri-Coloured Protein Ladder: 10-180kDa |
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27839 | Sisco Laboratories | 0.25 ml | EUR 103.91 |
Description: Part E |
PreStained High Range 1 Tri-Coloured Protein Ladder: 10-180kDa |
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27839-1 | Sisco Laboratories | 1 ml | EUR 299.77 |
Description: Part E |
Protein Ladder PS11 prestained, 11 bands, 10 - 175kD |
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310005 | GeneOn | 1x500µl | EUR 99 |
Protein Ladder PS11 prestained, 11 bands, 10 - 175kD |
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310006 | GeneOn | 5x500µl | EUR 399 |
ProSignal® Full-Range Prestained Protein Ladder |
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83-650 | Genesee Scientific | 500ul/Unit | EUR 60.25 |
Description: 3 Color, 10 to 245 kDa |
ProSignal® Full-Range Prestained Protein Ladder |
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83-660 | Genesee Scientific | 500ul/Unit | EUR 80.25 |
Description: 3 Color, 10 to 250 kDa |
Protein Ladder Extended prestained, 13 bands, 5 - 245kD |
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310010 | GeneOn | 1x500µl | EUR 109 |
Protein Ladder Extended prestained, 13 bands, 5 - 245kD |
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310011 | GeneOn | 5x500µl | EUR 520 |
1 kb Ladder |
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MBS433527-005mg | MyBiosource | 0.05mg | EUR 160 |
1 kb Ladder |
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MBS433527-5x005mg | MyBiosource | 5x0.05mg | EUR 580 |
VC1kb DNA Ladder, 50µg |
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NL1409 | Vivantis | each | Ask for price |
1kb RNA Ladder |
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15003 | Norgen Biotek Corp | 50 Loads | EUR 89.4 |
DNA LADDER (1KB) |
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IB01351 | IBI Scientific | 50uG/500uL | EUR 61.65 |
Description: [FR] |
Protein Ladder PS10 PLUS prestained, 10 bands, 11 - 180kD |
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310003 | GeneOn | 1x500 µl | EUR 99 |
Protein Ladder PS10 PLUS prestained, 10 bands, 11 - 180kD |
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310004 | GeneOn | 5x500µl | EUR 399 |
100b RNA Ladder |
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15002 | Norgen Biotek Corp | 50 Loads | EUR 65.4 |
Protein Ladder Wide Range prestained, 10 bands, 6,5 - 270kD |
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310020 | GeneOn | 1x500µl | EUR 125 |
Protein Ladder Wide Range prestained, 10 bands, 6,5 - 270kD |
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310021 | GeneOn | 5x500µl | EUR 499 |
Protein Ladder One Plus, Triple-color for SDS-PAGE |
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19593-25 | NACALAI TESQUE | 500UL | EUR 136.5 |
VC100bp DNA Ladder, 50µg |
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NL1401 | Vivantis | each | Ask for price |
100bp DNA ladder |
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9K-004-0002 | Bio Basic | 0.5ml | EUR 260.98 |
100bp DNA ladder |
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TBS4030-0.5mL | Tribioscience | 0.5 ml | EUR 60 |
100bp DNA ladder |
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TBS4030-1.0mL | Tribioscience | 1.0 ml | EUR 110 |
10-240kDa Wide Range 3 Color Prestained Protein Ladder |
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BG00364 | Bio Basic | 500ul, 500ul | EUR 237.48 |
SafeDye PCR Ladder |
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64935 | Sisco Laboratories | 50 mcg | EUR 88.69 |
Description: Part E |
SafeDye 100bp Ladder |
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33902 | Sisco Laboratories | 50 mcg | EUR 92.28 |
Description: Part E |
SafeDye 500bp Ladder |
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27107 | Sisco Laboratories | 50 mcg | EUR 110.24 |
Description: Part E |
ProxiO 1kb DNA Ladder |
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48290 | Sisco Laboratories | 50 mcg | EUR 47.55 |
Description: Part E |
ProxiO 1kb DNA Ladder |
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48290-1 | Sisco Laboratories | 5 x50 mcg | EUR 203.47 |
Description: Part E |
ProxiO Low DNA Ladder |
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68905 | Sisco Laboratories | 50 mcg | EUR 64.14 |
Description: Part E |
ProxiO Low DNA Ladder |
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68905-1 | Sisco Laboratories | 5 x50 mcg | EUR 314.48 |
Description: Part E |
Un-stained protein ladder (14-116 kDa) for mol wt determination by Western |
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PMU11-500 | Alpha Diagnostics | 50 loads | EUR 110.4 |
ProxiO 10bp DNA Ladder |
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44897 | Sisco Laboratories | 50 mcg | EUR 84.93 |
Description: Part E |
ProxiB 100bp DNA Ladder |
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84628 | Sisco Laboratories | 50 mcg | EUR 48.58 |
Description: Part E |
ProxiB 100bp DNA Ladder |
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84628-1 | Sisco Laboratories | 5 x50 mcg | EUR 188.07 |
Description: Part E |
ProxiO 20bp DNA Ladder |
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98628 | Sisco Laboratories | 50 mcg | EUR 76.2 |
Description: Part E |
ProxiO 100bp DNA Ladder |
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92972 | Sisco Laboratories | 50 mcg | EUR 48.58 |
Description: Part E |
ProxiO 100bp DNA Ladder |
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92972-1 | Sisco Laboratories | 5 x50 mcg | EUR 188.07 |
Description: Part E |
ProxiO 500bp DNA Ladder |
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93121 | Sisco Laboratories | 50 mcg | EUR 78.09 |
Description: Part E |
ProxiO 200bp DNA Ladder |
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37217 | Sisco Laboratories | 50 mcg | EUR 74.24 |
Description: Part E |
ProxiO 50bp DNA Ladder |
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82208 | Sisco Laboratories | 50 mcg | EUR 60.47 |
Description: Part E |
ProxiO 50bp DNA Ladder |
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82208-1 | Sisco Laboratories | 5 x50 mcg | EUR 198.76 |
Description: Part E |
VC 50bp DNA Ladder, 50µg |
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NL1421 | Vivantis | each | Ask for price |
FastRunner DNA Ladder |
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12800 | Norgen Biotek Corp | 100 Loads | EUR 45 |
FastRunner DNA Ladder |
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12815 | Norgen Biotek Corp | 5 x 100 Loads | EUR 215.4 |
50 bp DNA Ladder |
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13525 | Norgen Biotek Corp | 1 Unit | EUR 45 |
50 bp DNA Ladder |
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13527 | Norgen Biotek Corp | 5 x 100 Loads | EUR 215.4 |
1 Kb DNA Ladder |
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DL006 | Geneaid Biotech | each | EUR 17.3 |
1 Kb DNA Ladder |
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DL006S | Geneaid Biotech | each | EUR 1.2 |
50 bp DNA Ladder |
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DL008 | Geneaid Biotech | each | EUR 45 |
50 bp DNA Ladder |
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DL008S | Geneaid Biotech | each | EUR 2.2 |
20 bp DNA Ladder |
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M-212L | Jena Bioscience GmbH | 5 x 500µl | EUR 261.58 |
Description: ready-to-use, orange |
20 bp DNA Ladder |
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M-212S | Jena Bioscience GmbH | 500µl | EUR 65.47 |
Description: ready-to-use, orange |
50 bp DNA Ladder |
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M-213L | Jena Bioscience GmbH | 5 x 500µl | EUR 218.03 |
Description: ready-to-use, orange |
50 bp DNA Ladder |
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M-213S | Jena Bioscience GmbH | 500µl | EUR 54.51 |
Description: ready-to-use, orange |
1 kb DNA Ladder |
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M-217L | Jena Bioscience GmbH | 5 x 500µl | EUR 130.64 |
Description: ready-to-use, blue / blue |
1 kb DNA Ladder |
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M-217S | Jena Bioscience GmbH | 500µl | EUR 32.66 |
Description: ready-to-use, blue / blue |
1 kb DNA Ladder |
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M1157-500 | Biovision | each | EUR 333.6 |
50 bp DNA Ladder |
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MBS433533-005mg | MyBiosource | 0.05mg | EUR 215 |
50 bp DNA Ladder |
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MBS433533-5x005mg | MyBiosource | 5x0.05mg | EUR 815 |
1 kb DNA Ladder |
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MBS9143981-05mL | MyBiosource | 0.5mL | EUR 200 |
1 kb DNA Ladder |
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MBS9143981-25mL | MyBiosource | 2.5mL | EUR 450 |
1 kb DNA Ladder |
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MBS9143981-5x25mL | MyBiosource | 5x2.5mL | EUR 1840 |
1 kb DNA Ladder |
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RM19005 | Abclonal | 500μL | EUR 28.89 |
1 kb DNA Ladder |
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Z6030002 | Biochain | 100 lanes | EUR 158 |
FluroBronze PCR Ladder |
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76195 | Sisco Laboratories | 50 mcg | EUR 94.68 |
Description: Part E |
100 bp DNA Ladder |
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DL007 | Geneaid Biotech | each | EUR 17.3 |
100 bp DNA Ladder |
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DL007S | Geneaid Biotech | each | EUR 1.2 |
100 bp DNA Ladder |
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M-214L | Jena Bioscience GmbH | 5 x 500µl | EUR 181.74 |
Description: ready-to-use, orange / blue |
100 bp DNA Ladder |
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M-214S | Jena Bioscience GmbH | 500µl | EUR 45.44 |
Description: ready-to-use, orange / blue |
200 bp DNA Ladder |
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M-215L | Jena Bioscience GmbH | 5 x 500µl | EUR 174.41 |
Description: ready-to-use, blue / blue |
200 bp DNA Ladder |
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M-215S | Jena Bioscience GmbH | 500µl | EUR 43.55 |
Description: ready-to-use, blue / blue |
500 bp DNA Ladder |
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M-216L | Jena Bioscience GmbH | 5 x 500µl | EUR 130.64 |
Description: ready-to-use, blue / blue |
500 bp DNA Ladder |
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M-216S | Jena Bioscience GmbH | 500µl | EUR 32.66 |
Description: ready-to-use, blue / blue |
100 bp DNA Ladder |
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M1158-500 | Biovision | each | EUR 333.6 |
100 bp DNA Ladder |
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MBS433528-005mg | MyBiosource | 0.05mg | EUR 175 |
100 bp DNA Ladder |
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MBS433528-5x005mg | MyBiosource | 5x0.05mg | EUR 640 |
100 bp DNA Ladder |
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MD104-01 | Vazyme | 250 μl | EUR 18.64 |
100 bp DNA Ladder |
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MD104-01-50rxns | Vazyme | 50 rxns | EUR 19.3 |
100 bp DNA Ladder |
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MD104-02 | Vazyme | 500 μl | EUR 29 |
100 bp DNA Ladder |
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MD104-02-100rxns | Vazyme | 100 rxns | EUR 30.03 |
100 bp DNA Ladder |
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MBS9143983-05mL | MyBiosource | 0.5mL | EUR 165 |
100 bp DNA Ladder |
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MBS9143983-25mL | MyBiosource | 2.5mL | EUR 340 |
100 bp DNA Ladder |
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MBS9143983-5x25mL | MyBiosource | 5x2.5mL | EUR 1345 |
100 bp DNA Ladder |
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RK30193 | Abclonal | 500μL | EUR 130.8 |
100 bp DNA Ladder |
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RM19007 | Abclonal | 500μL | EUR 32.16 |
FluroBronze 500bp Ladder |
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54896 | Sisco Laboratories | 50 mcg | EUR 122.22 |
Description: Part E |
FluroBronze 100bp Ladder |
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97958 | Sisco Laboratories | 50 mcg | EUR 92.28 |
Description: Part E |
100bp DNA ladder (200 Lanes) |
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MBT049-200LN | EWC Diagnostics | 1 unit | EUR 142.29 |
Description: 100bp DNA ladder (200 Lanes) |
Apex DNA Ladder I |
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42-427 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 295.59 |
Description: 200 Lanes, 200-12,000bp |
Apex DNA Ladder I |
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42-428 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 463.43 |
Description: 500 Lanes, 200-12,000bp |
Apex DNA Ladder II |
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42-429 | Genesee Scientific | 1 x 0.5ml/Unit | EUR 90.91 |
Description: 100 Lanes, 100-1,000bp |
Apex DNA Ladder II |
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42-430 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 181.82 |
Description: 200 Lanes, 100-1,000bp |
Apex DNA Ladder II |
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42-431 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 454.32 |
Description: 500 Lanes, 100-1,000bp |
Apex DNA Ladder III |
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42-424 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 187.26 |
Description: 200 Lanes, 80bp - 10Kb |
Apex DNA Ladder III |
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42-425 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 467.98 |
Description: 500 Lanes, 80bp - 10Kb |
Apex PCR DNA Ladder |
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42-432 | Genesee Scientific | 1 x 0.5ml/Unit | EUR 72.48 |
Description: 100 Lanes, 100-3,000bp |
Apex PCR DNA Ladder |
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42-433 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 144.97 |
Description: 200 Lanes, 100-3,000bp |
Apex PCR DNA Ladder |
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42-434 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 362.3 |
Description: 500 Lanes, 100-3,000bp |
VC100bp Plus DNA Ladder, 50µg |
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NL1405 | Vivantis | each | Ask for price |
Apex 500bp DNA Ladder |
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19-113 | Genesee Scientific | 1ml/Unit | EUR 132.31 |
Description: 200 Gel Lanes, 500bp - 5kb |
Low Range DNA Ladder |
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MBS136045-05mL | MyBiosource | 0.5mL | EUR 260 |
Low Range DNA Ladder |
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MBS136045-1mL | MyBiosource | 1mL | EUR 380 |
Low Range DNA Ladder |
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MBS136045-5x1mL | MyBiosource | 5x1mL | EUR 1445 |
Mid Range DNA Ladder |
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MBS136048-05mL | MyBiosource | 0.5mL | EUR 260 |
Mid Range DNA Ladder |
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MBS136048-1mL | MyBiosource | 1mL | EUR 380 |
Mid Range DNA Ladder |
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MBS136048-5x1mL | MyBiosource | 5x1mL | EUR 1445 |
*Low Range DNA Ladder |
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IDNALDRLEL500UL | Innovative research | each | EUR 157 |
Description: *Low Range DNA Ladder |
*Mid Range DNA Ladder |
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IDNALDRMEL500UL | Innovative research | each | EUR 135 |
Description: *Mid Range DNA Ladder |
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Fourty-eight h and 72 h of p27(KIP1) expression confirmed a attribute DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak could induce cell cycle arrest in G(1) part via upregulating expression of p27(KIP1) and subsequently result in apoptosis in HCC-9204 cells. The p27(KIP1) -GFP fusion protein may be transiently expre-ssed in HCC-9204 cells. The inducible p27(KIP1)-expressing cell line gives a mannequin to evaluate p27(KIP1) operate.
Tags: 3x flag tag 6x his tag agarose gel loading dye bam hi restriction site bl21 (de3) blasticidin invitrogen bsmbi cas9 vector cmv promoter sequence crispr pam sequence crispr vector dh5 alpha cells dsred sequence ecorv egfp clontech egfp nucleotide sequence flag tag vector g418 antibiotic gel red dna stain gibson assembly gibson kit hek293t media midiprep protocol mini prep kit miniprep dna nhe1 neb pbabe vector pbr322 plasmid map pbs sk pcdna3 0 pcdna3 2 pcr blunt ii topo pet plasmid pet101 pet28a+ pgem t easy vector pgex 4t2 pgex4t3 pgipz vector pjet vector plko.1 puro pmd2 g promoter sequence ptriex ptripz map strep ii tag t7 tag ta cloning vector tdtomato sequence virapower zsgreen