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Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.
Lieven
- 0
The biopolymer chitosan is at present in widespread use due to its nontoxicity, biocompatibility and biodegradability. Subsequently, on this examine, chitosan extracted from shrimp shells was used to synthesise biogenic silver nanoparticles (AgNPs). UV-visible spectrophotometry of lowered silver nanoparticles within the colloidal answer confirmed a single peak at 400 nm, confirming the formation of AgNPs.
The presence of biomolecules liable for lowering and capping the biogenic AgNPs was confirmed by FTIR. Floor morphology of the biosynthesised AgNPs was characterised utilizing SEM, and TEM evaluation confirmed the formation of spherical shapes 17-50 nm. The presence of elemental silver within the synthesised biogenic AgNPs was confirmed utilizing EDX and the crystalline construction characterised by XRD.
Cytotoxicity of biogenic AgNPs was decided utilizing MTT and the trypan blue exclusion assay. Morphological adjustments in HepG2 cells had been detected by evaluation of the DNA ladder sample by way of gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase three and 9 protein expression outcomes confirmed cell demise by way of apoptosis. In conclusion, chitosan has the flexibility to synthesise AgNPs with in vitro apoptotic actions.
Myricetin enhances on apoptosis induced by serum deprivation in PC12 cells mediated by mitochondrial signaling pathway.
Polyphenols have many useful results and an efficient illness therapeutic auxiliary drug. Beforehand, myricetin, a polyphenol, had been reported to own numerous organic results on human physiology. Nevertheless, mechanism of myricetin on apoptosis induced in PC12 cells remains to be unclear. PC12 cells had been handled with myricetin in two focus ranges comprising 0.1 and 1 μM beneath serum-free situation. Because of this, morphological adjustments had been noticed utilizing trypan blue assay.
DNA fragmentation was decided by DNA ladder assay to judge DNA harm ranges. Western blotting outcomes confirmed that cytosolic cytochrome c which was launched from mitochondria. Subsequently, tumor suppressor gene p53, pro-apoptotic and anti-apoptotic Bcl-2 household proteins Bax and Bcl-2 had been expressed. The caspase cascade response was induced via caspase three and 9 expression. From these outcomes, it’s advised that myricetin considerably enhanced the apoptosis induced by serum deprivation in a dose-dependent method in PC12 cells.
Mechanisms of chromium hexavalent-induced apoptosis in rat testes.
Hexavalent chromium (CrVI)-containing compounds, current in industrial settings and within the setting, are often known as carcinogens and mutagens. The current examine is designed to check the speculation that oxidative stress mediates CrVI-induced apoptosis in testis. Male Wistar rats obtained an intraperitoneal injection of potassium dichromate at doses of 1 and a pair of mg kg-1.
Superoxide anion manufacturing was assessed by the willpower of the discount of cytochrome c and iodonitrotetrazolium, lipid peroxidation (LPO), metallothioneins (MTs), and catalase (CAT) exercise. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis was detected by toluidine blue staining.
The expression of Bax and Bcl-2 proteins (Pts) was additionally investigated. After 15 days of therapy, a rise of LPO and MT ranges occurred, whereas CAT exercise was decreased. Testicular tissues of handled rats confirmed pronounced degradation of the DNA into oligonucleotides as seen within the typical electrophoretic DNA ladder sample. Intense apoptosis was noticed in germinal cells of Cr-exposed rats.
Bax Pt expression was induced in spermatogonia and spermatocytes cells of CrVI-treated rats. In distinction, Bcl-2 Pt was sometimes noticed in germ cells of CrVI-exposed rats. These outcomes clearly recommend that CrVI subacute therapy causes oxidative stress in rat testis resulting in apoptosis.
Upregulation of the MCL-1S protein variant following dihydroartemisinin therapy induces apoptosis in cholangiocarcinoma cells.
The intention of the current examine was to find out whether or not dihydroartemisinin (DHA) induces apoptosis within the human cholangiocarcinoma QBC939 cell line via the regulation of myeloid cell leukemia-1 (MCL-1) expression. The inhibitory charges of DHA on QBC939 cell proliferation and the consequences of DHA on the cell demise charges at numerous DHA concentrations and following numerous therapy instances had been examined.
The speed of apoptosis and cell cycle adjustments following DHA therapy had been examined and the adjustments within the expression of MCL-1 mRNAs and MCL-1 proteins following DHA therapy had been additionally examined. The MTT assay and trypan blue staining demonstrated that DHA considerably inhibited the proliferation (P<0.05) and promoted the demise of QBC939 cells (P<0.05).
The DNA ladder assay and circulate cytometry (FCM) evaluation demonstrated that the speed of apoptosis within the experimental group was considerably elevated following DHA therapy (P<0.01). FCM evaluation additionally demonstrated that DHA therapy led to a discount within the share of QBC939 cells within the G0/G1 and G2/M phases, and the vast majority of the DNA-treated cells had been arrested within the S part of the cell cycle (P<0.01).
Western blot evaluation demonstrated that DHA therapy considerably upregulated the expression of the pro-apoptotic MCL-1S protein. In distinction, no vital distinction within the expression of the anti-apoptotic MCL-1L protein was noticed following DHA therapy. DHA affected the expression of the apoptosis-associated protein MCL-1 via a number of mechanisms. DHA therapy elevated the ratio of MCL-1S/MCL-1L protein, thus inducing apoptosis in cholangiocarcinoma cells.

Overexpression of p27(KIP1) induced cell cycle arrest in G(1) part and subsequent apoptosis in HCC-9204 cell line.
AIM:We’ve beforehand reported that inducible over-expression of Bak could extend cell cycle in G(1) part and result in apoptosis in HCC-9204 cells. This examine is to analyze whether or not p27(KIP1) performs an essential function on this course of.METHODS:As a way to elucidate the precise operate of p27(KIP1) on this course of, a zinc inducible p27(KIP1) secure transfectant and transient p27(KIP1)-GFP fusion transfectant had been constructed.
The results of inducible p27(KIP1) on cell development, cell cycle arrest and apoptosis had been examined within the mock, management pMD vector, and pMD-KIP1 transfected HCC-9204 cells.RESULTS:This p27(KIP1)-GFP transfectant could transiently categorical the fusion gene. The cell development was lowered by 35% at 48 h of p27(KIP1) induction with zinc therapy as decided by trypan blue exclusion assay.
These variations remained the identical after 72h of p27(KIP1) expression. p27(KIP1) precipitated cell cycle arrest after 24 h of induction, with 40% improve in G(1) inhabitants. Extended p27(KIP1) expression on this cell line induced apoptotic cell demise mirrored by TUNEL assay.
1000bp/1kb DNA ladder (blue, ready-to-use) |
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305-125 | GeneOn | 5x50 µg | EUR 144 |
Leukemia - ALL Exosome RNA |
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P241-ALL | 101Bio | - | Ask for price |
10-250kDa Wide Range Blue-Red Two Color Protein Ladder, Prestained |
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BZ0010R | Bio Basic | 500ul, 500ul | EUR 200.94 |
10-250kDa Wide Range Blue-Red-Green Three Color Protein Ladder, Prestained |
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BZ0011G | Bio Basic | 500ul, 500ul | EUR 237.48 |
100bp DNA ladder |
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9K-004-0002 | Bio Basic | 0.5ml | EUR 260.98 |
BriteRuler? Pre-stained Protein Ladder |
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9306-100 | Biovision | EUR 314.4 |
WSE-7020 EzProtein Ladder |
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2332346 | Atto | 1unit | EUR 339.6 |
Description: Pr ot ei n col or ed M ar k er |
1 kb DNA Ladder |
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M1157-500 | Biovision | EUR 333.6 |
100 bp DNA Ladder |
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M1158-500 | Biovision | EUR 333.6 |
100 bp DNA Ladder |
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MD104-01 | Vazyme | 250 μl | EUR 132 |
100 bp DNA Ladder |
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MD104-02 | Vazyme | 500 μl | EUR 139.2 |
iVDye 50bp DNA Ladder |
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V1001-001 | GenDepot | 1ml | EUR 218.4 |
iVDye 50bp DNA Ladder |
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V1001-025 | GenDepot | 250ul | EUR 105.6 |
iVDye 50bp DNA Ladder |
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V1001-100 | GenDepot | 4x250ul | EUR 230.4 |
iVDye 50bp DNA Ladder |
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V1001-250 | GenDepot | 10X250ul | EUR 422.4 |
iVDye 100bp DNA Ladder |
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V1002-001 | GenDepot | 1ml | EUR 199.2 |
iVDye 100bp DNA Ladder |
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V1002-025 | GenDepot | 250ul | EUR 96 |
iVDye 100bp DNA Ladder |
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V1002-100 | GenDepot | 4x250ul | EUR 195.6 |
iVDye 100bp DNA Ladder |
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V1002-250 | GenDepot | 10X250ul | EUR 378 |
iVDye 1Kb DNA Ladder |
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V1003-001 | GenDepot | 1ml | EUR 148.8 |
iVDye 1Kb DNA Ladder |
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V1003-025 | GenDepot | 250ul | EUR 91.2 |
iVDye 1Kb DNA Ladder |
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V1003-100 | GenDepot | 4x250ul | EUR 157.2 |
iVDye 1Kb DNA Ladder |
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V1003-250 | GenDepot | 10x250ul | EUR 273.6 |
iVDye 1Kb DNA Ladder |
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V1003-500 | GenDepot | 20x250ul | EUR 457.2 |
1 kb DNA Ladder |
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Z6030002 | Biochain | 100 lanes | EUR 260.4 |
2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) |
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GEN-402-25tests | All test | 25 tests | EUR 292.8 |
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
Star 1kb DNA Ladder Express |
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BT10701 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
BriteRuler? 1 kb DNA Ladder |
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9301-100 | Biovision | EUR 314.4 |
1 Kb Ladder DNA Marker |
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FYD001-500UL | Yeastern Biotech | 20 µg/ 500 µl | Ask for price |
100 bp Ladder DNA Marker |
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FYD002-500UL | Yeastern Biotech | 30 µg/ 500 µl | Ask for price |
50 bp Ladder DNA Marker |
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FYD004-500UL | Yeastern Biotech | 32.5 µg/ 500 µl | Ask for price |
Apoptotic DNA Ladder Isolation Kit |
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K170-50 | Biovision | EUR 561.6 |
Apoptotic DNA Ladder Isolation Kit |
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K2045-50 | ApexBio | 50 assays | EUR 602.4 |
ECOS Blue (XL1-Blue |
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FYE107-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE107-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE108-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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ECOS Blue (XL1-Blue |
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FYE109-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE109-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
50 kb large-Range DNA ladder |
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308-025 | GeneOn | 250 µg | EUR 144 |
Star 100 bp DNA Ladder Express |
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BA01302 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
Wide range 1 kb DNA Ladder |
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M1191-500 | Biovision | EUR 333.6 |
Wide range 100 bp DNA Ladder |
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M1192-500 | Biovision | EUR 333.6 |
Enhanced Apoptotic DNA Ladder Detection Kit |
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K2202-50 | ApexBio | 50 assays | EUR 568.8 |
Enhanced Apoptotic DNA Ladder Detection Kit |
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K130-50 | Biovision | EUR 529.2 |
Quick Apoptotic DNA Ladder Detection Kit |
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K120-50 | Biovision | EUR 548.4 |
Quick Apoptotic DNA Ladder Detection Kit |
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K2194-50 | ApexBio | 50 assays | EUR 568.8 |
ReadiUse™ 100 bp DNA Ladder |
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60070 | AAT Bioquest | 100 ul | EUR 99 |
ReadiUse™ 100 bp DNA Ladder |
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60071 | AAT Bioquest | 2X250 ul | EUR 203 |
Blue Fluorescent Protein (BFP) |
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4994-100 | Biovision | EUR 451.2 |
Blue Fluorescent Protein (BFP) |
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4994-1000 | Biovision | EUR 2871.6 |
Blue Fluorescent Protein (BFP) |
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4994-5000 | Biovision | EUR 8353.2 |
Bromophenol Blue |
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CH019 | ABM | 25 g | EUR 158.4 |
Bromophenol Blue |
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CH020 | ABM | 50 g | EUR 199.2 |
Bromophenol Blue |
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CH021 | ABM | 100 g | EUR 272.4 |
Evans Blue |
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20-abx082082 | Abbexa |
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Bromphenol Blue |
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20-abx082184 | Abbexa |
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Evans Blue |
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20-abx082237 | Abbexa |
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20-abx082376 | Abbexa |
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Toluidine Blue |
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abx082425-5g | Abbexa | 5 g | EUR 226.8 |
Trypan Blue |
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20-abx082476 | Abbexa |
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Bromophenol Blue |
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abx082607-10g | Abbexa | 10 g | EUR 243.6 |
Phthalocyanine blue |
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20-abx181340 | Abbexa |
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Bromophenol blue |
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abx183916-500ml | Abbexa | 500 ml | EUR 276 |
Bromophenol blue |
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abx183916-5l | Abbexa | 5l | EUR 710.4 |
Evans Blue |
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abx186080-125g | Abbexa | 125g | EUR 543.6 |
Bromophenol blue |
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BB2230 | Bio Basic | 25g | EUR 82.97 |
Bromophenol Blue |
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B7774-5000 | ApexBio | 5 g | EUR 104.4 |
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye. |
Bromophenol Blue |
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G114 | ABM | 5.0 g | EUR 122.4 |
Evans Blue |
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HY-B1102 | MedChemExpress | 100mg | EUR 142.8 |
Bromothymol Blue |
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HY-D0012 | MedChemExpress | 1g | EUR 142.8 |
Diphenyl Blue |
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HY-D0970 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Calcein Blue |
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HY-101887 | MedChemExpress | 25mg | EUR 142.8 |
Methylene Blue |
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HY-14536 | MedChemExpress | 100mg | EUR 142.8 |
Thiazolyl Blue |
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HY-15924 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Meldola's blue |
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GT0259-10G | Glentham Life Sciences | 10 g | EUR 103.2 |
Meldola's blue |
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GT0259-25G | Glentham Life Sciences | 25 g | EUR 160.8 |
Meldola's blue |
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GT0259-50G | Glentham Life Sciences | 50 g | EUR 255.6 |
Bromochlorophenol Blue |
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GT2674-100MG | Glentham Life Sciences | 100 mg | EUR 93.6 |
Bromochlorophenol Blue |
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GT2674-1G | Glentham Life Sciences | 1 g | EUR 208.8 |
Bromophenol Blue |
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GT2874-100G | Glentham Life Sciences | 100 g | EUR 151.2 |
Bromophenol Blue |
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GT2874-10G | Glentham Life Sciences | 10 g | EUR 62.4 |
Bromophenol Blue |
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GT2874-25G | Glentham Life Sciences | 25 g | EUR 79.2 |
Bromophenol Blue |
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GT2874-50G | Glentham Life Sciences | 50 g | EUR 103.2 |
Bromophenol Blue |
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GT2874-5G | Glentham Life Sciences | 5 g | EUR 54 |
Hydroxynaphthol blue |
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GT3182-100G | Glentham Life Sciences | 100 g | EUR 284.4 |
Hydroxynaphthol blue |
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GT3182-10G | Glentham Life Sciences | 10 g | EUR 93.6 |
Hydroxynaphthol blue |
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GT3182-25G | Glentham Life Sciences | 25 g | EUR 132 |
Hydroxynaphthol blue |
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GT3182-50G | Glentham Life Sciences | 50 g | EUR 199.2 |
Bromothymol Blue |
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GT6837-10G | Glentham Life Sciences | 10 g | EUR 56.4 |
Bromothymol Blue |
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GT6837-25G | Glentham Life Sciences | 25 g | EUR 73.2 |
Bromothymol Blue |
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GT6837-50G | Glentham Life Sciences | 50 g | EUR 103.2 |
Bromothymol Blue |
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GT6837-5G | Glentham Life Sciences | 5 g | EUR 50.4 |
Xylenol Blue |
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GT7988-1G | Glentham Life Sciences | 1 g | EUR 55.2 |
Xylenol Blue |
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GT7988-25G | Glentham Life Sciences | 25 g | EUR 180 |
Xylenol Blue |
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GT7988-5G | Glentham Life Sciences | 5 g | EUR 93.6 |
Bromoxylenol Blue |
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GT8506-10G | Glentham Life Sciences | 10 g | EUR 170.4 |
Bromoxylenol Blue |
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GT8506-1G | Glentham Life Sciences | 1 g | EUR 55.2 |
Bromoxylenol Blue |
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GT8506-5G | Glentham Life Sciences | 5 g | EUR 112.8 |
Thymol Blue |
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GT9755-100G | Glentham Life Sciences | 100 g | EUR 180 |
Thymol Blue |
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GT9755-10G | Glentham Life Sciences | 10 g | EUR 60 |
Thymol Blue |
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GT9755-25G | Glentham Life Sciences | 25 g | EUR 84 |
Thymol Blue |
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GT9755-50G | Glentham Life Sciences | 50 g | EUR 112.8 |
Thymol Blue |
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GT9755-5G | Glentham Life Sciences | 5 g | EUR 50.4 |
Trypan blue |
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TT1140 | Bio Basic | 10g | EUR 78.79 |
10-240kDa Wide Range 3 Color Prestained Protein Ladder |
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BG00364 | Bio Basic | 500ul, 500ul | EUR 237.48 |
20 bp TinyOrange DNA Ladder (105ng/µl) |
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309-005 | GeneOn | 500 µl | EUR 129.6 |
20 bp TinyOrange DNA Ladder (105ng/µl) |
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309-025 | GeneOn | 5x500µl | EUR 387.6 |
Ready-to-Use 1 KB DNA Ladder |
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31022 | Biotium | 1kit | EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1kit |
Ready-to-Use 100 BP DNA Ladder |
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31032 | Biotium | 1kit | EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1kit |
1 kb DNA Ladder in TE buffer |
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31039 | Biotium | 300uL | EUR 169.2 |
Description: Minimum order quantity: 1 unit of 300uL |
100 bp DNA Ladder in TE buffer |
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31040 | Biotium | 300uL | EUR 169.2 |
Description: Minimum order quantity: 1 unit of 300uL |
1000bp/1kb DNA ladder (no loading dye) |
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305-005 | GeneOn | 50µg/250µl | EUR 60 |
1000bp/1kb DNA ladder (no loading dye) |
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305-025 | GeneOn | 5x50µg | EUR 144 |
1Kb Plus DNA Ladder, Ready-to-use |
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9K-004-0001 | Bio Basic | 0.5ml | EUR 193.98 |
Azura PureView 250bp DNA Ladder - 100 Lanes |
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AZ-1121 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 250bp DNA Ladder - 500 Lanes |
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AZ-1125 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 250bp DNA Ladder - 1000 Lanes |
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AZ-1125-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 100bp DNA Ladder - 100 Lanes |
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AZ-1131 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 100bp DNA Ladder - 500 Lanes |
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AZ-1135 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 100bp DNA Ladder - 1000 Lanes |
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AZ-1135-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 50bp DNA Ladder - 100 Lanes |
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AZ-1151 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 50bp DNA Ladder - 500 Lanes |
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AZ-1155 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 50bp DNA Ladder - 1000 Lanes |
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AZ-1155-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
100 BP DNA LADDER, 500UL PER KIT |
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M-DNA-100BP | CORNING | 1/pk | EUR 87.6 |
Description: Bioscience Mol Bio; DNA Ladder |
1 KB DNA LADDER, 500UL PER KIT |
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M-DNA-1KB | CORNING | 1/pk | EUR 84 |
Description: Bioscience Mol Bio; DNA Ladder |
100 bp DNA Ladder 500ul per k |
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AXY1014 | Scientific Laboratory Supplies | EACH | EUR 145.92 |
1 kb DNA Ladder 500ul per kit |
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AXY1016 | Scientific Laboratory Supplies | EACH | EUR 136.8 |
ReadiUse™ 1 Kb Plus DNA Ladder |
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60050 | AAT Bioquest | 100 ul | EUR 99 |
ReadiUse™ 1 Kb Plus DNA Ladder |
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60051 | AAT Bioquest | 2X250 ul | EUR 203 |
Container 125ml PP Blue+Label+Blue Cap |
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CON1013 | Scientific Laboratory Supplies | PK380 | EUR 208.64 |
Snap Cap 11mm Blue Si/ PTFE Blue |
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CHR3550 | Scientific Laboratory Supplies | PK1000 | EUR 1045.38 |
Snap Cap 11mm Blue Si/PTFE Blue |
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CHR3552 | Scientific Laboratory Supplies | PK1000 | EUR 436.62 |
Blue Fluorescent Protein (BFP) Antibody |
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abx412394-50ug | Abbexa | 50 ug | EUR 594 |
Blue Fluorescent protein (His tag) |
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80R-2547 | Fitzgerald | 100 ug | EUR 603.6 |
Description: Purified recombinant Human Blue Fluorescent protein |
Protein Loading Buffer Blue 2X |
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NAT1250 | Scientific Laboratory Supplies | 10X1ML | EUR 52.33 |
Embedding Cassettes |
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M480-6-BLUE | ScyTek Laboratories | 1 ea. | EUR 232.8 |
Cassettes in QuickLoad Sleeves |
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M480-6SL-BLUE | ScyTek Laboratories | 1 ea. | EUR 340.8 |
Biometric pre-stained protein ladder (5-245 kDa) for Western |
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BPL11-500 | Alpha Diagnostics | 500 ul | EUR 196.8 |
GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD |
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310010 | GeneOn | 1x500µl | EUR 159.6 |
GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD |
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310011 | GeneOn | 5x500µl | EUR 613.2 |
Container 40ml PP Blue+Label+Blue Cap Select |
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CON1025 | Scientific Laboratory Supplies | PK1000 | EUR 220.43 |
DNASE & TOLUIDINE BLUE |
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D04-103-10kg | Alphabiosciences | 10 kg | EUR 11338.8 |
DNASE & TOLUIDINE BLUE |
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D04-103-2Kg | Alphabiosciences | 2 Kg | EUR 2511.6 |
DNASE & TOLUIDINE BLUE |
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D04-103-500g | Alphabiosciences | 500 g | EUR 726 |
Blue Gravity Column |
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BSP089 | Bio Basic | 1Column, 1 UNIT | EUR 77.75 |
Brilliant Blue G |
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C5579-100000 | ApexBio | 100 g | EUR 162 |
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility. |
Thiolite™ Blue |
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21507 | AAT Bioquest | 5 mg | EUR 158.4 |
Amplite™ Blue |
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11005 | AAT Bioquest | 25 mg | EUR 211.2 |
CytoTell™ Blue |
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22251 | AAT Bioquest | 500 Tests | EUR 158.4 |
CytoTell™ Blue |
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22252 | AAT Bioquest | 1000 Tests | EUR 211.2 |
LysoBrite™ Blue |
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22642 | AAT Bioquest | 500 Tests | EUR 211.2 |
Evans Blue Tetrasodium |
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2586-1G | Biovision | EUR 151.2 |
Trypan Blue (0.4%) |
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1209-10 | Biovision | EUR 157.2 |
Dextran blue 2000 |
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20-abx082076 | Abbexa |
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Alcian Blue 8GX |
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abx082097-1g | Abbexa | 1 g | EUR 260.4 |
Alcian Blue 8GX |
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abx082417-1g | Abbexa | 1 g | EUR 243.6 |
Aniline Blue Solution |
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ABP999 | ScyTek Laboratories | 1000 ml | EUR 159.6 |
Alkali blue 6B |
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AB0016 | Bio Basic | 25g | EUR 80.88 |
Aniline Blue Solution |
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ABP125 | ScyTek Laboratories | 125 ml | EUR 86.4 |
Aniline Blue Solution |
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ABP500 | ScyTek Laboratories | 500 ml | EUR 127.2 |
C.I.Var Blue 6 |
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abx180699-25g | Abbexa | 25 g | EUR 661.2 |
C.I.Reactive Blue 19 |
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20-abx181246 | Abbexa |
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C.I.Solvent Blue 36 |
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20-abx181247 | Abbexa |
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Pigment Blue 29 |
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abx185539-100g | Abbexa | 100 g | EUR 444 |
C.I.Food Blue 1 |
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20-abx186397 | Abbexa |
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×
Fourty-eight h and 72 h of p27(KIP1) expression confirmed a attribute DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak could induce cell cycle arrest in G(1) part via upregulating expression of p27(KIP1) and subsequently result in apoptosis in HCC-9204 cells. The p27(KIP1) -GFP fusion protein may be transiently expre-ssed in HCC-9204 cells. The inducible p27(KIP1)-expressing cell line gives a mannequin to evaluate p27(KIP1) operate.
Tags: 3x flag tag 6x his tag agarose gel loading dye bam hi restriction site bl21 (de3) blasticidin invitrogen bsmbi cas9 vector cmv promoter sequence crispr pam sequence crispr vector dh5 alpha cells dsred sequence ecorv egfp clontech egfp nucleotide sequence flag tag vector g418 antibiotic gel red dna stain gibson assembly gibson kit hek293t media midiprep protocol mini prep kit miniprep dna nhe1 neb pbabe vector pbr322 plasmid map pbs sk pcdna3 0 pcdna3 2 pcr blunt ii topo pet plasmid pet101 pet28a+ pgem t easy vector pgex 4t2 pgex4t3 pgipz vector pjet vector plko.1 puro pmd2 g promoter sequence ptriex ptripz map strep ii tag t7 tag ta cloning vector tdtomato sequence virapower zsgreen