all blue protein ladder, Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.

Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.

The biopolymer chitosan is at present in widespread use due to its nontoxicity, biocompatibility and biodegradability. Subsequently, on this examine, chitosan extracted from shrimp shells was used to synthesise biogenic silver nanoparticles (AgNPs). UV-visible spectrophotometry of lowered silver nanoparticles within the colloidal answer confirmed a single peak at 400 nm, confirming the formation of AgNPs.
The presence of biomolecules liable for lowering and capping the biogenic AgNPs was confirmed by FTIR. Floor morphology of the biosynthesised AgNPs was characterised utilizing SEM, and TEM evaluation confirmed the formation of spherical shapes 17-50 nm. The presence of elemental silver within the synthesised biogenic AgNPs was confirmed utilizing EDX and the crystalline construction characterised by XRD.
Cytotoxicity of biogenic AgNPs was decided utilizing MTT and the trypan blue exclusion assay. Morphological adjustments in HepG2 cells had been detected by evaluation of the DNA ladder sample by way of gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase three and 9 protein expression outcomes confirmed cell demise by way of apoptosis. In conclusion, chitosan has the flexibility to synthesise AgNPs with in vitro apoptotic actions.

Myricetin enhances on apoptosis induced by serum deprivation in PC12 cells mediated by mitochondrial signaling pathway.

Polyphenols have many useful results and an efficient illness therapeutic auxiliary drug. Beforehand, myricetin, a polyphenol, had been reported to own numerous organic results on human physiology. Nevertheless, mechanism of myricetin on apoptosis induced in PC12 cells remains to be unclear. PC12 cells had been handled with myricetin in two focus ranges comprising 0.1 and 1 μM beneath serum-free situation. Because of this, morphological adjustments had been noticed utilizing trypan blue assay.
DNA fragmentation was decided by DNA ladder assay to judge DNA harm ranges. Western blotting outcomes confirmed that cytosolic cytochrome c which was launched from mitochondria. Subsequently, tumor suppressor gene p53, pro-apoptotic and anti-apoptotic Bcl-2 household proteins Bax and Bcl-2 had been expressed. The caspase cascade response was induced via caspase three and 9 expression. From these outcomes, it’s advised that myricetin considerably enhanced the apoptosis induced by serum deprivation in a dose-dependent method in PC12 cells.

Mechanisms of chromium hexavalent-induced apoptosis in rat testes.

Hexavalent chromium (CrVI)-containing compounds, current in industrial settings and within the setting, are often known as carcinogens and mutagens. The current examine is designed to check the speculation that oxidative stress mediates CrVI-induced apoptosis in testis. Male Wistar rats obtained an intraperitoneal injection of potassium dichromate at doses of 1 and a pair of mg kg-1.
Superoxide anion manufacturing was assessed by the willpower of the discount of cytochrome c and iodonitrotetrazolium, lipid peroxidation (LPO), metallothioneins (MTs), and catalase (CAT) exercise. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis was detected by toluidine blue staining.
The expression of Bax and Bcl-2 proteins (Pts) was additionally investigated. After 15 days of therapy, a rise of LPO and MT ranges occurred, whereas CAT exercise was decreased. Testicular tissues of handled rats confirmed pronounced degradation of the DNA into oligonucleotides as seen within the typical electrophoretic DNA ladder sample. Intense apoptosis was noticed in germinal cells of Cr-exposed rats.
Bax Pt expression was induced in spermatogonia and spermatocytes cells of CrVI-treated rats. In distinction, Bcl-2 Pt was sometimes noticed in germ cells of CrVI-exposed rats. These outcomes clearly recommend that CrVI subacute therapy causes oxidative stress in rat testis resulting in apoptosis.

Upregulation of the MCL-1S protein variant following dihydroartemisinin therapy induces apoptosis in cholangiocarcinoma cells.

The intention of the current examine was to find out whether or not dihydroartemisinin (DHA) induces apoptosis within the human cholangiocarcinoma QBC939 cell line via the regulation of myeloid cell leukemia-1 (MCL-1) expression. The inhibitory charges of DHA on QBC939 cell proliferation and the consequences of DHA on the cell demise charges at numerous DHA concentrations and following numerous therapy instances had been examined.
The speed of apoptosis and cell cycle adjustments following DHA therapy had been examined and the adjustments within the expression of MCL-1 mRNAs and MCL-1 proteins following DHA therapy had been additionally examined. The MTT assay and trypan blue staining demonstrated that DHA considerably inhibited the proliferation (P<0.05) and promoted the demise of QBC939 cells (P<0.05).
The DNA ladder assay and circulate cytometry (FCM) evaluation demonstrated that the speed of apoptosis within the experimental group was considerably elevated following DHA therapy (P<0.01). FCM evaluation additionally demonstrated that DHA therapy led to a discount within the share of QBC939 cells within the G0/G1 and G2/M phases, and the vast majority of the DNA-treated cells had been arrested within the S part of the cell cycle (P<0.01).
Western blot evaluation demonstrated that DHA therapy considerably upregulated the expression of the pro-apoptotic MCL-1S protein. In distinction, no vital distinction within the expression of the anti-apoptotic MCL-1L protein was noticed following DHA therapy. DHA affected the expression of the apoptosis-associated protein MCL-1 via a number of mechanisms. DHA therapy elevated the ratio of MCL-1S/MCL-1L protein, thus inducing apoptosis in cholangiocarcinoma cells.
all blue protein ladder, Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.

Overexpression of p27(KIP1) induced cell cycle arrest in G(1) part and subsequent apoptosis in HCC-9204 cell line.

AIM:We’ve beforehand reported that inducible over-expression of Bak could extend cell cycle in G(1) part and result in apoptosis in HCC-9204 cells. This examine is to analyze whether or not p27(KIP1) performs an essential function on this course of.METHODS:As a way to elucidate the precise operate of p27(KIP1) on this course of, a zinc inducible p27(KIP1) secure transfectant and transient p27(KIP1)-GFP fusion transfectant had been constructed.
The results of inducible p27(KIP1) on cell development, cell cycle arrest and apoptosis had been examined within the mock, management pMD vector, and pMD-KIP1 transfected HCC-9204 cells.RESULTS:This p27(KIP1)-GFP transfectant could transiently categorical the fusion gene. The cell development was lowered by 35% at 48 h of p27(KIP1) induction with zinc therapy as decided by trypan blue exclusion assay.
These variations remained the identical after 72h of p27(KIP1) expression. p27(KIP1) precipitated cell cycle arrest after 24 h of induction, with 40% improve in G(1) inhabitants. Extended p27(KIP1) expression on this cell line induced apoptotic cell demise mirrored by TUNEL assay.

1000bp/1kb DNA ladder (blue, ready-to-use)

305-105 50µg/500µl
EUR 50

1000bp/1kb DNA ladder (blue, ready-to-use)

305-105L 10x50 µg Ask for price

1000bp/1kb DNA ladder (blue, ready-to-use)

305-105XL 20x50 µg Ask for price

1000bp/1kb DNA ladder (blue, ready-to-use)

305-125 5x50 µg
EUR 120

Leukemia - ALL Exosome

P141-ALL NULL
EUR 0

10-250kDa Wide Range Blue-Red Two Color Protein Ladder, Prestained

BZ0010R 500ul, 500ul
EUR 167.45
  • Product category: Electrophoresis Related/Ladders - Protein/10-250 kDa

Leukemia - ALL Exosome RNA

P241-ALL NULL
EUR 0

10-250kDa Wide Range Blue-Red-Green Three Color Protein Ladder, Prestained

BZ0011G 500ul, 500ul
EUR 197.9
  • Product category: Electrophoresis Related/Ladders - Protein/10-250 kDa

100bp DNA ladder

9K-004-0002 0.5ml
EUR 217.48
  • Product category: Electrophoresis Related/Ladders - DNA/100-1000 bp

BriteRuler? Pre-stained Protein Ladder

9306-100
EUR 262

ECOS Blue (XL1-Blue

FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-80VL 100 µl x 10 vials Ask for price

WSE-7020 EzProtein Ladder

2332346 1unit
EUR 283
Description: Pr ot ei n col or ed M ar k er

1 kb DNA Ladder

M1157-500
EUR 278

100 bp DNA Ladder

M1158-500
EUR 278

100 bp DNA Ladder

MD104-01 250 μl
EUR 110

100 bp DNA Ladder

MD104-02 500 μl
EUR 116

iVDye 50bp DNA Ladder

V1001-001 1ml
EUR 182

iVDye 50bp DNA Ladder

V1001-025 250ul
EUR 88

iVDye 50bp DNA Ladder

V1001-100 4x250ul
EUR 192

iVDye 50bp DNA Ladder

V1001-250 10X250ul
EUR 352

iVDye 100bp DNA Ladder

V1002-001 1ml
EUR 166

iVDye 100bp DNA Ladder

V1002-025 250ul
EUR 80

iVDye 100bp DNA Ladder

V1002-100 4x250ul
EUR 163

iVDye 100bp DNA Ladder

V1002-250 10X250ul
EUR 315

iVDye 1Kb DNA Ladder

V1003-001 1ml
EUR 124

iVDye 1Kb DNA Ladder

V1003-025 250ul
EUR 76

iVDye 1Kb DNA Ladder

V1003-100 4x250ul
EUR 131

iVDye 1Kb DNA Ladder

V1003-250 10x250ul
EUR 228

iVDye 1Kb DNA Ladder

V1003-500 20x250ul
EUR 381

1 kb DNA Ladder

Z6030002 100 lanes
EUR 217

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests 25 tests
EUR 244
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Blue Fluorescent Protein (BFP)

4994-100
EUR 376

Blue Fluorescent Protein (BFP)

4994-1000
EUR 2393

Blue Fluorescent Protein (BFP)

4994-5000
EUR 6961

BriteRuler? 1 kb DNA Ladder

9301-100
EUR 262

Star 1kb DNA Ladder Express

BT10701 500µl
EUR 80
Description: High purity buffer for various PCR applications.

1 Kb Ladder DNA Marker

FYD001-500UL 20 µg/ 500 µl Ask for price

100 bp Ladder DNA Marker

FYD002-500UL 30 µg/ 500 µl Ask for price

50 bp Ladder DNA Marker

FYD004-500UL 32.5 µg/ 500 µl Ask for price

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 502

Apoptotic DNA Ladder Isolation Kit

K170-50
EUR 468

Phthalocyanine blue

20-abx181340
  • EUR 328.00
  • EUR 217.00
  • EUR 746.00
  • 100 g
  • 25 g
  • 500 g
  • Shipped within 1-2 weeks.

Evans Blue

abx186080-125g 125g
EUR 453
  • Shipped within 1-2 weeks.

Bromophenol blue

abx183916-500ml 500 ml
EUR 230
  • Shipped within 1-2 weeks.

Bromophenol blue

abx183916-5l 5l
EUR 592
  • Shipped within 1-2 weeks.

Evans Blue

20-abx082082
  • EUR 175.00
  • EUR 217.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Bromphenol Blue

20-abx082184
  • EUR 230.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Evans Blue

20-abx082237
  • EUR 189.00
  • EUR 230.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Bromphenol Blue

20-abx082376
  • EUR 244.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Toluidine Blue

abx082425-5g 5 g
EUR 189
  • Shipped within 5-10 working days.

Trypan Blue

20-abx082476
  • EUR 230.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Bromophenol Blue

abx082607-10g 10 g
EUR 203
  • Shipped within 5-10 working days.

Bromophenol Blue

CH019 25 g
EUR 132

Bromophenol Blue

CH020 50 g
EUR 166

Bromophenol Blue

CH021 100 g
EUR 227

Bromophenol Blue

B7774-5000 5 g
EUR 87
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye.

Methylene Blue

HY-14536 100mg
EUR 119

Thiazolyl Blue

HY-15924 10mM/1mL
EUR 113

Evans Blue

HY-B1102 100mg
EUR 119

Bromothymol Blue

HY-D0012 1g
EUR 119

Diphenyl Blue

HY-D0970 10mM/1mL
EUR 113

Calcein Blue

HY-101887 25mg
EUR 119

Bromochlorophenol Blue

GT2674-100MG 100 mg
EUR 78

Bromochlorophenol Blue

GT2674-1G 1 g
EUR 174

Bromophenol Blue

GT2874-100G 100 g
EUR 126

Bromophenol Blue

GT2874-10G 10 g
EUR 52

Bromophenol Blue

GT2874-25G 25 g
EUR 66

Bromophenol Blue

GT2874-50G 50 g
EUR 86

Bromophenol Blue

GT2874-5G 5 g
EUR 45

Hydroxynaphthol blue

GT3182-100G 100 g
EUR 237

Hydroxynaphthol blue

GT3182-10G 10 g
EUR 78

Hydroxynaphthol blue

GT3182-25G 25 g
EUR 110

Hydroxynaphthol blue

GT3182-50G 50 g
EUR 166

Bromothymol Blue

GT6837-10G 10 g
EUR 47

Bromothymol Blue

GT6837-25G 25 g
EUR 61

Bromothymol Blue

GT6837-50G 50 g
EUR 86

Bromothymol Blue

GT6837-5G 5 g
EUR 42

Xylenol Blue

GT7988-1G 1 g
EUR 46

Xylenol Blue

GT7988-25G 25 g
EUR 150

Xylenol Blue

GT7988-5G 5 g
EUR 78

Bromoxylenol Blue

GT8506-10G 10 g
EUR 142

Bromoxylenol Blue

GT8506-1G 1 g
EUR 46

Bromoxylenol Blue

GT8506-5G 5 g
EUR 94

Thymol Blue

GT9755-100G 100 g
EUR 150

Thymol Blue

GT9755-10G 10 g
EUR 50

Thymol Blue

GT9755-25G 25 g
EUR 70

Thymol Blue

GT9755-50G 50 g
EUR 94

Thymol Blue

GT9755-5G 5 g
EUR 42

Bromophenol Blue

G114 5.0 g
EUR 102

Meldola's blue

GT0259-10G 10 g
EUR 86

Meldola's blue

GT0259-25G 25 g
EUR 134
Fourty-eight h and 72 h of p27(KIP1) expression confirmed a attribute DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak could induce cell cycle arrest in G(1) part via upregulating expression of p27(KIP1) and subsequently result in apoptosis in HCC-9204 cells. The p27(KIP1) -GFP fusion protein may be transiently expre-ssed in HCC-9204 cells. The inducible p27(KIP1)-expressing cell line gives a mannequin to evaluate p27(KIP1) operate.

Leave a Reply

Your email address will not be published. Required fields are marked *