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Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells.
Lieven
- 0
The biopolymer chitosan is at present in widespread use due to its nontoxicity, biocompatibility and biodegradability. Subsequently, on this examine, chitosan extracted from shrimp shells was used to synthesise biogenic silver nanoparticles (AgNPs). UV-visible spectrophotometry of lowered silver nanoparticles within the colloidal answer confirmed a single peak at 400 nm, confirming the formation of AgNPs.
The presence of biomolecules liable for lowering and capping the biogenic AgNPs was confirmed by FTIR. Floor morphology of the biosynthesised AgNPs was characterised utilizing SEM, and TEM evaluation confirmed the formation of spherical shapes 17-50 nm. The presence of elemental silver within the synthesised biogenic AgNPs was confirmed utilizing EDX and the crystalline construction characterised by XRD.
Cytotoxicity of biogenic AgNPs was decided utilizing MTT and the trypan blue exclusion assay. Morphological adjustments in HepG2 cells had been detected by evaluation of the DNA ladder sample by way of gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase three and 9 protein expression outcomes confirmed cell demise by way of apoptosis. In conclusion, chitosan has the flexibility to synthesise AgNPs with in vitro apoptotic actions.
Myricetin enhances on apoptosis induced by serum deprivation in PC12 cells mediated by mitochondrial signaling pathway.
Polyphenols have many useful results and an efficient illness therapeutic auxiliary drug. Beforehand, myricetin, a polyphenol, had been reported to own numerous organic results on human physiology. Nevertheless, mechanism of myricetin on apoptosis induced in PC12 cells remains to be unclear. PC12 cells had been handled with myricetin in two focus ranges comprising 0.1 and 1 μM beneath serum-free situation. Because of this, morphological adjustments had been noticed utilizing trypan blue assay.
DNA fragmentation was decided by DNA ladder assay to judge DNA harm ranges. Western blotting outcomes confirmed that cytosolic cytochrome c which was launched from mitochondria. Subsequently, tumor suppressor gene p53, pro-apoptotic and anti-apoptotic Bcl-2 household proteins Bax and Bcl-2 had been expressed. The caspase cascade response was induced via caspase three and 9 expression. From these outcomes, it’s advised that myricetin considerably enhanced the apoptosis induced by serum deprivation in a dose-dependent method in PC12 cells.
Mechanisms of chromium hexavalent-induced apoptosis in rat testes.
Hexavalent chromium (CrVI)-containing compounds, current in industrial settings and within the setting, are often known as carcinogens and mutagens. The current examine is designed to check the speculation that oxidative stress mediates CrVI-induced apoptosis in testis. Male Wistar rats obtained an intraperitoneal injection of potassium dichromate at doses of 1 and a pair of mg kg-1.
Superoxide anion manufacturing was assessed by the willpower of the discount of cytochrome c and iodonitrotetrazolium, lipid peroxidation (LPO), metallothioneins (MTs), and catalase (CAT) exercise. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis was detected by toluidine blue staining.
The expression of Bax and Bcl-2 proteins (Pts) was additionally investigated. After 15 days of therapy, a rise of LPO and MT ranges occurred, whereas CAT exercise was decreased. Testicular tissues of handled rats confirmed pronounced degradation of the DNA into oligonucleotides as seen within the typical electrophoretic DNA ladder sample. Intense apoptosis was noticed in germinal cells of Cr-exposed rats.
Bax Pt expression was induced in spermatogonia and spermatocytes cells of CrVI-treated rats. In distinction, Bcl-2 Pt was sometimes noticed in germ cells of CrVI-exposed rats. These outcomes clearly recommend that CrVI subacute therapy causes oxidative stress in rat testis resulting in apoptosis.
Upregulation of the MCL-1S protein variant following dihydroartemisinin therapy induces apoptosis in cholangiocarcinoma cells.
The intention of the current examine was to find out whether or not dihydroartemisinin (DHA) induces apoptosis within the human cholangiocarcinoma QBC939 cell line via the regulation of myeloid cell leukemia-1 (MCL-1) expression. The inhibitory charges of DHA on QBC939 cell proliferation and the consequences of DHA on the cell demise charges at numerous DHA concentrations and following numerous therapy instances had been examined.
The speed of apoptosis and cell cycle adjustments following DHA therapy had been examined and the adjustments within the expression of MCL-1 mRNAs and MCL-1 proteins following DHA therapy had been additionally examined. The MTT assay and trypan blue staining demonstrated that DHA considerably inhibited the proliferation (P<0.05) and promoted the demise of QBC939 cells (P<0.05).
The DNA ladder assay and circulate cytometry (FCM) evaluation demonstrated that the speed of apoptosis within the experimental group was considerably elevated following DHA therapy (P<0.01). FCM evaluation additionally demonstrated that DHA therapy led to a discount within the share of QBC939 cells within the G0/G1 and G2/M phases, and the vast majority of the DNA-treated cells had been arrested within the S part of the cell cycle (P<0.01).
Western blot evaluation demonstrated that DHA therapy considerably upregulated the expression of the pro-apoptotic MCL-1S protein. In distinction, no vital distinction within the expression of the anti-apoptotic MCL-1L protein was noticed following DHA therapy. DHA affected the expression of the apoptosis-associated protein MCL-1 via a number of mechanisms. DHA therapy elevated the ratio of MCL-1S/MCL-1L protein, thus inducing apoptosis in cholangiocarcinoma cells.

Overexpression of p27(KIP1) induced cell cycle arrest in G(1) part and subsequent apoptosis in HCC-9204 cell line.
AIM:We’ve beforehand reported that inducible over-expression of Bak could extend cell cycle in G(1) part and result in apoptosis in HCC-9204 cells. This examine is to analyze whether or not p27(KIP1) performs an essential function on this course of.METHODS:As a way to elucidate the precise operate of p27(KIP1) on this course of, a zinc inducible p27(KIP1) secure transfectant and transient p27(KIP1)-GFP fusion transfectant had been constructed.
The results of inducible p27(KIP1) on cell development, cell cycle arrest and apoptosis had been examined within the mock, management pMD vector, and pMD-KIP1 transfected HCC-9204 cells.RESULTS:This p27(KIP1)-GFP transfectant could transiently categorical the fusion gene. The cell development was lowered by 35% at 48 h of p27(KIP1) induction with zinc therapy as decided by trypan blue exclusion assay.
These variations remained the identical after 72h of p27(KIP1) expression. p27(KIP1) precipitated cell cycle arrest after 24 h of induction, with 40% improve in G(1) inhabitants. Extended p27(KIP1) expression on this cell line induced apoptotic cell demise mirrored by TUNEL assay.
BriteRuler? Pre-stained Protein Ladder |
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9306-100 | Biovision | each | EUR 314.4 |
100bp DNA ladder |
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9K-004-0002 | Bio Basic | 0.5ml | EUR 260.98 |
Biometric pre-stained protein ladder (5-245 kDa) for Western |
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BPL11-500 | Alpha Diagnostics | 500 ul | EUR 196.8 |
1 kb DNA Ladder |
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M1157-500 | Biovision | each | EUR 333.6 |
1 kb DNA Ladder |
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Z6030002 | Biochain | 100 lanes | EUR 158 |
100 bp DNA Ladder |
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M1158-500 | Biovision | each | EUR 333.6 |
100 bp DNA Ladder |
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MD104-01 | Vazyme | 250 μl | EUR 132 |
100 bp DNA Ladder |
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MD104-02 | Vazyme | 500 μl | EUR 139.2 |
iVDye 1Kb DNA Ladder |
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V1003-001 | GenDepot | 1ml | EUR 148.8 |
iVDye 1Kb DNA Ladder |
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V1003-025 | GenDepot | 250ul | EUR 91.2 |
iVDye 1Kb DNA Ladder |
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V1003-100 | GenDepot | 4x250ul | EUR 157.2 |
iVDye 1Kb DNA Ladder |
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V1003-250 | GenDepot | 10x250ul | EUR 273.6 |
iVDye 1Kb DNA Ladder |
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V1003-500 | GenDepot | 20x250ul | EUR 457.2 |
iVDye 50bp DNA Ladder |
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V1001-001 | GenDepot | 1ml | EUR 218.4 |
iVDye 50bp DNA Ladder |
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V1001-025 | GenDepot | 250ul | EUR 105.6 |
iVDye 50bp DNA Ladder |
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V1001-100 | GenDepot | 4x250ul | EUR 230.4 |
iVDye 50bp DNA Ladder |
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V1001-250 | GenDepot | 10X250ul | EUR 422.4 |
iVDye 100bp DNA Ladder |
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V1002-001 | GenDepot | 1ml | EUR 199.2 |
iVDye 100bp DNA Ladder |
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V1002-025 | GenDepot | 250ul | EUR 96 |
iVDye 100bp DNA Ladder |
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V1002-100 | GenDepot | 4x250ul | EUR 195.6 |
iVDye 100bp DNA Ladder |
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V1002-250 | GenDepot | 10X250ul | EUR 378 |
10-240kDa Wide Range 3 Color Prestained Protein Ladder |
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BG00364 | Bio Basic | 500ul, 500ul | EUR 237.48 |
WSE-7020 EzProtein Ladder |
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2332346 | Atto | 1unit | EUR 339.6 |
Description: Pr ot ei n col or ed M ar k er |
100 bp Ladder DNA Marker |
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FYD002-500UL | Yeastern Biotech | 30 µg/ 500 µl | Ask for price |
1 Kb Ladder DNA Marker |
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FYD001-500UL | Yeastern Biotech | 20 µg/ 500 µl | Ask for price |
50 bp Ladder DNA Marker |
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FYD004-500UL | Yeastern Biotech | 32.5 µg/ 500 µl | Ask for price |
GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD |
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310010 | GeneOn | 1x500µl | EUR 159.6 |
GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD |
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310011 | GeneOn | 5x500µl | EUR 613.2 |
Un-stained protein ladder (14-116 kDa) for mol wt determination by Western |
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PMU11-500 | Alpha Diagnostics | 50 loads | EUR 110.4 |
BriteRuler? 1 kb DNA Ladder |
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9301-100 | Biovision | each | EUR 314.4 |
Star 1kb DNA Ladder Express |
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BT10701 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
100 bp DNA Ladder 500ul per k |
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AXY1014 | Scientific Laboratory Supplies | EACH | EUR 158.4 |
1 kb DNA Ladder 500ul per kit |
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AXY1016 | Scientific Laboratory Supplies | EACH | EUR 148.8 |
1 KB DNA LADDER, 500UL PER KIT |
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M-DNA-1KB | CORNING | 1/pk | EUR 84 |
Description: Bioscience Mol Bio; DNA Ladder |
GENE`s Protein Ladder PS10 PLUS prestained, 10 bands, 11 - 180kD |
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310003 | GeneOn | 1x500 µl | EUR 144 |
GENE`s Protein Ladder PS10 PLUS prestained, 10 bands, 11 - 180kD |
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310004 | GeneOn | 5x500µl | EUR 550.8 |
GENE`s Plus Protein Ladder PS11 prestained, 11 bands, 10 - 175kD |
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310005 | GeneOn | 1x500µl | EUR 144 |
GENE`s Plus Protein Ladder PS11 prestained, 11 bands, 10 - 175kD |
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310006 | GeneOn | 5x500µl | EUR 550.8 |
100 BP DNA LADDER, 500UL PER KIT |
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M-DNA-100BP | CORNING | 1/pk | EUR 87.6 |
Description: Bioscience Mol Bio; DNA Ladder |
GENE`s Protein Ladder Wide Range, prestained, 10 bands, 6,5 - 270kD |
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310020 | GeneOn | 1x500µl | EUR 153.6 |
GENE`s Protein Ladder Wide Range, prestained, 10 bands, 6,5 - 270kD |
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310021 | GeneOn | 5x500µl | EUR 550.8 |
Wide range 1 kb DNA Ladder |
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M1191-500 | Biovision | each | EUR 333.6 |
Wide range 100 bp DNA Ladder |
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M1192-500 | Biovision | each | EUR 333.6 |
Azura PureView 250bp DNA Ladder - 1000 Lanes |
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AZ-1125-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 100bp DNA Ladder - 1000 Lanes |
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AZ-1135-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 50bp DNA Ladder - 1000 Lanes |
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AZ-1155-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
20 bp TinyOrange DNA Ladder (105ng/µl) |
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309-005 | GeneOn | 500 µl | EUR 129.6 |
20 bp TinyOrange DNA Ladder (105ng/µl) |
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309-025 | GeneOn | 5x500µl | EUR 387.6 |
Star 100 bp DNA Ladder Express |
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BA01302 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
Apoptotic DNA Ladder Isolation Kit |
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K170-50 | Biovision | each | EUR 561.6 |
Apoptotic DNA Ladder Isolation Kit |
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K2045-50 | ApexBio | 50 assays | EUR 602.4 |
Biometric un-stained protein ladder (10-200 kDa) for mol wt determination by Western |
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PMU12-50 | Alpha Diagnostics | 50 loads | EUR 110.4 |
50 kb large-Range DNA ladder |
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308-025 | GeneOn | 250 µg | EUR 144 |
Azura PureView 250bp DNA Ladder - 100 Lanes |
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AZ-1121 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 250bp DNA Ladder - 500 Lanes |
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AZ-1125 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 100bp DNA Ladder - 100 Lanes |
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AZ-1131 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 100bp DNA Ladder - 500 Lanes |
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AZ-1135 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 50bp DNA Ladder - 100 Lanes |
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AZ-1151 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 50bp DNA Ladder - 500 Lanes |
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AZ-1155 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 1 Kb DNA Ladder - 1000 Lanes |
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AZ-1105-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
1 kb DNA Ladder in TE buffer |
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31039 | Biotium | 300uL | EUR 169.2 |
Description: Minimum order quantity: 1 unit of 300uL |
Azura PureView 1 Kb DNA Ladder - 100 Lanes |
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AZ-1101 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 1 Kb DNA Ladder - 500 Lanes |
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AZ-1105 | Real Time Primers | 500 Lanes | EUR 277.2 |
1000bp/1kb DNA ladder (no loading dye) |
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305-005 | GeneOn | 50µg/250µl | EUR 60 |
1000bp/1kb DNA ladder (no loading dye) |
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305-025 | GeneOn | 5x50µg | EUR 144 |
Quick Apoptotic DNA Ladder Detection Kit |
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K120-50 | Biovision | each | EUR 548.4 |
Quick Apoptotic DNA Ladder Detection Kit |
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K2194-50 | ApexBio | 50 assays | EUR 568.8 |
1Kb Plus DNA Ladder, Ready-to-use |
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9K-004-0001 | Bio Basic | 0.5ml | EUR 193.98 |
Enhanced Apoptotic DNA Ladder Detection Kit |
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K130-50 | Biovision | each | EUR 529.2 |
Enhanced Apoptotic DNA Ladder Detection Kit |
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K2202-50 | ApexBio | 50 assays | EUR 568.8 |
100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye) |
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W0636-100 | 101Bio | - | Ask for price |
Multicolor, prestained, His-tagged Protein ladder (20 Kda-140 kda) for Western blot |
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HISM20-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Accuris SmartCheck™ 1kb DNA Ladder, 500ul / 100 lanes |
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PR4100-100 | Benchmark Scientific | 1 PC | EUR 40.3 |
Accuris SmartCheck™ 50bp DNA Ladder, 500ul / 100 lanes |
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PR4005-100 | Benchmark Scientific | 1 PC | EUR 40.3 |
Accuris SmartCheck™ 100bp DNA Ladder, 500ul / 100 lanes |
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PR4010-100 | Benchmark Scientific | 1 PC | EUR 40.3 |
50bp DNA ladder (no loading dye) (50bp - 1000 bp) |
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300009 | GeneOn | 50µg/250µl | EUR 60 |
50bp DNA ladder (no loading dye) (50bp - 1000 bp) |
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300010 | GeneOn | 5x50 µg | EUR 144 |
100bp plus Ladder (no loading dye, 100-1500 bp) |
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306-005 | GeneOn | 50µg/250µl | EUR 60 |
100bp plus Ladder (no loading dye, 100-1500 bp) |
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306-025 | GeneOn | 5x50 µg | EUR 144 |
50/100 bp DNA Ladder (50 bp ~ 1,500 bp, with loading dye) |
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W109-100 | 101Bio | - | Ask for price |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
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300005 | GeneOn | 86µg/500µl | EUR 84 |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
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300006 | GeneOn | 5x86 µg | EUR 253.2 |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
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300006L | GeneOn | 10x86 µg | EUR 465.6 |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
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300006XL | GeneOn | 20x86 µg | EUR 894 |
Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500ul / 500 lanes |
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PR4100-500 | Benchmark Scientific | 1 PC | EUR 180.4 |
Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500ul / 500 lanes |
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PR4005-500 | Benchmark Scientific | 1 PC | EUR 180.4 |
Accuris SmartCheck™ 100bp DNA Ladder, 5 x 500ul / 500 lanes |
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PR4010-500 | Benchmark Scientific | 1 PC | EUR 180.4 |
OneMark 100 DNA Ladder (ready-to-use, 100-3000 bp) |
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DM101-0100 | GeneOn | 600 µl | EUR 75.6 |
OneMark B DNA Ladder (ready-to-use, 250-1000 kb) |
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DM110-0100 | GeneOn | 600 µl | EUR 75.6 |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
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307-105 | GeneOn | 50µg/500µl | EUR 60 |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
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307-105L | GeneOn | 10x50 µg | Ask for price |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
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307-105XL | GeneOn | 20x50 µg | Ask for price |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
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307-125 | GeneOn | 5x50µg | EUR 144 |
XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes) |
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300007 | GeneOn | 50µg/500µl | EUR 84 |
XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes) |
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300008 | GeneOn | 5x50 µg | EUR 253.2 |
ACTGene? DNA Marker, 100 bp Ladder, 11 Fragments, 100 loads |
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ACT-IDMWD100 | ACTGene | each | EUR 178.8 |
1 kb DNA Ladder 2.0 (0.5 kb ~ 10 kb, with loading dye) |
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W108-100 | 101Bio | - | Ask for price |
1 kb DNA Ladder 2.0 (0.5 kb ~ 10 kb, with loading dye) |
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W108-500 | 101Bio | - | Ask for price |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
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300003 | GeneOn | 50µg/500µl | EUR 84 |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
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300003L | GeneOn | 10x50 µg | Ask for price |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
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300003XL | GeneOn | 20x50 µg | Ask for price |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
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300004 | GeneOn | 5x50 µg | EUR 253.2 |
ACTGene? DNA Marker, 1 kb Ladder, 13 Fragments, 100 loads |
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ACT-IDMWD1 | ACTGene | each | EUR 178.8 |
ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads |
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ACT-IDMWD2 | ACTGene | each | EUR 178.8 |
100 bp DNA Ladder Plus, ready-to-use, 50 ug, 0.5 ug per lane |
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Z6030001-1 | Biochain | 100 lanes | EUR 56 |
100 bp DNA Ladder Plus, ready-to-use, 250 ug, 0.5 ug per lane |
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Z6030001-3 | Biochain | 500 lanes | EUR 224 |
100 bp DNA Ladder Plus, loading dye included, 50 ug, 0.5 ug per lane |
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Z6030001-2 | Biochain | 100 lanes | EUR 50 |
100 bp DNA Ladder Plus, loading dye included, 250 ug, 0.5 ug per lane |
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Z6030001-4 | Biochain | 500 lanes | EUR 198 |
Blue Fluorescent Protein (BFP) |
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4994-100 | Biovision | each | EUR 451.2 |
Blue Fluorescent Protein (BFP) |
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4994-1000 | Biovision | each | EUR 2871.6 |
Blue Fluorescent Protein (BFP) |
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4994-5000 | Biovision | each | EUR 8353.2 |
Protein Loading Buffer Blue 2X |
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NAT1250 | Scientific Laboratory Supplies | 10X1ML | EUR 69.6 |
Blue Fluorescent protein (His tag) |
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80R-2547 | Fitzgerald | 100 ug | EUR 603.6 |
Description: Purified recombinant Human Blue Fluorescent protein |
One-Step Blue Protein Gel Stain, 1X |
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21003-1L | Biotium | 1L | EUR 178.8 |
Description: Minimum order quantity: 1 unit of 1L |
One-Step Blue Protein Gel Stain, 1X |
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21003-F | Biotium | 25ML | EUR 60 |
Description: Minimum order quantity: 1 unit of 25ML |
Blue Fluorescent Protein (BFP) Antibody |
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abx412394-50ug | Abbexa | 50 ug | EUR 594 |
Protect Beads Blue |
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SWA1158 | Scientific Laboratory Supplies | PK80 | EUR 121.2 |
Blue Food Ladles. Box of 40 |
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SAM1403 | Scientific Laboratory Supplies | PK40 | EUR 68.4 |
ECOS Blue (XL1-Blue |
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FYE107-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE107-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE108-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE108-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE109-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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FYE109-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
100 bp LadderDNA Marker HighConcentration |
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FYD005-500UL | Yeastern Biotech | 64 µg/ 500 µl | Ask for price |
Snap Cap 11mm Blue Si/ PTFE Blue |
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CHR3550 | Scientific Laboratory Supplies | PK1000 | EUR 1298.4 |
Snap Cap 11mm Blue Si/PTFE Blue |
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CHR3552 | Scientific Laboratory Supplies | PK1000 | EUR 561.6 |
1L ProtoStain Blue |
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NAT1112 | Scientific Laboratory Supplies | 1L | EUR 155.37 |
SmartCheck™ Blue Protein Marker, 10 bands 9-180 kDA, 2x250ul |
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PR5010-BL | Benchmark Scientific | 1 each | EUR 127.3 |
SmartCheck™ Blue Protein Marker, 11 bands 9-180 kDA, 2x250ul |
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PR5011-BL | Benchmark Scientific | 1 each | EUR 131.6 |
Container 125ml PP Blue+Label+Blue Cap |
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CON1013 | Scientific Laboratory Supplies | PK380 | EUR 241.2 |
Sterile Blue Food Ladles. Box of 40 |
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SAM1404 | Scientific Laboratory Supplies | PK40 | EUR 102 |
Protein Stain-EZG Rapid Coomassie Blue Stain Solution |
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BSP041 | Bio Basic | 1KIT, 10prep | EUR 91.32 |
Evans Blue |
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20-abx082082 | Abbexa |
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Evans Blue |
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20-abx082237 | Abbexa |
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Evans Blue |
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abx186080-125g | Abbexa | 125g | EUR 543.6 |
Evans Blue |
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HY-B1102 | MedChemExpress | 100mg | EUR 142.8 |
Dextran blue 2000 |
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20-abx082076 | Abbexa |
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Trypan Blue |
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20-abx082476 | Abbexa |
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Thymol Blue |
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GT9755-100G | Glentham Life Sciences | 100 g | EUR 180 |
Thymol Blue |
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GT9755-10G | Glentham Life Sciences | 10 g | EUR 60 |
Thymol Blue |
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GT9755-25G | Glentham Life Sciences | 25 g | EUR 84 |
Thymol Blue |
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GT9755-50G | Glentham Life Sciences | 50 g | EUR 112.8 |
Thymol Blue |
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GT9755-5G | Glentham Life Sciences | 5 g | EUR 50.4 |
Trypan blue |
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TT1140 | Bio Basic | 10g | EUR 78.79 |
Calcein Blue |
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HY-101887 | MedChemExpress | 25mg | EUR 142.8 |
Xylenol Blue |
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GT7988-1G | Glentham Life Sciences | 1 g | EUR 55.2 |
Xylenol Blue |
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GT7988-25G | Glentham Life Sciences | 25 g | EUR 180 |
Xylenol Blue |
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GT7988-5G | Glentham Life Sciences | 5 g | EUR 93.6 |
Meldola's blue |
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GT0259-10G | Glentham Life Sciences | 10 g | EUR 103.2 |
Meldola's blue |
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GT0259-25G | Glentham Life Sciences | 25 g | EUR 160.8 |
Meldola's blue |
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GT0259-50G | Glentham Life Sciences | 50 g | EUR 255.6 |
Diphenyl Blue |
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HY-D0970 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Blue/Red/Green proteins marker - 500 uL |
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DD523005 | Scientific Laboratory Supplies | EACH | EUR 206.4 |
Toluidine Blue |
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abx082425-5g | Abbexa | 5 g | EUR 226.8 |
Methylene Blue |
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HY-14536 | MedChemExpress | 100mg | EUR 142.8 |
Thiazolyl Blue |
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HY-15924 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Bromphenol Blue |
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20-abx082184 | Abbexa |
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Bromphenol Blue |
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20-abx082376 | Abbexa |
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Disperse Blue 124 |
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LCS-4848 | Scientific Laboratory Supplies | EACH | EUR 388.8 |
Disperse Blue 148 |
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HY-D0569 | MedChemExpress | 10mM/1mL | EUR 206.4 |
Bromophenol Blue |
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abx082607-10g | Abbexa | 10 g | EUR 243.6 |
Bromophenol blue |
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BB2230 | Bio Basic | 25g | EUR 82.97 |
Bromophenol Blue |
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B7774-5000 | ApexBio | 5 g | EUR 104.4 |
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye. |
Bromophenol Blue |
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CH019 | ABM | 25 g | EUR 158.4 |
Bromophenol Blue |
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CH020 | ABM | 50 g | EUR 199.2 |
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Fourty-eight h and 72 h of p27(KIP1) expression confirmed a attribute DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak could induce cell cycle arrest in G(1) part via upregulating expression of p27(KIP1) and subsequently result in apoptosis in HCC-9204 cells. The p27(KIP1) -GFP fusion protein may be transiently expre-ssed in HCC-9204 cells. The inducible p27(KIP1)-expressing cell line gives a mannequin to evaluate p27(KIP1) operate.
Tags: 3x flag tag 6x his tag agarose gel loading dye bam hi restriction site bl21 (de3) blasticidin invitrogen bsmbi cas9 vector cmv promoter sequence crispr pam sequence crispr vector dh5 alpha cells dsred sequence ecorv egfp clontech egfp nucleotide sequence flag tag vector g418 antibiotic gel red dna stain gibson assembly gibson kit hek293t media midiprep protocol mini prep kit miniprep dna nhe1 neb pbabe vector pbr322 plasmid map pbs sk pcdna3 0 pcdna3 2 pcr blunt ii topo pet plasmid pet101 pet28a+ pgem t easy vector pgex 4t2 pgex4t3 pgipz vector pjet vector plko.1 puro pmd2 g promoter sequence ptriex ptripz map strep ii tag t7 tag ta cloning vector tdtomato sequence virapower zsgreen