Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

The Wnt/β-catenin pathway is a extremely conserved, incessantly mutated developmental and most cancers pathway. Its output is outlined primarily by β-catenin’s phosphorylation- and ubiquitylation-dependent proteasomal degradation, initiated by the multi-protein β-catenin destruction advanced.
The exact mechanisms underlying destruction advanced perform have remained unknown, largely due to the shortage of appropriate in vitro techniques. Right here we describe the in vitro reconstitution of an energetic human β-catenin destruction advanced from purified parts, recapitulating advanced meeting, β-catenin modification, and degradation.
We reveal that AXIN1 polymerization and APC promote β-catenin seize, phosphorylation, and ubiquitylation. APC facilitates β-catenin’s flux by the advanced by limiting ubiquitylation processivity and straight interacts with the SCFβ-TrCP E3 ligase advanced in a β-TrCP-dependent method.
Oncogenic APC truncation variants, though a part of the advanced, are functionally impaired. Nonetheless, even essentially the most severely truncated APC variant promotes β-catenin recruitment. These findings exemplify the ability of biochemical reconstitution to interrogate the molecular mechanisms of Wnt/β-catenin signaling.

A Community Pharmacology Strategy to Examine the Mechanism of Erjing Prescription in Sort 2 Diabetes

Erjing prescription (EJP) was an historical system that was recorded within the Normal Medical Assortment of Royal Benevolence of the Track Dynasty. It has been incessantly used to deal with sort 2 diabetes mellitus (T2DM) within the lengthy historical past of China.
The system consists of Lycium barbarum L. and Polygonatum sibiricum F. Delaroche with a ratio of 1 : 1. This examine aimed to establish the potential results and mechanisms of EJP remedy T2DM. The goal proteins and potential pathways of EJP in T2DM remedy have been investigated by the strategy of community pharmacology and real-time PCR (RT-PCR).
99 diabetes-related proteins have been regulated by 56 bioactive constituents in EJP in 26 sign pathways by Cytoscape dedication. In accordance with GO evaluation, 606 genes entries have been enriched. The PPI community prompt that AKT1, EGF, EGFR, MAPK1, and GSK3β proteins have been core genes. Among the many 26 sign pathways, the PI3K-AKT sign pathway was examined by the RT-PCR.
The expression stage of PI3K p85, AKT1, GSK3β, and Myc mRNA of this pathway was regulated by EJP. The examine based mostly on community pharmacology and RT-PCR evaluation revealed that the blood sugar stage was regulated by EJP by way of regulating the PI3K-AKT sign pathway. Loads of new remedy strategies for T2DM utilizing EJP have been supplied by community pharmacology evaluation.
Acquired resistance to MAPK inhibitors limits the medical efficacy in melanoma remedy. We and others have lately proven that BRAF inhibitor (BRAFi)-resistant melanoma cells can develop a dependency on the therapeutic medication to which they’ve acquired resistance, making a vulnerability for these cells that may probably be exploited in most cancers remedy.
In drug-addicted melanoma cells, it was proven that this induction of cell loss of life was preceded by a particular ERK2-dependent phenotype change; nevertheless, the underlying molecular mechanisms are largely missing.
To extend the molecular understanding of this drug dependency, we utilized a mass spectrometry-based proteomic strategy on BRAFi-resistant BRAFMUT 451Lu cells, during which ERK1, ERK2, and JUNB have been silenced individually utilizing CRISPR-Cas9.
Inactivation of ERK2 and, to a lesser extent, JUNB prevents drug dependancy in these melanoma cells, whereas, conversely, knockout of ERK1 fails to reverse this phenotype, exhibiting a response much like that of management cells.
Our evaluation reveals that ERK2 and JUNB share comparable proteome responses dominated by reactivation of cell division. Importantly, we discover that EMT activation in drug-addicted melanoma cells upon drug withdrawal is affected by silencing ERK2 however not ERK1.
Furthermore, transcription issue (regulator) enrichment reveals that PIR acts as an effector of ERK2 and phosphoproteome evaluation reveals that silencing of ERK2 however not ERK1 results in amplification of GSK3 kinase exercise. Our outcomes depict potential mechanisms of drug dependancy in melanoma, which can present a information for therapeutic methods in drug-resistant melanoma.

Inhibition of AKT Signaling Alters βIV Spectrin Distribution on the AIS and Will increase Neuronal Excitability

The axon preliminary section (AIS) is a extremely regulated subcellular area required for neuronal firing. Adjustments within the AIS protein composition and distribution are a type of structural plasticity, which powerfully regulates neuronal exercise and will underlie a number of neuropsychiatric and neurodegenerative problems.
Regardless of its physiological and pathophysiological relevance, the signaling pathways mediating AIS protein distribution are nonetheless poorly studied. Right here, we used confocal imaging and whole-cell patch clamp electrophysiology in main hippocampal neurons to check how AIS protein composition and neuronal firing diversified in response to chose kinase inhibitors focusing on the AKT/GSK3 pathway, which has beforehand been proven to phosphorylate AIS proteins.
Picture-based options representing the mobile sample distribution of the voltage-gated Na+ (Nav) channel, ankyrin G, βIV spectrin, and the cell-adhesion molecule neurofascin have been analyzed, revealing βIV spectrin as essentially the most delicate AIS protein to AKT/GSK3 pathway inhibition.
Inside this pathway, inhibition of AKT by triciribine has the best impact on βIV spectrin localization to the AIS and its subcellular distribution inside neurons, a phenotype that Assist Vector Machine classification was in a position to precisely distinguish from management.
Therapy with triciribine additionally resulted in elevated excitability in main hippocampal neurons. Thus, perturbations to signaling mechanisms throughout the AKT pathway contribute to adjustments in βIV spectrin distribution and neuronal firing that could be related to neuropsychiatric and neurodegenerative problems.
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

Copper publicity induces hepatic G0/G1 cell-cycle arrest by suppressing the Ras/PI3K/Akt signaling pathway in mice

Copper (Cu), as a typical chemical contaminant in surroundings, is understood to be poisonous at excessive concentrations. The present analysis demonstrates the results of copper upon hepatocyte cell-cycle development (CCP) in mice. Institute of most cancers analysis (ICR) mice (n = 240) at an age of 4 weeks have been divided randomly into teams handled with totally different doses of Cu (0, 4, 8, and 16 mg/kg) for 21 and 42 days.

anti- GSK3B antibody

FNab03674 100µg
EUR 505.25
Description: Antibody raised against GSK3B

anti- GSK3B antibody

FNab03675 100µg
EUR 585
Description: Antibody raised against GSK3B

anti- GSK3B antibody

FNab03676 100µg
EUR 548.75
Description: Antibody raised against GSK3B

Anti-GSK3B antibody

PAab03673 100 ug
EUR 355

Anti-GSK3B antibody

PAab03674 100 ug
EUR 355

Anti-GSK3B antibody

PAab03675 100 ug
EUR 412

Anti-GSK3B antibody

PAab03676 100 ug
EUR 386

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-GSK3B Antibody (Biotin)

STJ501296 100 µg
EUR 586

Anti-GSK3B Antibody (FITC)

STJ501297 100 µg
EUR 586

anti-GSK3B (3D10)

LF-MA30340 100 ul
EUR 486
Description: Mouse Monoclonal to GSK3B

Human Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RD-GSK3b-Hu-48Tests 48 Tests
EUR 521

Human Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RD-GSK3b-Hu-96Tests 96 Tests
EUR 723

Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RD-GSK3b-Mu-48Tests 48 Tests
EUR 533

Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RD-GSK3b-Mu-96Tests 96 Tests
EUR 740

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RD-GSK3b-Ra-48Tests 48 Tests
EUR 557

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RD-GSK3b-Ra-96Tests 96 Tests
EUR 775

Human Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

DLR-GSK3b-Hu-48T 48T
EUR 517
Description: A sandwich quantitative ELISA assay kit for detection of Human Glycogen Synthase Kinase 3 Beta (GSK3b) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

DLR-GSK3b-Hu-96T 96T
EUR 673
Description: A sandwich quantitative ELISA assay kit for detection of Human Glycogen Synthase Kinase 3 Beta (GSK3b) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

DLR-GSK3b-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

DLR-GSK3b-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

DLR-GSK3b-Ra-48T 48T
EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glycogen Synthase Kinase 3 Beta (GSK3b) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

DLR-GSK3b-Ra-96T 96T
EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glycogen Synthase Kinase 3 Beta (GSK3b) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RDR-GSK3b-Hu-48Tests 48 Tests
EUR 544

Human Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RDR-GSK3b-Hu-96Tests 96 Tests
EUR 756

Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RDR-GSK3b-Mu-48Tests 48 Tests
EUR 557

Mouse Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RDR-GSK3b-Mu-96Tests 96 Tests
EUR 774

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RDR-GSK3b-Ra-48Tests 48 Tests
EUR 583

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

RDR-GSK3b-Ra-96Tests 96 Tests
EUR 811

Anti-Phospho-GSK3B-(S9) antibody

STJ22160 100 µl
EUR 393
Description: The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Phospho-GSK3B-(Y216) antibody

STJ116370 100 µl
EUR 393
Description: The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Phospho-GSK3B-(Y216) antibody

STJ110852 100 µl
EUR 393
Description: The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-GSK3b (Ab-9) Antibody

A00791-1 100ul
EUR 397
Description: Rabbit Polyclonal GSK3b (Ab-9) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

GSK3B Antibody

1-CSB-PA009038
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

GSK3B Antibody

1-CSB-PA009963GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat, Pig. This antibody is Unconjugated. Tested in the following application: ELISA, WB

GSK3B Antibody

1-CSB-PA009963LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:2000-1:10000, IF:1:50-1:500

GSK3B Antibody

1-CSB-PA002848
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.ELISA:1/20000

GSK3B Antibody

1-CSB-PA080166
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC;WB:1:1000-2000.IHC:1:200-500

GSK3B Antibody

CSB-PA939628-
EUR 335
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

GSK3B Antibody

CSB-PA939628-100ul 100ul
EUR 316
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

GSK3B Antibody

36918-100ul 100ul
EUR 252

GSK3B antibody

38353-100ul 100ul
EUR 252

GSK3B antibody

38616-100ul 100ul
EUR 252

GSK3B Antibody

ABD6806 100 ug
EUR 438

GSK3B Antibody

ABD7231 100 ug
EUR 438

GSK3B Antibody

DF7231 200ul
EUR 304
Description: GSK3B Antibody detects endogenous levels of total GSK3B.

GSK3B Antibody

BF0695 200ul
EUR 376
Description: GSK3B antibody detects endogenous levels of total GSK3B.

GSK3B antibody

10R-1862 100 ul
EUR 403
Description: Mouse monoclonal GSK3B antibody

GSK3B antibody

70R-17615 50 ul
EUR 435
Description: Rabbit polyclonal GSK3B antibody

GSK3b antibody

70R-11869 100 ug
EUR 418
Description: Rabbit polyclonal GSK3b antibody

GSK3B antibody

70R-15005 100 ul
EUR 392
Description: Rabbit polyclonal GSK3B antibody

GSK3A/GSK3B Antibody

1-CSB-PA002838
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3A/GSK3B. Recognizes GSK3A/GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

GSK3B (pY216) Antibody

20-abx123289
  • EUR 495.00
  • EUR 704.00
  • EUR 217.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

GSK3B (pY216) Antibody

20-abx000312
  • EUR 495.00
  • EUR 704.00
  • EUR 217.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

GSK3B Polyclonal Antibody

A62706 100 µg
EUR 570.55
Description: reagents widely cited

GSK3A / GSK3B Antibody

20-abx328487
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

GSK3B Conjugated Antibody

C36918 100ul
EUR 397

GSK3B Conjugated Antibody

C38353 100ul
EUR 397

Phospho-GSK3b antibody

70R-11870 100 ug
EUR 525
Description: Rabbit polyclonal Phospho-GSK3b antibody

Sheep Anti Fitc Polyclonal Antibody,Alk. Phos.

DPBT-68234SF 1 ml
EUR 741

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

GSK3B siRNA

20-abx918789
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GSK3B siRNA

20-abx918790
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GSK3B siRNA

20-abx902344
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

GSK3B Plasmid

PVT7113 2 ug
EUR 266

Phospho-GSK3B (S9) Antibody

1-CSB-PA000536
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-GSK3B (S9). Recognizes Phospho-GSK3B (S9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.ELISA:1/5000

Phospho-GSK3B (S9) Antibody

1-CSB-PA000636
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-GSK3B (S9). Recognizes Phospho-GSK3B (S9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000

GSK3B Antibody, HRP conjugated

1-CSB-PA009963LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GSK3B Antibody, FITC conjugated

1-CSB-PA009963LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GSK3B Antibody, Biotin conjugated

1-CSB-PA009963LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

GSK3B (Ab-9) Antibody

CSB-PA120951-
EUR 335
Description: A polyclonal antibody against GSK3B (Ab-9). Recognizes GSK3B (Ab-9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

GSK3B (Ab-9) Antibody

CSB-PA120951-100ul 100ul
EUR 316
Description: A polyclonal antibody against GSK3B (Ab-9). Recognizes GSK3B (Ab-9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Phospho-GSK3B (Ser9) Antibody

CSB-PA166926-
EUR 335
Description: A polyclonal antibody against Phospho-GSK3B (Ser9). Recognizes Phospho-GSK3B (Ser9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Phospho-GSK3B (Ser9) Antibody

CSB-PA166926-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-GSK3B (Ser9). Recognizes Phospho-GSK3B (Ser9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

GSK3B (pS21 / 29) Antibody

abx031864-400ul 400 ul
EUR 523

GSK3B (pS21 / 29) Antibody

abx031864-80l 80 µl
EUR 286

GSK3B Polyclonal Conjugated Antibody

C30706 100ul
EUR 397

Rabbit Anti-GSK3B monoclonal antibody, clone TZ13-82

DCABH-9320 100 ul
EUR 777

Rabbit Anti-Human GSK3B Polyclonal Antibody, Phospho-Tyr216

CPB-820RH 100 ul
EUR 559

PolyTek, Anti-Rabbit Polymerized Alk-Phos

PAL008 8 ml
EUR 133

PolyTek, Anti-Rabbit Polymerized Alk-Phos

PAL015 15 ml
EUR 197

PolyTek, Anti-Rabbit Polymerized Alk-Phos

PAL125 125 ml
EUR 909

PolyTek, Anti-Rabbit Polymerized Alk-Phos

PAL500 500 ml
EUR 3199

PolyTek, Anti-Rabbit Polymerized Alk-Phos

PAL999 1000 ml
EUR 5776

PolyTek Anti-Mouse Polymerized Alk-Phos

PAT008 8 ml
EUR 133

PolyTek Anti-Mouse Polymerized Alk-Phos

PAT015 15 ml
EUR 197

PolyTek Anti-Mouse Polymerized Alk-Phos

PAT125 125 ml
EUR 909

PolyTek Anti-Mouse Polymerized Alk-Phos

PAT500 500 ml
EUR 3199

PolyTek Anti-Mouse Polymerized Alk-Phos

PAT999 1000 ml
EUR 5776

Rabbit anti Bovine IgG (Alk Phos)

43C-CB0121 1 mg
EUR 399
Description: Rabbit anti Bovine IgG secondary antibody (Alk Phos)

Rabbit anti Bovine IgG (Alk Phos)

43C-CB0135 1 mg
EUR 375
Description: Rabbit anti Bovine IgG secondary antibody (Alk Phos)

Goat anti Bovine IgG (Alk Phos)

43C-CB0142 1 mg
EUR 369
Description: Goat anti Bovine IgG secondary antibody (Alk Phos)

Goat anti Cat IgG (Alk Phos)

43C-CB0221 1 mg
EUR 396
Description: Goat anti Cat IgG secondary antibody (Alk Phos)

Rabbit anti Cat IgG (Alk Phos)

43C-CB0228 1 mg
EUR 382
Description: Rabbit anti Cat IgG secondary antibody (Alk Phos)

Goat anti Cat IgG (Alk Phos)

43C-CB0235 1 mg
EUR 378
Description: Goat anti Cat IgG secondary antibody (Alk Phos)

Rabbit anti Chicken IgG (Alk Phos)

43C-CB0321 1 mg
EUR 399
Description: Rabbit anti Chicken IgG secondary antibody (Alk Phos)

Rabbit anti Chicken IgG (Alk Phos)

43C-CB0328 1 mg
EUR 395
Description: Rabbit anti Chicken IgG secondary antibody (Alk Phos)

Rabbit anti Dog IgG (Alk Phos)

43C-CB0414 1 mg
EUR 399
Description: Rabbit anti Dog IgG secondary antibody (Alk Phos)

Rabbit anti Dog IgG (Alk Phos)

43C-CB0421 1 mg
EUR 382
Description: Rabbit anti Dog IgG secondary antibody (Alk Phos)

Rabbit anti Goat IgG (Alk Phos)

43C-CB0528 1 mg
EUR 382
Description: Rabbit anti Goat IgG secondary antibody (Alk Phos)

Rabbit anti Goat IgG (Alk Phos)

43C-CB0535 1 mg
EUR 375
Description: Rabbit anti Goat IgG secondary antibody (Alk Phos)

Rabbit anti Goat IgG (Alk Phos)

43C-CB0542 500 µg
EUR 392
Description: Rabbit anti Goat IgG secondary antibody (Alk Phos)

Rabbit anti Human IgG (Alk Phos)

43C-CB0949 1 mg
EUR 439
Description: Rabbit anti Human IgG secondary antibody (Alk Phos)

Rabbit anti Human IgG (Alk Phos)

43C-CB0956 1 mg
EUR 375
Description: Rabbit anti Human IgG secondary antibody (Alk Phos)

Goat anti Human IgG (Alk Phos)

43C-CB0963 1 mg
EUR 361
Description: Goat anti Human IgG secondary antibody (Alk Phos)

Rabbit anti Human IgG (Alk Phos)

43C-CB0970 1 mg
EUR 375
Description: Rabbit anti Human IgG secondary antibody (Alk Phos)

Goat anti Human IgG (Alk Phos)

43C-CB0978 1 mg
EUR 403
Description: Goat anti Human IgG secondary antibody (Alk Phos)

Goat anti Human IgM (Alk Phos)

43C-CB0999 1 mg
EUR 380
Description: Goat anti Human IgM secondary antibody (Alk Phos)

Goat anti Rabbit IgG (Alk Phos)

43C-CB1142 1 mg
EUR 389
Description: Goat anti Rabbit IgG secondary antibody (Alk Phos)

Goat anti Rabbit IgG (Alk Phos)

43C-CB1149 1 mg
EUR 481
Description: Goat anti Rabbit IgG secondary antibody (Alk Phos)

Goat anti Rabbit IgG (Alk Phos)

43C-CB1156 1 mg
EUR 361
Description: Goat anti Rabbit IgG secondary antibody (Alk Phos)

Goat anti Rabbit IgG (Alk Phos)

43C-CB1163 1 mg
EUR 452
Description: Goat anti Rabbit IgG secondary antibody (Alk Phos)

Rabbit anti Rat IgG (Alk Phos)

43C-CB1249 1 mg
EUR 405
Description: Rabbit anti Rat IgG secondary antibody (Alk Phos)

Rabbit anti Rat IgG (Alk Phos)

43C-CB1256 1 mg
EUR 399
Description: Rabbit anti Rat IgG secondary antibody (Alk Phos)

Rabbit anti Rat IgM (Alk Phos)

43C-CB1270 1 mg
EUR 458
Description: Rabbit anti Rat IgM secondary antibody (Alk Phos)

Goat anti Rat IgM (Alk Phos)

43C-CB1277 1 mg
EUR 467
Description: Goat anti Rat IgM secondary antibody (Alk Phos)

Rabbit anti Sheep IgG (Alk Phos)

43C-CB1328 1 mg
EUR 405
Description: Rabbit anti Sheep IgG secondary antibody (Alk Phos)

Rabbit anti Sheep IgG (Alk Phos)

43C-CB1335 500 µg
EUR 470
Description: Rabbit anti Sheep IgG secondary antibody (Alk Phos)

Rabbit anti Sheep IgG (Alk Phos)

43C-CB1342 1 mg
EUR 396
Description: Rabbit anti Sheep IgG secondary antibody (Alk Phos)

Rabbit anti Sheep IgG (Alk Phos)

43C-CB1349 500 µg
EUR 469
Description: Rabbit anti Sheep IgG secondary antibody (Alk Phos)
Outcomes confirmed that top Cu publicity precipitated hepatocellular G0/G1 cell-cycle arrest (CCA) and lowered cell proportion within the G2/M part. G0/G1 CCA occurred with down-regulation (p < 0.05) of Ras, p-PI3K (Tyr458), p-Akt (Thr308), p-forkhead field O3 (FOXO3A) (Ser253), p-glycogen synthase kinase 3-β (GSK3-β) (Ser9), murine double minute 2 (MDM2) protein, and mRNA expression ranges, and up-regulation (p < 0.05) of PTEN, p-p53 (Ser15), p27, p21 protein, and mRNA expression ranges, which subsequently suppressed (p < 0.05) the protein and mRNA expression ranges of CDK2/Four and cyclin E/D. These outcomes point out that Cu publicity suppresses the Ras/PI3K/Akt signaling pathway to scale back the extent of CDK2/Four and cyclin E/D, that are important for the G1-S transition, and eventually causes hepatocytes G0/G1 CCA.

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