Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

The Wnt/β-catenin pathway is a extremely conserved, incessantly mutated developmental and most cancers pathway. Its output is outlined primarily by β-catenin’s phosphorylation- and ubiquitylation-dependent proteasomal degradation, initiated by the multi-protein β-catenin destruction advanced.
The exact mechanisms underlying destruction advanced perform have remained unknown, largely due to the shortage of appropriate in vitro techniques. Right here we describe the in vitro reconstitution of an energetic human β-catenin destruction advanced from purified parts, recapitulating advanced meeting, β-catenin modification, and degradation.
We reveal that AXIN1 polymerization and APC promote β-catenin seize, phosphorylation, and ubiquitylation. APC facilitates β-catenin’s flux by the advanced by limiting ubiquitylation processivity and straight interacts with the SCFβ-TrCP E3 ligase advanced in a β-TrCP-dependent method.
Oncogenic APC truncation variants, though a part of the advanced, are functionally impaired. Nonetheless, even essentially the most severely truncated APC variant promotes β-catenin recruitment. These findings exemplify the ability of biochemical reconstitution to interrogate the molecular mechanisms of Wnt/β-catenin signaling.

A Community Pharmacology Strategy to Examine the Mechanism of Erjing Prescription in Sort 2 Diabetes

Erjing prescription (EJP) was an historical system that was recorded within the Normal Medical Assortment of Royal Benevolence of the Track Dynasty. It has been incessantly used to deal with sort 2 diabetes mellitus (T2DM) within the lengthy historical past of China.
The system consists of Lycium barbarum L. and Polygonatum sibiricum F. Delaroche with a ratio of 1 : 1. This examine aimed to establish the potential results and mechanisms of EJP remedy T2DM. The goal proteins and potential pathways of EJP in T2DM remedy have been investigated by the strategy of community pharmacology and real-time PCR (RT-PCR).
99 diabetes-related proteins have been regulated by 56 bioactive constituents in EJP in 26 sign pathways by Cytoscape dedication. In accordance with GO evaluation, 606 genes entries have been enriched. The PPI community prompt that AKT1, EGF, EGFR, MAPK1, and GSK3β proteins have been core genes. Among the many 26 sign pathways, the PI3K-AKT sign pathway was examined by the RT-PCR.
The expression stage of PI3K p85, AKT1, GSK3β, and Myc mRNA of this pathway was regulated by EJP. The examine based mostly on community pharmacology and RT-PCR evaluation revealed that the blood sugar stage was regulated by EJP by way of regulating the PI3K-AKT sign pathway. Loads of new remedy strategies for T2DM utilizing EJP have been supplied by community pharmacology evaluation.
Acquired resistance to MAPK inhibitors limits the medical efficacy in melanoma remedy. We and others have lately proven that BRAF inhibitor (BRAFi)-resistant melanoma cells can develop a dependency on the therapeutic medication to which they’ve acquired resistance, making a vulnerability for these cells that may probably be exploited in most cancers remedy.
In drug-addicted melanoma cells, it was proven that this induction of cell loss of life was preceded by a particular ERK2-dependent phenotype change; nevertheless, the underlying molecular mechanisms are largely missing.
To extend the molecular understanding of this drug dependency, we utilized a mass spectrometry-based proteomic strategy on BRAFi-resistant BRAFMUT 451Lu cells, during which ERK1, ERK2, and JUNB have been silenced individually utilizing CRISPR-Cas9.
Inactivation of ERK2 and, to a lesser extent, JUNB prevents drug dependancy in these melanoma cells, whereas, conversely, knockout of ERK1 fails to reverse this phenotype, exhibiting a response much like that of management cells.
Our evaluation reveals that ERK2 and JUNB share comparable proteome responses dominated by reactivation of cell division. Importantly, we discover that EMT activation in drug-addicted melanoma cells upon drug withdrawal is affected by silencing ERK2 however not ERK1.
Furthermore, transcription issue (regulator) enrichment reveals that PIR acts as an effector of ERK2 and phosphoproteome evaluation reveals that silencing of ERK2 however not ERK1 results in amplification of GSK3 kinase exercise. Our outcomes depict potential mechanisms of drug dependancy in melanoma, which can present a information for therapeutic methods in drug-resistant melanoma.

Inhibition of AKT Signaling Alters βIV Spectrin Distribution on the AIS and Will increase Neuronal Excitability

The axon preliminary section (AIS) is a extremely regulated subcellular area required for neuronal firing. Adjustments within the AIS protein composition and distribution are a type of structural plasticity, which powerfully regulates neuronal exercise and will underlie a number of neuropsychiatric and neurodegenerative problems.
Regardless of its physiological and pathophysiological relevance, the signaling pathways mediating AIS protein distribution are nonetheless poorly studied. Right here, we used confocal imaging and whole-cell patch clamp electrophysiology in main hippocampal neurons to check how AIS protein composition and neuronal firing diversified in response to chose kinase inhibitors focusing on the AKT/GSK3 pathway, which has beforehand been proven to phosphorylate AIS proteins.
Picture-based options representing the mobile sample distribution of the voltage-gated Na+ (Nav) channel, ankyrin G, βIV spectrin, and the cell-adhesion molecule neurofascin have been analyzed, revealing βIV spectrin as essentially the most delicate AIS protein to AKT/GSK3 pathway inhibition.
Inside this pathway, inhibition of AKT by triciribine has the best impact on βIV spectrin localization to the AIS and its subcellular distribution inside neurons, a phenotype that Assist Vector Machine classification was in a position to precisely distinguish from management.
Therapy with triciribine additionally resulted in elevated excitability in main hippocampal neurons. Thus, perturbations to signaling mechanisms throughout the AKT pathway contribute to adjustments in βIV spectrin distribution and neuronal firing that could be related to neuropsychiatric and neurodegenerative problems.
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation

Copper publicity induces hepatic G0/G1 cell-cycle arrest by suppressing the Ras/PI3K/Akt signaling pathway in mice

Copper (Cu), as a typical chemical contaminant in surroundings, is understood to be poisonous at excessive concentrations. The present analysis demonstrates the results of copper upon hepatocyte cell-cycle development (CCP) in mice. Institute of most cancers analysis (ICR) mice (n = 240) at an age of 4 weeks have been divided randomly into teams handled with totally different doses of Cu (0, 4, 8, and 16 mg/kg) for 21 and 42 days.

GSK3B Antibody

36918-100ul 100ul
EUR 302.4

GSK3B antibody

38353 100ul
EUR 439

GSK3B antibody

38353-100ul 100ul
EUR 302.4

GSK3B antibody

38616 100ul
EUR 439

GSK3B antibody

38616-100ul 100ul
EUR 302.4

GSK3B Antibody

CSB-PA939628- each
EUR 402
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

GSK3B Antibody

CSB-PA939628-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

GSK3B Antibody

E036918 100μg/100μl
EUR 255
Description: Available in various conjugation types.

GSK3B Antibody

E10-30705 100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Antibody

E10-30705T 100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Antibody

E10-30006 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GSK3B Antibody

E19-6806 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GSK3B Antibody

E19-7231 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GSK3B antibody

E39-03673 100ug/100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Antibody

E93174 100ul
EUR 255
Description: Available in various conjugation types.

GSK3B Antibody

E92081 100ul
EUR 255
Description: Available in various conjugation types.

GSK3B Antibody

E220184-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

GSK3B Antibody

E220184-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GSK3B Antibody

BF0695 200ul
EUR 540

GSK3B Antibody

BF0695-100ul 100ul
EUR 350

GSK3B Antibody

BF0695-200ul 200ul
EUR 450

GSK3B Antibody

BF0695-50ul 50ul
EUR 250

GSK3B antibody

CAF50144-100ug 100ug
EUR 312

GSK3B antibody

70R-15005 100 ul
EUR 497
Description: Rabbit polyclonal GSK3B antibody

GSK3B antibody

70R-17615 50 ul
EUR 289
Description: Rabbit polyclonal GSK3B antibody

GSK3b antibody

70R-11869 100 ug
EUR 357
Description: Rabbit polyclonal GSK3b antibody

GSK3B Antibody

1-CSB-PA009038
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

GSK3B Antibody

1-CSB-PA009963GA01HU
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  • 150ul
  • 50ul
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat, Pig. This antibody is Unconjugated. Tested in the following application: ELISA, WB

GSK3B Antibody

1-CSB-PA009963LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:2000-1:10000, IF:1:50-1:500

GSK3B Antibody

1-CSB-PA002848
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.ELISA:1/20000

GSK3B Antibody

1-CSB-PA080166
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC;WB:1:1000-2000.IHC:1:200-500

GSK3B Antibody

GWB-MT481D 50ug Ask for price

Gsk3b Antibody

F44221-0.08ML 0.08 ml
EUR 140.25
Description: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity).

Gsk3b Antibody

F44221-0.4ML 0.4 ml
EUR 322.15
Description: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity).

GSK3B Antibody

F51046-0.08ML 0.08 ml
EUR 140.25
Description: GSK3B is a constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. May also mediate the development of insulin resistance by regulating activation of transcription factors. [UniProt]

GSK3B Antibody

F51046-0.4ML 0.4 ml
EUR 322.15
Description: GSK3B is a constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. May also mediate the development of insulin resistance by regulating activation of transcription factors. [UniProt]

Phospho-GSK3A/GSK3B (Y279/216) Antibody

1-CSB-PA000535
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-GSK3A/GSK3B (Y279/216). Recognizes Phospho-GSK3A/GSK3B (Y279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

Rabbit Anti-Human GSK3B Polyclonal Antibody, Phospho-Tyr216

CPB-820RH 100 ul
EUR 670.8

Phospho-GSK3A/GSK3B (Tyr279/216) Antibody

CSB-PA575559- each
EUR 402
Description: A polyclonal antibody against Phospho-GSK3A/GSK3B (Tyr279/216). Recognizes Phospho-GSK3A/GSK3B (Tyr279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Phospho-GSK3A/GSK3B (Tyr279/216) Antibody

CSB-PA575559-100ul 100ul
EUR 434.4
Description: A polyclonal antibody against Phospho-GSK3A/GSK3B (Tyr279/216). Recognizes Phospho-GSK3A/GSK3B (Tyr279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

GSK3A / GSK3B Antibody

20-abx328487
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  • 100 ug
  • 50 ug

GSK3A/GSK3B Antibody

1-CSB-PA002838
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3A/GSK3B. Recognizes GSK3A/GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

GSK3A / GSK3B Antibody

abx328487-100g 100 µg
EUR 250

GSK3A / GSK3B Antibody

abx328487-50g 50 µg
EUR 187.5

Mouse anti GSK3b(pS9) Monoclonal Antibody

TA354463 100 µg Ask for price

GSK3B (pY216) Antibody

20-abx123289
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

GSK3B (pY216) Antibody

20-abx000312
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

GSK3B (pY216) Antibody

abx123289-100l 100 µl
EUR 200

GSK3B (pY216) Antibody

abx123289-1ml 1 ml
EUR 475

GSK3B (pY216) Antibody

abx123289-200l 200 µl
EUR 325

GSK3B (pSer9) Antibody

abx243027-96tests 96 tests
EUR 287.5

Rabbit anti GSK3b(pS9) Polyclonal Antibody

TA354486 100 µg Ask for price

OAGA08182-100UL - GSK3B Antibody (Phospho-Ser9)

OAGA08182-100UL 100ul
EUR 369

OASG03245-100UL - GSK3B Antibody (Phospho-Ser9)

OASG03245-100UL 100ul
EUR 239

OASG03246-100UL - GSK3B Antibody (Phospho-Ser9)

OASG03246-100UL 100ul
EUR 239

OAEC00270-50UG - GSK3B Antibody (Phospho-Tyr216)

OAEC00270-50UG 50ug
EUR 369

GSK3B Polyclonal Antibody

A62706
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  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

GSK3B Polyclonal Antibody

E911360 100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Polyclonal Antibody

E911578 100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Polyclonal Antibody

E916868 100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Polyclonal Antibody

E96164 100ul
EUR 225
Description: Available in various conjugation types.

GSK3B Conjugated Antibody

C36918 100ul
EUR 476.4

GSK3B Conjugated Antibody

C38353 100ul
EUR 476.4

GSK3B Polyclonal Antibody

A72446 50 μl
EUR 408

GSK3B Polyclonal Antibody

A72447 50 μl
EUR 408

GSK3B Polyclonal Antibody

A72448 50 μl
EUR 408

GSK3B Polyclonal Antibody

A72450 50 μl
EUR 557

GSK3 beta Antibody / GSK3B

F54007-0.1ML 0.1 ml
EUR 322.15
Description: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. [UniProt]

GSK3B (Ab-9) Antibody

CSB-PA120951- each
EUR 402
Description: A polyclonal antibody against GSK3B (Ab-9). Recognizes GSK3B (Ab-9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

GSK3B (Ab-9) Antibody

CSB-PA120951-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against GSK3B (Ab-9). Recognizes GSK3B (Ab-9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Anti-GSK3 beta/GSK3B Antibody Picoband™

A00791-3 10ug
EUR 154
Description: Western blot, 0.25-0.5 μg/ml, Human;_x000D_Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human;_x000D_Direct ELISA, 0.1-0.5 μg/ml, Human

GSK3B (pS21 / 29) Antibody

abx031864-400ul 400 ul
EUR 627.6

GSK3B (pS21 / 29) Antibody

abx031864-80l 80 µl
EUR 343.2

GSK3B (pS21 / 29) Antibody

abx031864-400l 400 µl
EUR 525

Rabbit Anti-Human GSK3B (aa 214-218) Polyclonal Antibody

CPB-1178RH 100 ul
EUR 552

Rabbit anti GSK3b(Paired S9) Polyclonal Antibody

TA354454 100 µg Ask for price

GSK3A / GSK3B (pY279 / 216) Antibody

abx333212-100ul 100 ul
EUR 560.4

GSK3A / GSK3B (pY279 / 216) Antibody

20-abx328488
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  • 100 ug
  • 50 ug

GSK3A / GSK3B (pY279 / 216) Antibody

abx333212-100g 100 µg Ask for price

GSK3A / GSK3B (pY279 / 216) Antibody

abx333212-20g 20 µg
EUR 412.5

GSK3A / GSK3B (pY279 / 216) Antibody

abx333212-50g 50 µg Ask for price

GSK3A / GSK3B (pY279 / 216) Antibody

abx328488-100g 100 µg
EUR 250

GSK3A / GSK3B (pY279 / 216) Antibody

abx328488-50g 50 µg
EUR 187.5

GSK3B Antibody, HRP conjugated

1-CSB-PA009963LB01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GSK3B Antibody, FITC conjugated

1-CSB-PA009963LC01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

OAPA00251-5UG - GSK3B Antibody

OAPA00251-5UG 5ug
EUR 69

OACA03072-50UG - GSK3B Antibody

OACA03072-50UG 50ug
EUR 179

GSK3B Rabbit Polyclonal Antibody

53236 100ul
EUR 439

GSK3B Antibody, Biotin conjugated

1-CSB-PA009963LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OASG03249-100UL - GSK3B Antibody

OASG03249-100UL 100ul
EUR 239

OASG07740-100UL - GSK3B Antibody

OASG07740-100UL 100ul
EUR 269

OASG07741-100UL - GSK3B Antibody

OASG07741-100UL 100ul
EUR 269

OASG07742-100UL - GSK3B Antibody

OASG07742-100UL 100ul
EUR 269

OAAJ02835-100UL - GSK3B Antibody

OAAJ02835-100UL 100ul
EUR 319

OAAJ07280-100UL - GSK3B Antibody

OAAJ07280-100UL 100uL
EUR 319

OAAF01318-100UG - GSK3B Antibody

OAAF01318-100UG 100ug
EUR 329

OACA03072-100UG - GSK3B Antibody

OACA03072-100UG 100ug
EUR 319

GSK3B Rabbit Polyclonal Antibody

E312222 100ug/200ul
EUR 295
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GSK3B Rabbit Polyclonal Antibody

E10G10937 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GSK3B Rabbit Polyclonal Antibody

E10G12655 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GSK3B Rabbit Polyclonal Antibody

E10G05782 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GSK3A/GSK3B (pY216 + pY279) Antibody

abx243004-96tests 96 tests
EUR 287.5

Polyclonal GSK3B / GSK3 Beta Antibody

AMM05221G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GSK3B / GSK3 Beta . This antibody is tested and proven to work in the following applications:

GSK3B Polyclonal Conjugated Antibody

C30706 100ul
EUR 476.4

GSK3A / GSK3B (Ab-279 / 216) Antibody

abx332166-100ul 100 ul
EUR 510

GSK3A/GSK3B (Ab-279/216) Antibody

CSB-PA255909- each
EUR 402
Description: A polyclonal antibody against GSK3A/GSK3B (Ab-279/216). Recognizes GSK3A/GSK3B (Ab-279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

GSK3A/GSK3B (Ab-279/216) Antibody

CSB-PA255909-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against GSK3A/GSK3B (Ab-279/216). Recognizes GSK3A/GSK3B (Ab-279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Antibody for Human GSK3b (pSer9)

SPC-1384D-A565 0.1ml
EUR 480
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 565.

Antibody for Human GSK3b (pSer9)

SPC-1384D-A633 0.1ml
EUR 480
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 633.

Antibody for Human GSK3b (pSer9)

SPC-1384D-A655 0.1ml
EUR 480
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 655.

Antibody for Human GSK3b (pSer9)

SPC-1384D-A680 0.1ml
EUR 480
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 680.

Antibody for Human GSK3b (pSer9)

SPC-1384D-A700 0.1ml
EUR 480
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 700.

Antibody for Human GSK3b (pSer9)

SPC-1384D-ALP 0.1ml
EUR 472.8
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Alkaline Phosphatase.

Antibody for Human GSK3b (pSer9)

SPC-1384D-APC 0.1ml
EUR 478.8
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to APC .

Antibody for Human GSK3b (pSer9)

SPC-1384D-APCCY7 0.1ml
EUR 565.2
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to APC/Cy7.

Antibody for Human GSK3b (pSer9)

SPC-1384D-DY350 0.1ml
EUR 496.8
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 350.

Antibody for Human GSK3b (pSer9)

SPC-1384D-DY405 0.1ml
EUR 483.6
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 405.

Antibody for Human GSK3b (pSer9)

SPC-1384D-DY488 0.1ml
EUR 471.6
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 488.

Antibody for Human GSK3b (pSer9)

SPC-1384D-DY594 0.1ml
EUR 474
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 594.

Antibody for Human GSK3b (pSer9)

SPC-1384D-DY633 0.1ml
EUR 468
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 633.

Antibody for Human GSK3b (pSer9)

SPC-1384D-HRP 0.1ml
EUR 465.6
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to HRP.

Antibody for Human GSK3b (pSer9)

SPC-1384D-P594 0.1ml
EUR 488.4
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to PE/ATTO 594.
Outcomes confirmed that top Cu publicity precipitated hepatocellular G0/G1 cell-cycle arrest (CCA) and lowered cell proportion within the G2/M part. G0/G1 CCA occurred with down-regulation (p < 0.05) of Ras, p-PI3K (Tyr458), p-Akt (Thr308), p-forkhead field O3 (FOXO3A) (Ser253), p-glycogen synthase kinase 3-β (GSK3-β) (Ser9), murine double minute 2 (MDM2) protein, and mRNA expression ranges, and up-regulation (p < 0.05) of PTEN, p-p53 (Ser15), p27, p21 protein, and mRNA expression ranges, which subsequently suppressed (p < 0.05) the protein and mRNA expression ranges of CDK2/Four and cyclin E/D. These outcomes point out that Cu publicity suppresses the Ras/PI3K/Akt signaling pathway to scale back the extent of CDK2/Four and cyclin E/D, that are important for the G1-S transition, and eventually causes hepatocytes G0/G1 CCA.

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