Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production

Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production

Inflammatory bowel ailments (IBD) are prevalent and debilitating ailments; their scientific treatment is desperately unmet. Mesenchymal stem cells (MSCs) are pluripotent stem cells with a number of immunomodulatory results, that are attributed to their efficacy within the IBD rodent mannequin.
Optimization of MSC regimes in IBD is an important step for his or her additional scientific software. Wogonin is a flavonoid-like compound, which confirmed in depth immunomodulatory and adjuvant results. This analysis is aimed toward investigating whether or not and the way Wogonin boosted the therapeutic effectivity of MSCs on DSS-induced colitis.
Our outcomes confirmed that the MSC therapy with Wogonin considerably alleviated the intestinal irritation in IBD mice by elevated IL-10 expression. In vitro experiments, Wogonin clearly raised the IL-10 manufacturing and ROS ranges of MSCs in a dose-dependent method. In the meantime, western blot knowledge advised Wogonin improves the IL-10 manufacturing by inducing transcript issue HIF-1α expression by way of AKT/GSK3β sign pathway.
Lastly, the favorable results of Wogonin on MSCs have been confirmed by IL-10 blockade experiment in vivo. Collectively, our outcomes advised that Wogonin considerably elevated the IL-10 manufacturing and enhanced the therapeutic results of MSCs in DSS-induced colitis. This work advised Wogonin as a novel optimum technique for MSC scientific software.
 Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production

SUMO conjugation susceptibility of Akt/protein kinase B impacts the expression of the pluripotency transcription issue Nanog in embryonic stem cells

Akt/PKB is a kinase concerned within the regulation of all kinds of cell processes. Its exercise is modulated by various post-translational modifications (PTMs). Significantly, conjugation of the small ubiquitin-related modifier (SUMO) to this kinase impacts on a number of mobile features, similar to proliferation and splicing. In embryonic stem (ES) cells, this kinase is vital for pluripotency upkeep.
Amongst different features, Akt is understood to advertise the expression of Nanog, a central pluripotency transcription issue (TF). Nonetheless, the relevance of this particular PTM of Akt has not been beforehand analyzed on this context. On this work, we examine the impact of Akt1 variants with differential SUMOylation susceptibility on the expression of Nanog.
Our outcomes exhibit that each, the Akt1 functionality of being modified by SUMO conjugation and a useful SUMO conjugase exercise are required to induce Nanog gene expression. Likewise, we discovered that the frequent oncogenic E17Okay Akt1 mutant affected Nanog expression in ES cells additionally in a SUMOylatability dependent method.
Curiously, this final result takes locations in ES cells however not in a non-pluripotent heterologous system, suggesting the presence of an important issue for this induction in ES cells. Remarkably, the 2 main candidate components to mediate this induction, GSK3-β and Tbx3, are non-essential gamers of this impact, suggesting a posh mechanism in all probability involving non-canonical pathways.
Moreover, we discovered that Akt1 subcellular distribution doesn’t depend upon its SUMOylatability, indicating that Akt localization has no affect on the impact on Nanog, and that apart from the membrane localization of E17Okay Akt mutant, SUMOylation can also be required for its hyperactivity.
Our outcomes spotlight the influence of SUMO conjugation within the operate of a kinase related for a plethora of mobile processes, together with the management of a key pluripotency TF.
 Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production

Results of graphene on morphology, microstructure and transcriptomic profiling of Pinus tabuliformis Carr. roots

Graphene has proven nice potential for bettering progress of many vegetation, however its impact on woody vegetation stays basically unstudied. On this work, Pinus tabuliformis Carr. bare-rooted seedlings grown open air in pots have been irrigated with a graphene resolution over a focus vary of 0-50 mg/L for six months. Graphene was discovered to stimulate root progress, with a maximal impact at 25 mg/L.
We then investigated root microstructure and carried out transcript profiling of root supplies handled with Zero and 25 mg/L graphene. Graphene therapy resulted in plasma-wall separation and destruction of membrane integrity in root cells.
Greater than 50 thousand of differentially expressed genes (DEGs) have been obtained by RNA sequencing, amongst which 6477 may very well be annotated utilizing different plant databases. The GO enrichment evaluation and KEGG pathway evaluation of the annotated DEGs indicated that abiotic stress responses, which resemble salt stress, have been induced by graphene therapy in roots, whereas responses to biotic stimuli have been inhibited.
Quite a few metabolic processes and hormone sign transduction pathways have been altered by the therapy. The expansion promotion results of graphene could also be mediated by encouraging proline synthesis, and suppression of the expression of the auxin response gene SMALL AUXIN UP-REGULATED RNA 41 (SAUR41), PYL genes which encode ABA receptors, and GSK3 homologs.

3-hydroxyanthranic acid will increase the sensitivity of hepatocellular carcinoma to sorafenib by reducing tumor cell stemness

Sorafenib is the FDA-approved first-line goal drug for HCC sufferers. Nonetheless, sorafenib solely confers 3-5 months of survival profit with <30% of HCC sufferers. Thus, it’s essential to develop a sensitizer for hepatocellular carcinoma (HCC) to sorafenib.
Right here, we report that in consultant HCC cell strains (SMMC-7721 and PLC8024) which can be insensitive to sorafenib, 3-HAA (50 μM) considerably enhances cell sensitivity to sorafenib to an extent that would not be defined by additive results. In nude mice carrying HCC xenograft, tumor progress is inhibited by sorafenib (10 mg/kg/day) or 3-HAA (100 mg/kg/day) alone. When utilized in mixture, the therapy successfully prevents the xenograft from rising.
In a set of mechanistic experiments, we discover enhanced AKT activation and elevated proportion of CD44+CD133+ cells in sorafenib-resistant HCC cells and tissues. The proportion of CD44+CD133+ cells is decreased upon 3-HAA therapy in each cultured cells and mouse xenografts, suggesting that 3-HAA may lower the stemness of HCC. We additionally detect decreased phosphorylation of AKT, a regulator of the GSK3β/β-catenin signaling upon 3-HAA therapy.

HSPC210 (GSKIP) (NM_016472) Human Over-expression Lysate

LS051527 100ug
EUR 628
Description: Transient overexpression lysate of chromosome 14 open reading frame 129 (C14orf129)

Human GSK3A Protein Lysate 20ug

IHUGSK3APLLY20UG each
EUR 213
Description: Human GSK3A Protein Lysate 20ug

OCOA09277-20UG - GSK3A Protein Lysate

OCOA09277-20UG 20ug
EUR 169

Protein expression

CRO-4-Ind each Ask for price

EP Reagent Biuret Reagent - 100ML

1011601 1L
EUR 59.4

RFP expression Adenovirus, in vivo ready

AVP001-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made RFP expression adenovirus, provided in PBS solution.

CFP expression Adenovirus, in vivo ready

AVP002-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made CFP expression adenovirus, provided in PBS solution.

GFP expression Adenovirus, in vivo ready

AVP011-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made GFP expression adenovirus, provided in PBS solution.

YFP expression Adenovirus, in vivo ready

AVP012-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made YFP expression adenovirus, provided in PBS solution.

BFP expression Adenovirus, in vivo ready

AVP017-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made BFP expression adenovirus, provided in PBS solution.

YLEX Expression Kit

FYY201-1KT 1 kit Ask for price

PEROXIDE BLOCKING REAGENT Reagent

GWB-Q00308 50 ml Ask for price

EP Reagent Iodoplatinate Reagent - 200ML

1046300 200ML
EUR 502.2

EP Reagent Molybdovanadic Reagent - 100ML

1056700 100ML
EUR 59.4

h OCT4 expression Adenovirus, in vivo ready

AVP013-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.

h SOX2 expression Adenovirus, in vivo ready

AVP014-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.

293 Expression MAX-1

EXP-711-11 1ml
EUR 214
Description: 293 Expression MAX-1 is expressed from - -.

EP Reagent Methoxyphenylacetic Reagent - 100ML

1053601 100ML
EUR 490.05

h LIN28 expression Adenovirus, in vivo ready

AVP015-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.

CNGA2 Expression vector

60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

CNGA2 Expression vector

GWB-PSFA61 20ug Ask for price

LEXSYcon2.1 Expression Kit

EGE-1310ble 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310bsd 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310hyg 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310neo 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310pac 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310sat 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

EP Reagent Phosphomolybdotungstic Reagent - 100ML

1065000 100ML
EUR 226.8

Blunt E1 Expression Kit

abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit

abx098873-10rxns 10 rxns
EUR 560.4

Blunt E1 Expression Kit

abx098098-100l 100 µl
EUR 712.5

Blunt E1 Expression Kit

abx098098-1ml 1 ml Ask for price

Blunt E1 Expression Kit

abx098098-200l 200 µl Ask for price

Blunt E2 Expression Kit

abx098873-100l 100 µl
EUR 712.5

Blunt E2 Expression Kit

abx098873-1ml 1 ml Ask for price

Blunt E2 Expression Kit

abx098873-200l 200 µl Ask for price

RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus

AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus

AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

AVIDIN/BIOTIN BLOCKING REAGENT Reagent

GWB-Q00289 30 ml Ask for price

Gordon-McLeod Reagent (Oxidase reagent)

R026-100ML 1 unit
EUR 7.76
Description: Gordon-McLeod Reagent (Oxidase reagent)

LEXSinduce3 Expression Kit

EGE-1410ble 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce3 Expression Kit

EGE-1410blecherry 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce3 Expression Kit

EGE-1410neo 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce4 Expression Kit

EGE-1420blecherry 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

Oxidase Reagent (Gordon-Macleod Reagent)

76207 100 ml
EUR 3.38
Description: Part B

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

NEU3 Over-expression Lysate

GWB-DL2468 0.1 mg Ask for price

EP Reagent Sulfomolybdic Reagent R3 - 1L

1086500 1L
EUR 398.25

TRIpure Reagent (same as Trizol Reagent)

MBS356001-INQUIRE INQUIRE Ask for price

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

OPN expression inhibitor 1

T62820-10mg 10mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-1g 1g Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-1mg 1mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-50mg 50mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-5mg 5mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

HY-146064 10 mg
EUR 2272.76
Description: OPN expression inhibitor 1 (Compound 11) is an osteopontin (OPN) expression inhibitor. OPN expression inhibitor 1 can be used for breast cancer research[1].

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 530

h IL6 Expression Lentivirus

LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus

LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus

LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus

LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus

LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.

h FAS Expression Lentivirus

LVP1181 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.

h ADA Expression Lentivirus

LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h FAP Expression Lentivirus

LVP1307 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FAP, containing a RFP-Blasticidin dual selection marker.

h CD1 Expression Lentivirus

LVP1320 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CD1, containing a RFP-Blasticidin dual selection marker.

m FAP Expression Lentivirus

LVP1326 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: FAP, containing a RFP-Blasticidin dual selection marker.

h VIM Expression Lentivirus

LVP1357 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: VIM, containing a RFP-Blasticidin dual selection marker.

h PXN Expression Lentivirus

LVP1358 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PXN, containing a RFP-Blasticidin dual selection marker.

m CD8 Expression Lentivirus

LVP1363 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: CD8, containing a RFP-Blasticidin dual selection marker.

h TPO Expression Lentivirus

LVP1371 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: TPO, containing a RFP-Blasticidin dual selection marker.

h WAS Expression Lentivirus

LVP1429 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: WAS, containing a RFP-Blasticidin dual selection marker.

m PGR Expression Lentivirus

LVP1455 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for mouse target: PGR containing a RFP-Blasticidin dual selection marker.

h MAG Expression Lentivirus

LVP1485 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: MAG, containing a RFP-Blasticidin dual selection marker.

h FST Expression Lentivirus

LVP1518 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human FST gene, containing a RFP-Blasticidin dual selection marker.

pAAV-MCS Expression Vector

MBS169528-001mg 0.01mg
EUR 695

pAAV-MCS Expression Vector

MBS169528-5x001mg 5x0.01mg
EUR 2955

Total RNA Isolation Reagent (Reagent Based)

MBS402052-100mL 100mL
EUR 235

Total RNA Isolation Reagent (Reagent Based)

MBS402052-4Reactions 4Reactions
EUR 165

FlashBAC - Baculovirus Expression System (96 reactions)

GWB-6C34E3 Kit Ask for price

FlashBAC - Baculovirus Expression System (5 reactions)

GWB-7676BB Kit Ask for price

FlashBAC - Baculovirus Expression System(24 reactions)

GWB-EB1FF8 Kit Ask for price

h OCT4 expression Adenovirus

AVP013 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.

h SOX2 expression Adenovirus

AVP014 1x109 IFU/ml x 50ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.

h EGFR Expression Lentivirus

LVP1142 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker.

h NRP1 Expression Lentivirus

LVP1145 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h MUC1 Expression Lentivirus

LVP1148 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD8a Expression Lentivirus

LVP1151 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker.

h PIGF Expression Lentivirus

LVP1154 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker.

h BRAF Expression Lentivirus

LVP1156 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker.

h ROS1 Expression Lentivirus

LVP1158 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker.

h CD3E Expression Lentivirus

LVP1163 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KLK3 Expression Lentivirus

LVP1171 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker.

h NEK6 Expression Lentivirus

LVP1182 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker.

h NEK7 Expression Lentivirus

LVP1183 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker.

h CDR1 Expression Lentivirus

LVP917 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.

h CD47 Expression Lentivirus

LVP918 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.

h ESR1 Expression Lentivirus

LVP924 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker.

r Syn1 Expression Lentivirus

LVP925 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker.

h NME4 Expression Lentivirus

LVP932 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker.

h STM2 Expression Lentivirus

LVP1210 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Pre-made over-expression lentivirus for human target: STM2, containing a RFP-Blasticidin dual selection marker.

h CBX7 Expression Lentivirus

LVP1308 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CBX7, containing a RFP-Blasticidin dual selection marker.

h ACE2 Expression Lentivirus

LVP1310 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ACE2, containing a RFP-Blasticidin dual selection marker.

h Rab7 Expression Lentivirus

LVP1315 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Rab7, containing a RFP-Blasticidin dual selection marker.

h Rab5 Expression Lentivirus

LVP1316 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Rab5, containing a RFP-Blasticidin dual selection marker.

m ASPA Expression Lentivirus

LVP1317 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: ASPA, containing a RFP-Blasticidin dual selection marker.

h CD44 Expression Lentivirus

LVP1318 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CD44, containing a RFP-Blasticidin dual selection marker.

h MFN2 Expression Lentivirus

LVP1327 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: MFN2, containing a RFP-Blasticidin dual selection marker.

h ANO1 Expression Lentivirus

LVP1344 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ANO1 containing a RFP-Blasticidin dual selection marker.

h FGL2 Expression Lentivirus

LVP1346 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FGL2, containing a RFP-Blasticidin dual selection marker.

h CCR2 Expression Lentivirus

LVP1354 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CCR2, containing a RFP-Blasticidin dual selection marker.

h NEFL Expression Lentivirus

LVP1360 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NEFL, containing a RFP-Blasticidin dual selection marker.

h NOS2 Expression Lentivirus

LVP1367 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NOS2, containing a RFP-Blasticidin dual selection marker.

h BMI1 Expression Lentivirus

LVP1372 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: BMI1, containing a RFP-Blasticidin dual selection marker.

h PSCA Expression Lentivirus

LVP1374 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PSCA, containing a RFP-Blasticidin dual selection marker.

h POLQ Expression Lentivirus

LVP1421 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: POLQ containing a RFP-Blasticidin dual selection marker.

h ARSA Expression Lentivirus

LVP1425 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ARSA, containing a RFP-Blasticidin dual selection marker.

h IDUA Expression Lentivirus

LVP1426 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: IDUA, containing a RFP-Blasticidin dual selection marker.

h SGSH Expression Lentivirus

LVP1427 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: SGSH, containing a RFP-Blasticidin dual selection marker.

h CYBB Expression Lentivirus

LVP1430 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CYBB, containing a RFP-Blasticidin dual selection marker.

h CAV1 Expression Lentivirus

LVP1431 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CAV1, containing a RFP-Blasticidin dual selection marker.

m INS2 Expression Lentivirus

LVP1456 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for mouse target: INS2 containing a RFP-Blasticidin dual selection marker.

h ROR1 Expression Lentivirus

LVP1458 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: ROR1, containing a RFP-Blasticidin dual selection marker.

h DGKA Expression Lentivirus

LVP1468 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: DGKA, containing a RFP-Blasticidin dual selection marker.

h REST_HA Expression Lentivirus

LVP1475 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: REST, containing a RFP-Blasticidin dual selection marker.

h UBR7 Expression Lentivirus

LVP1483 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: UBR7, containing a RFP-Blasticidin dual selection marker.

h MMP1 Expression Lentivirus

LVP1516 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human MMP1 gene, containing a RFP-Blasticidin dual selection marker.

h BDNF Expression Lentivirus

LVP1520 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human BDNF gene, containing a RFP-Blasticidin dual selection marker.

m CCL5 Expression Lentivirus

LVP1619 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express mouse CCL5 gene, containing a RFP-Blasticidin dual selection marker.

Genomic DNA Isolation Reagent (Reagent Based)

MBS402050-100mL 100mL
EUR 255

Genomic DNA Isolation Reagent (Reagent Based)

MBS402050-4Reactions 4Reactions
EUR 185

h LIN28 expression Adenovirus

AVP015 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

h HDAC1 Expression Lentivirus

LVP1143 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h VEGFB Expression Lentivirus

LVP1146 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h PARP1 Expression Lentivirus

LVP1149 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h IL3RA Expression Lentivirus

LVP1150 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.

h PTCH1 Expression Lentivirus

LVP1155 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker.

h SART3 Expression Lentivirus

LVP1159 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker.

h TIGIT Expression Lentivirus

LVP1160 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker.

h EDNRA Expression Lentivirus

LVP1165 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker.

h Foxp3 Expression Lentivirus

LVP1172 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker.

m RIKEN Expression Lentivirus

LVP1174 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker.

h APLP2 Expression Lentivirus

LVP1177 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker.

h PCSK9 Expression Lentivirus

LVP1178 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker.

h NTSR1 Expression Lentivirus

LVP1179 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker.

h GPNMB Expression Lentivirus

LVP921 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker.

h DECR1 Expression Lentivirus

LVP927 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker.

h DECR2 Expression Lentivirus

LVP928 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker.

h NMRK1 Expression Lentivirus

LVP930 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker.

h PITX3 Expression Lentivirus

LVP931 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker.

h FADS2 Expression Lentivirus

LVP1319 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FADS2, containing a RFP-Blasticidin dual selection marker.

h OVOL2 Expression Lentivirus

LVP1322 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: OVOL2, containing a RFP-Blasticidin dual selection marker.

h APOA1 Expression Lentivirus

LVP1324 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: APOA1, containing a RFP-Blasticidin dual selection marker.

h SOX10 Expression Lentivirus

LVP1325 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: SOX10, containing a RFP-Blasticidin dual selection marker.

h P2RY1 Expression Lentivirus

LVP1328 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: P2RY1, containing a RFP-Blasticidin dual selection marker.

h TUSC2 Expression Lentivirus

LVP1343 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: TUSC2, containing a RFP-Blasticidin dual selection marker.

h NLRP3 Expression Lentivirus

LVP1350 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NLRP3, containing a RFP-Blasticidin dual selection marker.

h CCL19 Expression Lentivirus

LVP1353 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CCL19, containing a RFP-Blasticidin dual selection marker.

h Sirt2 Expression Lentivirus

LVP1373 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Sirt2, containing a RFP-Blasticidin dual selection marker.

h EPCAM Expression Lentivirus

LVP1375 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: EPCAM, containing a RFP-Blasticidin dual selection marker.

h PDE6B Expression Lentivirus

LVP1423 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PDE6B, containing a RFP-Blasticidin dual selection marker.

h ABCA4 Expression Lentivirus

LVP1424 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ABCA4, containing a RFP-Blasticidin dual selection marker.

h NAGLU Expression Lentivirus

LVP1428 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NAGLU, containing a RFP-Blasticidin dual selection marker.

h ACOD1 Expression Lentivirus

LVP1432 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ACOD1, containing a RFP-Blasticidin dual selection marker.

h SIRPA Expression Lentivirus

LVP1459 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: SIRPA, containing a RFP-Blasticidin dual selection marker.

h RIOK1 Expression Lentivirus

LVP1469 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: RIOK1, containing a RFP-Blasticidin dual selection marker.

h HDAC8 Expression Lentivirus

LVP1482 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: HDAC8, containing a RFP-Blasticidin dual selection marker.

h CD226 Expression Lentivirus

LVP1484 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: CD226, containing a RFP-Blasticidin dual selection marker.

h TAOK1 Expression Lentivirus

LVP1509 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human TAOK1 gene, containing a RFP-Blasticidin dual selection marker.

h CD155 Expression Lentivirus

LVP1512 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human CD155 gene, containing a RFP-Blasticidin dual selection marker.

h GSTA1 Expression Lentivirus

LVP1514 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human GSTA1 gene, containing a RFP-Blasticidin dual selection marker.
The AKT activator SC79 prompts GSK3 β/β-catenin signaling whereas the Wnt inhibitor XAV-939 abolishes 3-HAA inhibition of HCC progress in vitro and in mice. The present examine demonstrates that 3-HAA sensitizes HCC cells to sorafenib by lowering tumor stemness, suggesting it’s a promising molecule for HCC remedy.

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