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Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production
Lieven
- 0
Inflammatory bowel ailments (IBD) are prevalent and debilitating ailments; their scientific treatment is desperately unmet. Mesenchymal stem cells (MSCs) are pluripotent stem cells with a number of immunomodulatory results, that are attributed to their efficacy within the IBD rodent mannequin.
Optimization of MSC regimes in IBD is an important step for his or her additional scientific software. Wogonin is a flavonoid-like compound, which confirmed in depth immunomodulatory and adjuvant results. This analysis is aimed toward investigating whether or not and the way Wogonin boosted the therapeutic effectivity of MSCs on DSS-induced colitis.
Our outcomes confirmed that the MSC therapy with Wogonin considerably alleviated the intestinal irritation in IBD mice by elevated IL-10 expression. In vitro experiments, Wogonin clearly raised the IL-10 manufacturing and ROS ranges of MSCs in a dose-dependent method. In the meantime, western blot knowledge advised Wogonin improves the IL-10 manufacturing by inducing transcript issue HIF-1α expression by way of AKT/GSK3β sign pathway.
Lastly, the favorable results of Wogonin on MSCs have been confirmed by IL-10 blockade experiment in vivo. Collectively, our outcomes advised that Wogonin considerably elevated the IL-10 manufacturing and enhanced the therapeutic results of MSCs in DSS-induced colitis. This work advised Wogonin as a novel optimum technique for MSC scientific software.

SUMO conjugation susceptibility of Akt/protein kinase B impacts the expression of the pluripotency transcription issue Nanog in embryonic stem cells
Akt/PKB is a kinase concerned within the regulation of all kinds of cell processes. Its exercise is modulated by various post-translational modifications (PTMs). Significantly, conjugation of the small ubiquitin-related modifier (SUMO) to this kinase impacts on a number of mobile features, similar to proliferation and splicing. In embryonic stem (ES) cells, this kinase is vital for pluripotency upkeep.
Amongst different features, Akt is understood to advertise the expression of Nanog, a central pluripotency transcription issue (TF). Nonetheless, the relevance of this particular PTM of Akt has not been beforehand analyzed on this context. On this work, we examine the impact of Akt1 variants with differential SUMOylation susceptibility on the expression of Nanog.
Our outcomes exhibit that each, the Akt1 functionality of being modified by SUMO conjugation and a useful SUMO conjugase exercise are required to induce Nanog gene expression. Likewise, we discovered that the frequent oncogenic E17Okay Akt1 mutant affected Nanog expression in ES cells additionally in a SUMOylatability dependent method.
Curiously, this final result takes locations in ES cells however not in a non-pluripotent heterologous system, suggesting the presence of an important issue for this induction in ES cells. Remarkably, the 2 main candidate components to mediate this induction, GSK3-β and Tbx3, are non-essential gamers of this impact, suggesting a posh mechanism in all probability involving non-canonical pathways.
Moreover, we discovered that Akt1 subcellular distribution doesn’t depend upon its SUMOylatability, indicating that Akt localization has no affect on the impact on Nanog, and that apart from the membrane localization of E17Okay Akt mutant, SUMOylation can also be required for its hyperactivity.
Our outcomes spotlight the influence of SUMO conjugation within the operate of a kinase related for a plethora of mobile processes, together with the management of a key pluripotency TF.

Results of graphene on morphology, microstructure and transcriptomic profiling of Pinus tabuliformis Carr. roots
Graphene has proven nice potential for bettering progress of many vegetation, however its impact on woody vegetation stays basically unstudied. On this work, Pinus tabuliformis Carr. bare-rooted seedlings grown open air in pots have been irrigated with a graphene resolution over a focus vary of 0-50 mg/L for six months. Graphene was discovered to stimulate root progress, with a maximal impact at 25 mg/L.
We then investigated root microstructure and carried out transcript profiling of root supplies handled with Zero and 25 mg/L graphene. Graphene therapy resulted in plasma-wall separation and destruction of membrane integrity in root cells.
Greater than 50 thousand of differentially expressed genes (DEGs) have been obtained by RNA sequencing, amongst which 6477 may very well be annotated utilizing different plant databases. The GO enrichment evaluation and KEGG pathway evaluation of the annotated DEGs indicated that abiotic stress responses, which resemble salt stress, have been induced by graphene therapy in roots, whereas responses to biotic stimuli have been inhibited.
Quite a few metabolic processes and hormone sign transduction pathways have been altered by the therapy. The expansion promotion results of graphene could also be mediated by encouraging proline synthesis, and suppression of the expression of the auxin response gene SMALL AUXIN UP-REGULATED RNA 41 (SAUR41), PYL genes which encode ABA receptors, and GSK3 homologs.
3-hydroxyanthranic acid will increase the sensitivity of hepatocellular carcinoma to sorafenib by reducing tumor cell stemness
Sorafenib is the FDA-approved first-line goal drug for HCC sufferers. Nonetheless, sorafenib solely confers 3-5 months of survival profit with <30% of HCC sufferers. Thus, it’s essential to develop a sensitizer for hepatocellular carcinoma (HCC) to sorafenib.
Right here, we report that in consultant HCC cell strains (SMMC-7721 and PLC8024) which can be insensitive to sorafenib, 3-HAA (50 μM) considerably enhances cell sensitivity to sorafenib to an extent that would not be defined by additive results. In nude mice carrying HCC xenograft, tumor progress is inhibited by sorafenib (10 mg/kg/day) or 3-HAA (100 mg/kg/day) alone. When utilized in mixture, the therapy successfully prevents the xenograft from rising.
In a set of mechanistic experiments, we discover enhanced AKT activation and elevated proportion of CD44+CD133+ cells in sorafenib-resistant HCC cells and tissues. The proportion of CD44+CD133+ cells is decreased upon 3-HAA therapy in each cultured cells and mouse xenografts, suggesting that 3-HAA may lower the stemness of HCC. We additionally detect decreased phosphorylation of AKT, a regulator of the GSK3β/β-catenin signaling upon 3-HAA therapy.
![]() Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 478 |
![]() Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 662 |
![]() Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
![]() Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
![]() HEK-293T Telomerase Over-Expressing Cell Pellet |
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abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
![]() Gsk3a/ Rat Gsk3a ELISA Kit |
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ELI-02619r | Lifescience Market | 96 Tests | EUR 886 |
![]() GSK3A siRNA |
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20-abx918787 | Abbexa |
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![]() GSK3A siRNA |
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20-abx918788 | Abbexa |
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![]() GSK3A Antibody |
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36917-100ul | SAB | 100ul | EUR 252 |
![]() GSK3A siRNA |
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20-abx902343 | Abbexa |
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![]() GSK3A Antibody |
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1-CSB-PA05369A0Rb | Cusabio |
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Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
![]() GSK3A Antibody |
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1-CSB-PA147596 | Cusabio |
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Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200 |
![]() GSK3A Antibody |
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CSB-PA205981- | Cusabio | EUR 335 | |
Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200 |
![]() GSK3A Antibody |
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CSB-PA205981-100ul | Cusabio | 100ul | EUR 314 |
Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200 |
![]() GSK3A Antibody |
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1-CSB-PA009962GA01HU | Cusabio |
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Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
![]() GSK3A Antibody |
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1-CSB-PA932427 | Cusabio |
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Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200 |
![]() GSK3A Antibody |
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1-CSB-PA002836 | Cusabio |
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Description: A polyclonal antibody against GSK3A. Recognizes GSK3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.ELISA:1/20000 |
![]() GSK3A Antibody |
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43681-100ul | SAB | 100ul | EUR 252 |
![]() GSK3A antibody |
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70R-17614 | Fitzgerald | 50 ul | EUR 435 |
Description: Rabbit polyclonal GSK3A antibody |
![]() GSK3A antibody |
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20R-1287 | Fitzgerald | 100 ug | EUR 377 |
Description: Rabbit polyclonal GSK3A antibody |
![]() GSK3a antibody |
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70R-11868 | Fitzgerald | 100 ug | EUR 447 |
Description: Rabbit polyclonal GSK3a antibody |
![]() Bradford reagent |
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BDE641 | Bio Basic | 100ml | EUR 61.01 |
![]() Beaucage reagent |
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HY-100951 | MedChemExpress | 10mM/1mL | EUR 126 |
![]() BOP reagent |
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5-02141 | CHI Scientific | 25g | Ask for price |
![]() BOP reagent |
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5-02142 | CHI Scientific | 100g | Ask for price |
![]() Bluing Reagent |
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BRT030 | ScyTek Laboratories | 30 ml | EUR 60 |
![]() Bluing Reagent |
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BRT125 | ScyTek Laboratories | 125 ml | EUR 63 |
![]() Bluing Reagent |
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BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 184 |
![]() Bluing Reagent |
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BRT500 | ScyTek Laboratories | 500 ml | EUR 76 |
![]() Bluing Reagent |
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BRT999 | ScyTek Laboratories | 1000 ml | EUR 88 |
![]() BOP reagent |
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A7015-100000 | ApexBio | 100 g | EUR 200 |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
![]() BOP reagent |
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A7015-25000 | ApexBio | 25 g | EUR 113 |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
![]() Traut's Reagent |
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2330-1000 | Biovision | EUR 349 |
![]() Traut's Reagent |
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2330-500 | Biovision | EUR 207 |
![]() MTS Reagent |
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2808-1000 | Biovision | EUR 990 |
![]() MTS Reagent |
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2808-250 | Biovision | EUR 365 |
![]() MTT Reagent |
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2809-1G | Biovision | EUR 180 |
![]() MTT Reagent |
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2809-5G | Biovision | EUR 544 |
![]() Chymase reagent |
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30C-CP1129 | Fitzgerald | 5 units | EUR 2185 |
Description: Purified native Human Chymase reagent |
![]() GSK3A (pS21) Antibody |
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20-abx123381 | Abbexa |
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![]() GSK3A Rabbit pAb |
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A0645-100ul | Abclonal | 100 ul | EUR 308 |
![]() GSK3A Rabbit pAb |
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A0645-200ul | Abclonal | 200 ul | EUR 459 |
![]() GSK3A Rabbit pAb |
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A0645-20ul | Abclonal | 20 ul | EUR 183 |
![]() GSK3A Rabbit pAb |
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A0645-50ul | Abclonal | 50 ul | EUR 223 |
![]() GSK3A (pS21) Antibody |
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20-abx000172 | Abbexa |
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![]() Anti-GSK3A antibody |
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STJ23877 | St John's Laboratory | 100 µl | EUR 277 |
Description: This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. |
![]() GSK3A / GSK3B Antibody |
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20-abx328487 | Abbexa |
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![]() anti- GSK3A antibody |
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FNab03672 | FN Test | 100µg | EUR 505.25 |
Description: Antibody raised against GSK3A |
![]() GSK3A/GSK3B Antibody |
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1-CSB-PA002838 | Cusabio |
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Description: A polyclonal antibody against GSK3A/GSK3B. Recognizes GSK3A/GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
![]() anti- GSK3A antibody |
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FNab10243 | FN Test | 100µg | EUR 505.25 |
Description: Antibody raised against GSK3A |
![]() GSK3A Conjugated Antibody |
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C36917 | SAB | 100ul | EUR 397 |
![]() GSK3A Conjugated Antibody |
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C43681 | SAB | 100ul | EUR 397 |
![]() GSK3A/B Antibody |
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DF6806 | Affbiotech | 200ul | EUR 304 |
Description: GSK3A/B Antibody detects endogenous levels of total GSK3A/B. |
![]() Anti-GSK3A antibody |
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PAab03672 | Lifescience Market | 100 ug | EUR 355 |
![]() GSK3A Blocking Peptide |
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33R-6426 | Fitzgerald | 100 ug | EUR 180 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of GSK3A antibody, catalog no. 20R-1287 |
![]() LP4K Transfection Reagent |
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LP4K | GenTarget | 1.0 ml / vial | EUR 304 |
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types. |
![]() Tri-RNA Reagent |
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FATRR-001 | Favorgen | 100ml | EUR 236 |
![]() Tri-RNA Reagent |
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FATRR-002 | Favorgen | 50ml | EUR 176 |
![]() Tri-RNA Reagent |
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FATRR-003 | Favorgen | 450ml | EUR 645 |
![]() DTT (Cleland's reagent) |
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DB0058 | Bio Basic | 5g | EUR 84.8 |
![]() DTNB (Ellman's Reagent) |
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DB0113 | Bio Basic | 5g | EUR 97.85 |
![]() n-Heptane Reagent |
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HC5400 | Bio Basic | 1L | EUR 79 |
![]() PhosphoBlocker Blocking Reagent |
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AKR-104 | Cell Biolabs | 4L | EUR 711 |
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
![]() PhosphoBlocker Blocking Reagent |
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AKR-103 | Cell Biolabs | 1L | EUR 328 |
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
![]() Ethyl acetate Reagent |
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EC4600 | Bio Basic | 1L | EUR 79 |
![]() EL Transfection Reagent |
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20-abx098880 | Abbexa |
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![]() Mycoplasma Prevention Reagent |
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20-abx098886 | Abbexa |
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![]() Convoy? Transfection Reagent |
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1110-1ml | ACTGene | EUR 341 |
![]() FcR blocking Reagent |
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20-abx290024 | Abbexa |
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![]() Detection Reagent A |
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abx296004-120ul | Abbexa | 120 ul | EUR 321 |
![]() Mycoplasma Prevention Reagent |
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20-abx298005 | Abbexa |
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![]() Girard's reagent T |
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20-abx184099 | Abbexa |
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![]() TissueDigest Reagent, 20X |
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T101 | Insitus Biotechnologies | 10ml | EUR 210 |
![]() HighGene transfection reagent |
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RM09014 | Abclonal | 1000μl | EUR 270 |
![]() Griess Reagent Kit |
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30100 | Biotium | 1KIT | EUR 149 |
Description: Minimum order quantity: 1 unit of 1KIT |
![]() Biolipidure-1002-Reagent |
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Biolipidure-1002-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-103-Reagent |
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Biolipidure-103-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-103-Reagent |
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Biolipidure-103-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-1201-Reagent |
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Biolipidure-1201-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-1201-Reagent |
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Biolipidure-1201-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-1301-Reagent |
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Biolipidure-1301-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-1301-Reagent |
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Biolipidure-1301-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-203-Reagent |
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Biolipidure-203-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-203-Reagent |
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Biolipidure-203-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-206-Reagent |
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Biolipidure-206-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-206-Reagent |
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Biolipidure-206-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-405-Reagent |
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Biolipidure-405-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-405-Reagent |
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Biolipidure-405-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-502-Reagent |
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Biolipidure-502-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-502-Reagent |
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Biolipidure-502-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-702-Reagent |
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Biolipidure-702-10 | Biolipidure | 10mL | EUR 196 |
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
![]() Biolipidure-702-Reagent |
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Biolipidure-702-100 | Biolipidure | 100mL | EUR 1223 |
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
The AKT activator SC79 prompts GSK3 β/β-catenin signaling whereas the Wnt inhibitor XAV-939 abolishes 3-HAA inhibition of HCC progress in vitro and in mice. The present examine demonstrates that 3-HAA sensitizes HCC cells to sorafenib by lowering tumor stemness, suggesting it’s a promising molecule for HCC remedy.
Tags: 293t bl21 de3 star e2 crimson jm110 kpni restriction site lenticrispr mcherry plasmid paav mcs pacgfp1 n1 pacyc pbi121 pcag gfp pcdh vector pcdna3 ha pcdna5 frt pcep4 pci neo pci vector pcr blunt pcrii pcs2 vector pdonr201 pegfp c1 vector pegfp plasmid pet 15b pet15b sequence pet28 pet28a sequence pet28c pet32a pet41a petduet 1 pgbkt7 pgex 6p 2 pgex plasmid pgipz pgl3 promoter pgl3 sequence plko puro plvx tight puro pmcherry pmscv puro pqe80l prs315 prset a psti restriction enzyme ptripz puc 19 puc18 sequence puc19 plasmid tet3g uas sequence