Hippeastrum hybrid Lectin (HHL)
Product name
Hybrid Hippeastrum Lectin
Full Product Name
Hybrid Hippeastrum Lectin (HHL, Lectin Amaryllis, AL) (Biotin)
Purity / Purification
Degree in Molecular Biology
Shape / Format
Supplied as a powder in 10 mM HEPES, pH 7.5, 0.15 M sodium chloride, 0.08% sodium azide. Reconstitute with 1 ml of dH2O. Labeled with biotin.
Preparation and storage
The lyophilized powder can be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. The reconstituted product is stable for 6 months at -20 degrees C. For maximum product recovery, centrifuge the original vial after thawing and before removing the cap. Additional dilutions can be made in assay buffer.
Other notes
Occasionally, small volumes of Hippeastrum Hybrid Lectin vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Related Product Information for Lectin Hybrid Lectin Hippeastrum
This 50 kD tetrameric lectin is composed of four subunits of identical size and has an acidic isoelectric point. This lectin does not contain attached carbohydrates and is immunologically related to LNG and NPL. Amaryllis lectin binds only alpha mannose residues, not alpha glucosyl structures in contrast to Con A, PSA, and LCA. Like NPL, there appears to be an extended binding site for polymannose structures, which does not require mannose to be at the non-reducing end.
HHL binds to attached mannose structures (a -1.3) and (a-1,6), as well as some yeast galactomannans. This biotinylated lectin conjugate is prepared from affinity-purified lectin and optimally labeled with biotin. Essentially free of inactive lectin conjugate and no free biotin, this biotinylated lectin provides an ideal intermediate for screening glycoconjugates using the biotin-avidin system. Biotin-labeled lectin is added first, followed by an ABC reagent, an avidin D conjugate, or a streptavidin derivative. Another possible application is the isolation of lymphokines and other mitogenic stimulation products.
Precautions
All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.
Disclaimer
While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
