A framework for highly multiplexed dextramer mapping
A framework for extremely multiplexed dextramer mapping and prediction of T cell receptor sequences to antigen specificity
T cell receptor (TCR) antigen-specific recognition is important for the adaptive immune system. Nonetheless, constructing a TCR-antigen interplay map has been difficult because of the staggering range of TCRs and antigens. Accordingly, extremely multiplexed dextramer-TCR binding assays have been lately developed, however the utility of the following giant datasets is proscribed by the dearth of strong computational strategies for normalization and interpretation.
Right here, we current a computational framework comprising a novel technique, ICON (Integrative COntext-specific Normalization), for figuring out dependable TCR-pMHC (peptide-major histocompatibility complicated) interactions and a neural network-based classifier TCRAI that outperforms different state-of-the-art strategies for TCR-antigen specificity prediction. We additional demonstrated that by combining ICON and TCRAI, we’re in a position to uncover novel subgroups of TCRs that bind to a given pMHC through completely different mechanisms. Our framework facilitates the identification and understanding of TCR-antigen-specific interactions for fundamental immunological analysis and scientific immune monitoring.
Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-200ug |
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| QP5959-ec-200ug | EnQuireBio | 200ug | EUR 760.8 |
Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-500ug |
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| QP5959-ec-500ug | EnQuireBio | 500ug | EUR 1272 |
EBAG9 protein (His tag) |
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| 80R-1093 | Fitzgerald | 100 ug | EUR 268.8 |
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Description: Purified recombinant Human EBAG9 protein |
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Recombinant CD155 Protein (Asp 28-Asn 343) |
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| VAng-1181Lsx-100g | Creative Biolabs | 100 µg | EUR 1215.6 |
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Description: Rhesus macaque CD155 is expressed in HEK 293 cells. (Uniprot ID: Q0MSE6-1) |
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Recombinant CD155 Protein (Asp 28-Asn 343) |
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| VAng-1181Lsx-1mg | Creative Biolabs | 1 mg | EUR 7682.4 |
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Description: Rhesus macaque CD155 is expressed in HEK 293 cells. (Uniprot ID: Q0MSE6-1) |
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Recombinant ALCAM Protein (Trp 28-Ala 526) [Fc] |
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| VAng-0714Lsx-1mg | Creative Biolabs | 1 mg | EUR 3294 |
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Description: Human ALCAM, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_001618) |
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Recombinant ALCAM Protein (Trp 28-Ala 526) [Fc] |
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| VAng-0714Lsx-200g | Creative Biolabs | 200 µg | EUR 951.6 |
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Description: Human ALCAM, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_001618) |
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8430410A17Rik Recombinant Protein (Mouse) |
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| RP112721 | ABM | 100 ug | Ask for price |
9030625A04Rik Recombinant Protein (Mouse) |
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| RP112763 | ABM | 100 ug | Ask for price |
9430007A20Rik Recombinant Protein (Mouse) |
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| RP112820 | ABM | 100 ug | Ask for price |
1110001A16Rik Recombinant Protein (Mouse) |
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| RP109673 | ABM | 100 ug | Ask for price |
1110001A16Rik Recombinant Protein (Mouse) |
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| RP109676 | ABM | 100 ug | Ask for price |
1110005A03Rik Recombinant Protein (Mouse) |
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| RP109697 | ABM | 100 ug | Ask for price |
1110032A03Rik Recombinant Protein (Mouse) |
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| RP109781 | ABM | 100 ug | Ask for price |
1110032A04Rik Recombinant Protein (Mouse) |
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| RP109784 | ABM | 100 ug | Ask for price |
1110032A04Rik Recombinant Protein (Mouse) |
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| RP109787 | ABM | 100 ug | Ask for price |
1110034A24Rik Recombinant Protein (Mouse) |
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| RP109793 | ABM | 100 ug | Ask for price |
1520402A15Rik Recombinant Protein (Mouse) |
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| RP109955 | ABM | 100 ug | Ask for price |
1700006A11Rik Recombinant Protein (Mouse) |
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| RP110042 | ABM | 100 ug | Ask for price |
1700008A04Rik Recombinant Protein (Mouse) |
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| RP110066 | ABM | 100 ug | Ask for price |
1700011A15Rik Recombinant Protein (Mouse) |
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| RP110105 | ABM | 100 ug | Ask for price |
1700012A03Rik Recombinant Protein (Mouse) |
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| RP110129 | ABM | 100 ug | Ask for price |
1700012A16Rik Recombinant Protein (Mouse) |
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| RP110132 | ABM | 100 ug | Ask for price |
1700019A02Rik Recombinant Protein (Mouse) |
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| RP110213 | ABM | 100 ug | Ask for price |
1700020A23Rik Recombinant Protein (Mouse) |
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| RP110249 | ABM | 100 ug | Ask for price |
1700020A23Rik Recombinant Protein (Mouse) |
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| RP110252 | ABM | 100 ug | Ask for price |
1700023A16Rik Recombinant Protein (Mouse) |
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| RP110291 | ABM | 100 ug | Ask for price |
1700027A23Rik Recombinant Protein (Mouse) |
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| RP110336 | ABM | 100 ug | Ask for price |
1700027A23Rik Recombinant Protein (Mouse) |
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| RP110339 | ABM | 100 ug | Ask for price |
1810008A18Rik Recombinant Protein (Mouse) |
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| RP110603 | ABM | 100 ug | Ask for price |
1810009A15Rik Recombinant Protein (Mouse) |
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| RP110606 | ABM | 100 ug | Ask for price |
2010305A19Rik Recombinant Protein (Mouse) |
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| RP110798 | ABM | 100 ug | Ask for price |
2300009A05Rik Recombinant Protein (Mouse) |
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| RP110906 | ABM | 100 ug | Ask for price |
2310001A20Rik Recombinant Protein (Mouse) |
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| RP110909 | ABM | 100 ug | Ask for price |
2310022A10Rik Recombinant Protein (Mouse) |
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| RP110996 | ABM | 100 ug | Ask for price |
2310022A10Rik Recombinant Protein (Mouse) |
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| RP110999 | ABM | 100 ug | Ask for price |
2310046A06Rik Recombinant Protein (Mouse) |
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| RP111062 | ABM | 100 ug | Ask for price |
2410004A20Rik Recombinant Protein (Mouse) |
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| RP111134 | ABM | 100 ug | Ask for price |
2610528A11Rik Recombinant Protein (Mouse) |
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| RP111308 | ABM | 100 ug | Ask for price |
2810403A07Rik Recombinant Protein (Mouse) |
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| RP111407 | ABM | 100 ug | Ask for price |
2810408A11Rik Recombinant Protein (Mouse) |
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| RP111416 | ABM | 100 ug | Ask for price |
2900026A02Rik Recombinant Protein (Mouse) |
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| RP111464 | ABM | 100 ug | Ask for price |
2900053A13Rik Recombinant Protein (Mouse) |
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| RP111467 | ABM | 100 ug | Ask for price |
2900064A13Rik Recombinant Protein (Mouse) |
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| RP111470 | ABM | 100 ug | Ask for price |
3110003A17Rik Recombinant Protein (Mouse) |
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| RP111491 | ABM | 100 ug | Ask for price |
3830417A13Rik Recombinant Protein (Mouse) |
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| RP111569 | ABM | 100 ug | Ask for price |
4921523A10Rik Recombinant Protein (Mouse) |
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| RP111704 | ABM | 100 ug | Ask for price |
4930404A10Rik Recombinant Protein (Mouse) |
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| RP111758 | ABM | 100 ug | Ask for price |
4930427A07Rik Recombinant Protein (Mouse) |
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| RP111794 | ABM | 100 ug | Ask for price |
4930430A15Rik Recombinant Protein (Mouse) |
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| RP111803 | ABM | 100 ug | Ask for price |
4930431A04Rik Recombinant Protein (Mouse) |
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| RP111812 | ABM | 100 ug | Ask for price |
4930444A02Rik Recombinant Protein (Mouse) |
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| RP111839 | ABM | 100 ug | Ask for price |
4930465A12Rik Recombinant Protein (Mouse) |
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| RP111884 | ABM | 100 ug | Ask for price |
4930468A15Rik Recombinant Protein (Mouse) |
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| RP111890 | ABM | 100 ug | Ask for price |
4930505A04Rik Recombinant Protein (Mouse) |
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| RP111926 | ABM | 100 ug | Ask for price |
4930521A18Rik Recombinant Protein (Mouse) |
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| RP111953 | ABM | 100 ug | Ask for price |
4930557A04Rik Recombinant Protein (Mouse) |
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| RP112037 | ABM | 100 ug | Ask for price |
4931408A02Rik Recombinant Protein (Mouse) |
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| RP112133 | ABM | 100 ug | Ask for price |
4931408A02Rik Recombinant Protein (Mouse) |
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| RP112136 | ABM | 100 ug | Ask for price |
4933417A18Rik Recombinant Protein (Mouse) |
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| RP112316 | ABM | 100 ug | Ask for price |
6030405A18Rik Recombinant Protein (Mouse) |
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| RP112559 | ABM | 100 ug | Ask for price |
6330403A02Rik Recombinant Protein (Mouse) |
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| RP112592 | ABM | 100 ug | Ask for price |
6330545A04Rik Recombinant Protein (Mouse) |
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| RP112628 | ABM | 100 ug | Ask for price |
A130010J15Rik Recombinant Protein (Mouse) |
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| RP112922 | ABM | 100 ug | Ask for price |
A130010J15Rik Recombinant Protein (Mouse) |
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| RP112925 | ABM | 100 ug | Ask for price |
A130010J15Rik Recombinant Protein (Mouse) |
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| RP112928 | ABM | 100 ug | Ask for price |
A130022J15Rik Recombinant Protein (Mouse) |
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| RP112931 | ABM | 100 ug | Ask for price |
A230050P20Rik Recombinant Protein (Mouse) |
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| RP112943 | ABM | 100 ug | Ask for price |
A230051G13Rik Recombinant Protein (Mouse) |
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| RP112946 | ABM | 100 ug | Ask for price |
A230065H16Rik Recombinant Protein (Mouse) |
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| RP112949 | ABM | 100 ug | Ask for price |
A330049M08Rik Recombinant Protein (Mouse) |
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| RP112961 | ABM | 100 ug | Ask for price |
A330049M08Rik Recombinant Protein (Mouse) |
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| RP112964 | ABM | 100 ug | Ask for price |
A330049M08Rik Recombinant Protein (Mouse) |
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| RP112967 | ABM | 100 ug | Ask for price |
A330049M08Rik Recombinant Protein (Mouse) |
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| RP112970 | ABM | 100 ug | Ask for price |
A330049M08Rik Recombinant Protein (Mouse) |
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| RP112973 | ABM | 100 ug | Ask for price |
A330049M08Rik Recombinant Protein (Mouse) |
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| RP112976 | ABM | 100 ug | Ask for price |
A330050F15Rik Recombinant Protein (Mouse) |
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| RP112979 | ABM | 100 ug | Ask for price |
A430005L14Rik Recombinant Protein (Mouse) |
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| RP112988 | ABM | 100 ug | Ask for price |
A430005L14Rik Recombinant Protein (Mouse) |
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| RP112991 | ABM | 100 ug | Ask for price |
A430033K04Rik Recombinant Protein (Mouse) |
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| RP112994 | ABM | 100 ug | Ask for price |
A430078G23Rik Recombinant Protein (Mouse) |
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| RP112997 | ABM | 100 ug | Ask for price |
A430084P05Rik Recombinant Protein (Mouse) |
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| RP113000 | ABM | 100 ug | Ask for price |
A430089I19Rik Recombinant Protein (Mouse) |
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| RP113003 | ABM | 100 ug | Ask for price |
A430105I19Rik Recombinant Protein (Mouse) |
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| RP113006 | ABM | 100 ug | Ask for price |
A430107P09Rik Recombinant Protein (Mouse) |
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| RP113012 | ABM | 100 ug | Ask for price |
A530016L24Rik Recombinant Protein (Mouse) |
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| RP113027 | ABM | 100 ug | Ask for price |
A530032D15Rik Recombinant Protein (Mouse) |
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| RP113030 | ABM | 100 ug | Ask for price |
A530064D06Rik Recombinant Protein (Mouse) |
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| RP113036 | ABM | 100 ug | Ask for price |
A530064D06Rik Recombinant Protein (Mouse) |
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| RP113039 | ABM | 100 ug | Ask for price |
A530098C11Rik Recombinant Protein (Mouse) |
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| RP113042 | ABM | 100 ug | Ask for price |
A530099J19Rik Recombinant Protein (Mouse) |
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| RP113045 | ABM | 100 ug | Ask for price |
A630001G21Rik Recombinant Protein (Mouse) |
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| RP113048 | ABM | 100 ug | Ask for price |
A630033H20Rik Recombinant Protein (Mouse) |
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| RP113057 | ABM | 100 ug | Ask for price |
A630033H20Rik Recombinant Protein (Mouse) |
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| RP113060 | ABM | 100 ug | Ask for price |
A630033H20Rik Recombinant Protein (Mouse) |
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| RP113063 | ABM | 100 ug | Ask for price |
A630055G03Rik Recombinant Protein (Mouse) |
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| RP113066 | ABM | 100 ug | Ask for price |
A630073D07Rik Recombinant Protein (Mouse) |
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| RP113069 | ABM | 100 ug | Ask for price |
A630095E13Rik Recombinant Protein (Mouse) |
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| RP113075 | ABM | 100 ug | Ask for price |
A630095N17Rik Recombinant Protein (Mouse) |
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| RP113078 | ABM | 100 ug | Ask for price |
A630095N17Rik Recombinant Protein (Mouse) |
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| RP113081 | ABM | 100 ug | Ask for price |
A730008H23Rik Recombinant Protein (Mouse) |
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| RP113084 | ABM | 100 ug | Ask for price |
A730011L01Rik Recombinant Protein (Mouse) |
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| RP113087 | ABM | 100 ug | Ask for price |
A730011L01Rik Recombinant Protein (Mouse) |
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| RP113090 | ABM | 100 ug | Ask for price |
A730017C20Rik Recombinant Protein (Mouse) |
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| RP113093 | ABM | 100 ug | Ask for price |
A730017C20Rik Recombinant Protein (Mouse) |
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| RP113096 | ABM | 100 ug | Ask for price |
A730037C10Rik Recombinant Protein (Mouse) |
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| RP113099 | ABM | 100 ug | Ask for price |
A830007P12Rik Recombinant Protein (Mouse) |
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| RP113102 | ABM | 100 ug | Ask for price |
A830018L16Rik Recombinant Protein (Mouse) |
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| RP113111 | ABM | 100 ug | Ask for price |
A830018L16Rik Recombinant Protein (Mouse) |
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| RP113114 | ABM | 100 ug | Ask for price |
A830018L16Rik Recombinant Protein (Mouse) |
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| RP113117 | ABM | 100 ug | Ask for price |
A830018L16Rik Recombinant Protein (Mouse) |
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| RP113120 | ABM | 100 ug | Ask for price |
A830080D01Rik Recombinant Protein (Mouse) |
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| RP113123 | ABM | 100 ug | Ask for price |
A830093I24Rik Recombinant Protein (Mouse) |
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| RP113126 | ABM | 100 ug | Ask for price |
A930001N09Rik Recombinant Protein (Mouse) |
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| RP113129 | ABM | 100 ug | Ask for price |
A930005I04Rik Recombinant Protein (Mouse) |
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| RP113132 | ABM | 100 ug | Ask for price |
A930018P22Rik Recombinant Protein (Mouse) |
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| RP113138 | ABM | 100 ug | Ask for price |
A930038C07Rik Recombinant Protein (Mouse) |
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| RP113141 | ABM | 100 ug | Ask for price |
Recombinant ALCAM Protein (Trp 28-Ala 526) [His] |
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| VAng-0715Lsx-1mg | Creative Biolabs | 1 mg | EUR 3096 |
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Description: Human ALCAM, His tag, is expressed in HEK 293 cells. (Uniprot ID: Q13740-1) |
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Recombinant ALCAM Protein (Trp 28-Ala 526) [His] |
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| VAng-0715Lsx-200g | Creative Biolabs | 200 µg | EUR 885.6 |
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Description: Human ALCAM, His tag, is expressed in HEK 293 cells. (Uniprot ID: Q13740-1) |
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Recombinant Erythropoietin Protein (Ala 28-Arg 193) |
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| VAng-1516Lsx-20g | Creative Biolabs | 20 µg | EUR 712.8 |
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Description: Human Erythropoietin (EPO) is expressed in HEK 293 cells. (Uniprot ID: AAH93628.1) |
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Recombinant Erythropoietin Protein (Ala 28-Arg 193) |
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| VAng-1516Lsx-50g | Creative Biolabs | 50 µg | EUR 1215.6 |
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Description: Human Erythropoietin (EPO) is expressed in HEK 293 cells. (Uniprot ID: AAH93628.1) |
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S100 calcium binding protein A15A Recombinant Protein |
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| 92-713 | ProSci | 0.05 mg | EUR 588.3 |
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Description: S100 calcium binding protein A15A Recombinant Protein |
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EBAG9 Protein Vector (Rat) (pPM-C-HA) |
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| PV265296 | ABM | 500 ng | EUR 723.6 |
EBAG9 Protein Vector (Rat) (pPB-C-His) |
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| PV265294 | ABM | 500 ng | EUR 723.6 |
EBAG9 Protein Vector (Rat) (pPB-N-His) |
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| PV265295 | ABM | 500 ng | EUR 723.6 |
EBAG9 Protein Vector (Rat) (pPM-C-His) |
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| PV265297 | ABM | 500 ng | EUR 723.6 |
EBAG9 Protein Vector (Human) (pPM-C-HA) |
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| PV013463 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Human) (pPM-C-HA) |
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| PV013467 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Mouse) (pPM-C-HA) |
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| PV174240 | ABM | 500 ng | EUR 723.6 |
RNU6-28 Recombinant Protein (Human) |
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| RP089073 | ABM | 100 ug | Ask for price |
EBAG9 Protein Vector (Human) (pPB-C-His) |
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| PV013461 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Human) (pPB-N-His) |
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| PV013462 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Human) (pPM-C-His) |
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| PV013464 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Human) (pPB-C-His) |
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| PV013465 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Human) (pPB-N-His) |
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| PV013466 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Human) (pPM-C-His) |
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| PV013468 | ABM | 500 ng | EUR 394.8 |
EBAG9 Protein Vector (Mouse) (pPB-C-His) |
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| PV174238 | ABM | 500 ng | EUR 723.6 |
EBAG9 Protein Vector (Mouse) (pPB-N-His) |
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| PV174239 | ABM | 500 ng | EUR 723.6 |
EBAG9 Protein Vector (Mouse) (pPM-C-His) |
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| PV174241 | ABM | 500 ng | EUR 723.6 |
Recombinant Brucella Ovis BOV_A0350 Protein (aa 1-302) |
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| VAng-Lsx5507-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Brucella Ovis (strain ATCC 25840 / 63/290 / NCTC 10512) Putative peptide permease protein BOV_A0350, recombinant protein. |
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Recombinant Brucella Ovis BOV_A0688 Protein (aa 27-147) |
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| VAng-Lsx5512-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Brucella Ovis (strain ATCC 25840 / 63/290 / NCTC 10512) Lectin-like protein BA14k, recombinant protein. |
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Recombinant HPV-28 E1 Protein |
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| VAng-Wyb3131-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Human papillomavirus type 28 Replication protein E1, recombinant protein. |
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Recombinant HPV-28 L1 Protein |
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| VAng-Wyb3136-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Human papillomavirus type 28 Major capsid protein L1, recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0006 Protein (aa 1-239) |
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| VAng-Cr3263-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4468.8 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) probable transcriptional regulatory protein VC_A0006 (VC_A0006), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0006 Protein (aa 1-239) |
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| VAng-Cr3263-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3190.8 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) probable transcriptional regulatory protein VC_A0006 (VC_A0006), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0006 Protein (aa 1-239) |
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| VAng-Cr3263-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2166 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) probable transcriptional regulatory protein VC_A0006 (VC_A0006), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0042 Protein (aa 1-252) |
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| VAng-Cr3321-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4567.2 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) cyclic di-GMP binding protein VCA0042 (VC_A0042), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0042 Protein (aa 1-252) |
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| VAng-Cr3321-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3261.6 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) cyclic di-GMP binding protein VCA0042 (VC_A0042), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0042 Protein (aa 1-252) |
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| VAng-Cr3321-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2222.4 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) cyclic di-GMP binding protein VCA0042 (VC_A0042), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A1059 Protein (aa 1-201) |
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| VAng-Cr3389-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4188 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) putative pseudouridine methyltransferase (VC_A1059), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A1059 Protein (aa 1-201) |
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| VAng-Cr3389-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2980.8 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) putative pseudouridine methyltransferase (VC_A1059), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A1059 Protein (aa 1-201) |
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| VAng-Cr3389-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) putative pseudouridine methyltransferase (VC_A1059), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0354 Protein (aa 1-188) |
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| VAng-Cr2814-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4075.2 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0157 protein VC_A0354 (VC_A0354), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0354 Protein (aa 1-188) |
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| VAng-Cr2814-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2910 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0157 protein VC_A0354 (VC_A0354), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0354 Protein (aa 1-188) |
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| VAng-Cr2814-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1983.6 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0157 protein VC_A0354 (VC_A0354), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0026 Protein (aa 22-211) |
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| VAng-Cr2874-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4075.2 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0319 protein VC_A0026 (VC_A0026), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0026 Protein (aa 22-211) |
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| VAng-Cr2874-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2910 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0319 protein VC_A0026 (VC_A0026), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0026 Protein (aa 22-211) |
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| VAng-Cr2874-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1983.6 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0319 protein VC_A0026 (VC_A0026), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0741 Protein (aa 1-106) |
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| VAng-Cr2908-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3486 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0265 protein VC_A0741 (VC_A0741), recombinant protein. |
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Recombinant Vibrio Cholerae VC_A0741 Protein (aa 1-106) |
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| VAng-Cr2908-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2503.2 |
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Description: Vibrio Cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) UPF0265 protein VC_A0741 (VC_A0741), recombinant protein. |
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Quantifying persistence within the T-cell signaling community utilizing an optically controllable antigen receptor
T cells discriminate between wholesome and contaminated cells with outstanding sensitivity when mounting an immune response, which is hypothesized to rely upon T cells combining stimuli from a number of antigen-presenting cell interactions right into a stronger response. To quantify the capability for T cells to perform this, now we have developed an antigen receptor that’s optically tunable inside cell conjugates, offering management over the period, and depth of intracellular T-cell signaling.
We observe restricted persistence throughout the T-cell intracellular community on disruption of receptor enter, with alerts dissipating completely in ~15 min, and straight present sustained proximal receptor signaling is required to keep up gene transcription. T cells thus primarily accumulate the outputs of gene expression slightly than combine discrete intracellular alerts. Engineering optical management in a clinically related chimeric antigen receptor (CAR), we present that this restricted sign persistence might be exploited to extend CAR-T cell activation threefold utilizing pulsatile stimulation. Our outcomes are prone to apply extra typically to the signaling dynamics of different mobile networks.
Tumor rejection properties of gp100 209-specific T cells correlate with T cell receptor binding affinity in the direction of the wild kind slightly than anchor-modified antigen
Though there are exceptions and outliers, T cell practical responses typically correlate with the affinity of a TCR for a peptide/MHC complicated. In a single lately described outlier case, probably the most promising scientific candidate in a collection of TCRs particular for the gp100209 melanoma antigen sure with the weakest answer affinity and produced the least quantity of cytokine in vitro. Hypotheses for this outlier habits included uncommon cytokine expression patterns arising from an atypical TCR binding geometry. Finding out this occasion in additional element, we discovered right here that outlier habits is attributable to not uncommon cytokine patterns or TCR binding, however using a place 2 anchor-modified peptide variant in in vitro experiments as a substitute of the wild kind antigen that’s current in vivo.
Though the anchor-modified variant has been extensively utilized in fundamental and scientific immunology as a surrogate for the wild kind peptide, prior work has proven that TCRs can clearly distinguish between the 2. We present that when this differential recognition is accounted for, the practical properties of gp100209-specific TCRs monitor with their affinity in the direction of the peptide/MHC complicated. Past demonstrating the correlates with T cell operate for a clinically related TCR, our outcomes present vital concerns for collection of TCRs for immunotherapy and using modified peptides in immunology.
Proliferating cell nuclear antigen straight interacts with androgen receptor and enhances androgen receptor‑mediated signaling
Androgen receptor (AR) and/or its constitutively lively splicing variants (AR‑Vs), resembling AR‑V7 and ARv567es, is required for prostate most cancers cell development and survival, and most cancers development. Proliferating cell nuclear antigen (PCNA) is preferentially overexpressed in all cancers and executes its features by interplay with quite a few accomplice proteins. The intention of the current research was to analyze the potential position of PCNA within the regulation of AR exercise.
An an identical consensus sequence of the PCNA‑interacting protein‑field (PIP‑field) was recognized on the N‑terminus of human, mouse and rat AR proteins. It was discovered that PCNA complexes with the total‑size AR (AR‑FL) and AR‑V7, which might be attenuated by the small molecule PIP‑field inhibitor, T2AA. PCNA additionally complexes with ARv567es and recombinant AR protein. The PCNA inhibitors, PCNA‑I1S and T2AA, inhibited AR transcriptional exercise and the expression of AR goal genes in LNCaP‑AI and 22Rv1 cells, however not in AR‑unfavorable PC‑three cells. The knockdown of PCNA expression lowered dihydrotestosterone‑stimulated AR transcriptional exercise and abolished the inhibitory impact of PCNA‑I1S on AR exercise. The PCNA inhibitor, PCNA‑I1, exerted additive development inhibitory results with androgen deprivation and enzalutamide in cells expressing AR‑FL or AR‑FL/AR‑V7, however not in AR‑unfavorable PC‑three cells.
Lastly, R9‑AR‑PIP, a small peptide mimicking AR PIP‑field, was discovered to bind to GFP‑PCNA at Okayd of two.73 µM and inhibit the expression of AR goal genes, AR transcriptional exercise and the expansion of AR‑expressing cells. On the entire, these information strongly counsel that AR is a PCNA accomplice protein and interacts with PCNA through the PIP‑field and that concentrating on the PCNA‑AR interplay could signify an revolutionary and selective therapeutic technique towards prostate most cancers, significantly castration‑resistant prostate cancers overexpressing constitutively lively AR‑Vs.
Novel high-affinity EGFRvIII-specific chimeric antigen receptor T cells successfully remove human glioblastoma
Aims: The growing success of Chimeric Antigen Receptor (CAR) T cell remedy in haematological malignancies is reinvigorating its utility in lots of different most cancers sorts and with renewed deal with its utility to stable tumors. We current a novel CAR towards glioblastoma, an aggressive, malignant glioma, with a dismal survival charge for which remedy choices have remained unchanged for over a decade.
Strategies: We use the human Retained Show (ReD) antibody platform (Myrio Therapeutics) to determine a novel single-chain variable fragment (scFv) that recognises epidermal development issue receptor mutant variant III (EGFRvIII), a typical and tumor-specific mutation present in glioblastoma. We use each in vitro practical assays and an in vivo orthotopic xenograft mannequin of glioblastoma to look at the operate of our novel CAR, known as GCT02, focused utilizing murine CAR T cells.
Outcomes: Our EGFRvIII-specific scFv was discovered to be of a lot increased affinity than reported comparators reverse-engineered from monoclonal antibodies. Regardless of the upper affinity, GCT02 CAR T cells kill equivalently however secrete decrease quantities of cytokine. As well as, GCT02-CAR T cells additionally mediate fast and full tumor elimination in vivo.
Conclusion: We current a novel EGFRvIII-specific CAR, with efficient antitumor features each in in vitro and in a xenograft mannequin of human glioblastoma.
XM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV756075 | ABM | 1.0 ug DNA | Ask for price |
XS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV756093 | ABM | 1.0 ug DNA | Ask for price |
HGF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV701983 | ABM | 1.0 ug DNA | EUR 540 |
C8A Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV702505 | ABM | 1.0 ug DNA | EUR 540 |
MIP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV706507 | ABM | 1.0 ug DNA | EUR 540 |
AEN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707161 | ABM | 1.0 ug DNA | EUR 379.2 |
AEN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707167 | ABM | 1.0 ug DNA | EUR 379.2 |
AGK Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707191 | ABM | 1.0 ug DNA | EUR 379.2 |
ALB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707275 | ABM | 1.0 ug DNA | EUR 379.2 |
ANG Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707329 | ABM | 1.0 ug DNA | EUR 379.2 |
ATM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707659 | ABM | 1.0 ug DNA | EUR 379.2 |
BID Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707851 | ABM | 1.0 ug DNA | EUR 379.2 |
BMF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707875 | ABM | 1.0 ug DNA | EUR 379.2 |
BMF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707881 | ABM | 1.0 ug DNA | EUR 379.2 |
CA8 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708259 | ABM | 1.0 ug DNA | EUR 379.2 |
CA8 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708265 | ABM | 1.0 ug DNA | EUR 379.2 |
CBS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708343 | ABM | 1.0 ug DNA | EUR 379.2 |
CBS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708349 | ABM | 1.0 ug DNA | EUR 379.2 |
CGA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708673 | ABM | 1.0 ug DNA | EUR 379.2 |
CKB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708757 | ABM | 1.0 ug DNA | EUR 379.2 |
CRK Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708991 | ABM | 1.0 ug DNA | EUR 379.2 |
CRP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV708997 | ABM | 1.0 ug DNA | EUR 379.2 |
CRP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV709003 | ABM | 1.0 ug DNA | EUR 379.2 |
DTL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV709471 | ABM | 1.0 ug DNA | EUR 379.2 |
LY9 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV705295 | ABM | 1.0 ug DNA | EUR 540 |
DUT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV705463 | ABM | 1.0 ug DNA | EUR 540 |
NPL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV705589 | ABM | 1.0 ug DNA | EUR 540 |
BMF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV705643 | ABM | 1.0 ug DNA | EUR 540 |
FTO Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV706003 | ABM | 1.0 ug DNA | EUR 540 |
UBB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV716533 | ABM | 1.0 ug DNA | EUR 379.2 |
CCT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720669 | ABM | 1.0 ug DNA | Ask for price |
CCV Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720723 | ABM | 1.0 ug DNA | Ask for price |
Sp2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV715321 | ABM | 1.0 ug DNA | EUR 379.2 |
Sp2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV715327 | ABM | 1.0 ug DNA | EUR 379.2 |
Syk Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV715609 | ABM | 1.0 ug DNA | EUR 379.2 |
SLN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV702973 | ABM | 1.0 ug DNA | EUR 540 |
CRP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV703483 | ABM | 1.0 ug DNA | EUR 540 |
NPL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV703699 | ABM | 1.0 ug DNA | EUR 540 |
LY9 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV703819 | ABM | 1.0 ug DNA | EUR 540 |
AIC Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV718395 | ABM | 1.0 ug DNA | Ask for price |
AOM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV718869 | ABM | 1.0 ug DNA | Ask for price |
ATD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV719175 | ABM | 1.0 ug DNA | Ask for price |
FGA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV704107 | ABM | 1.0 ug DNA | EUR 540 |
NNT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV704257 | ABM | 1.0 ug DNA | EUR 540 |
WAC Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV716731 | ABM | 1.0 ug DNA | EUR 379.2 |
AFA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV718317 | ABM | 1.0 ug DNA | Ask for price |
TK1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV715879 | ABM | 1.0 ug DNA | EUR 379.2 |
TTK Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV716389 | ABM | 1.0 ug DNA | EUR 379.2 |
ST2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV749655 | ABM | 1.0 ug DNA | EUR 686.4 |
ST3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV749661 | ABM | 1.0 ug DNA | Ask for price |
ST4 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV749673 | ABM | 1.0 ug DNA | Ask for price |
ST8 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV749691 | ABM | 1.0 ug DNA | Ask for price |
RP6 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV747189 | ABM | 1.0 ug DNA | Ask for price |
RSS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV747633 | ABM | 1.0 ug DNA | Ask for price |
S11 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV747687 | ABM | 1.0 ug DNA | Ask for price |
S12 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV747693 | ABM | 1.0 ug DNA | Ask for price |
SDU Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV748131 | ABM | 1.0 ug DNA | Ask for price |
SEA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV748137 | ABM | 1.0 ug DNA | Ask for price |
TDD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV750297 | ABM | 1.0 ug DNA | Ask for price |
THM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV750405 | ABM | 1.0 ug DNA | Ask for price |
TOC Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV750681 | ABM | 1.0 ug DNA | Ask for price |
PRS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV741753 | ABM | 1.0 ug DNA | Ask for price |
PRD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV741573 | ABM | 1.0 ug DNA | Ask for price |
FTX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV761757 | ABM | 1.0 ug DNA | EUR 1104 |
CPP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV759285 | ABM | 1.0 ug DNA | EUR 2148 |
CPQ Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV759291 | ABM | 1.0 ug DNA | EUR 686.4 |
UTX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV814423 | ABM | 1.0 ug DNA | EUR 540 |
PB1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV815389 | ABM | 1.0 ug DNA | EUR 540 |
PB1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811123 | ABM | 1.0 ug DNA | EUR 379.2 |
GBL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811201 | ABM | 1.0 ug DNA | EUR 379.2 |
NLP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811939 | ABM | 1.0 ug DNA | EUR 379.2 |
DCI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812305 | ABM | 1.0 ug DNA | EUR 379.2 |
RIP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812623 | ABM | 1.0 ug DNA | EUR 379.2 |
HNT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812743 | ABM | 1.0 ug DNA | EUR 379.2 |
DCI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV809323 | ABM | 1.0 ug DNA | EUR 379.2 |
GBL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV809377 | ABM | 1.0 ug DNA | EUR 379.2 |
ZAk Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV809521 | ABM | 1.0 ug DNA | EUR 379.2 |
GAS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV813451 | ABM | 1.0 ug DNA | EUR 379.2 |
GRN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV710665 | ABM | 1.0 ug DNA | EUR 379.2 |
HGF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV710833 | ABM | 1.0 ug DNA | EUR 379.2 |
HGF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV710839 | ABM | 1.0 ug DNA | EUR 379.2 |
HYI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV711145 | ABM | 1.0 ug DNA | EUR 379.2 |
JTB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV711559 | ABM | 1.0 ug DNA | EUR 379.2 |
LBH Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV711901 | ABM | 1.0 ug DNA | EUR 379.2 |
LUM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV712087 | ABM | 1.0 ug DNA | EUR 379.2 |
LXN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV712093 | ABM | 1.0 ug DNA | EUR 379.2 |
NF2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV712735 | ABM | 1.0 ug DNA | EUR 379.2 |
NMI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV712777 | ABM | 1.0 ug DNA | EUR 379.2 |
OAT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV712987 | ABM | 1.0 ug DNA | EUR 379.2 |
OS9 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV713023 | ABM | 1.0 ug DNA | EUR 379.2 |
