medmk

A framework for highly multiplexed dextramer mapping

A framework for extremely multiplexed dextramer mapping and prediction of T cell receptor sequences to antigen specificity

T cell receptor (TCR) antigen-specific recognition is important for the adaptive immune system. Nonetheless, constructing a TCR-antigen interplay map has been difficult because of the staggering range of TCRs and antigens. Accordingly, extremely multiplexed dextramer-TCR binding assays have been lately developed, however the utility of the following giant datasets is proscribed by the dearth of strong computational strategies for normalization and interpretation.

Right here, we current a computational framework comprising a novel technique, ICON (Integrative COntext-specific Normalization), for figuring out dependable TCR-pMHC (peptide-major histocompatibility complicated) interactions and a neural network-based classifier TCRAI that outperforms different state-of-the-art strategies for TCR-antigen specificity prediction. We additional demonstrated that by combining ICON and TCRAI, we’re in a position to uncover novel subgroups of TCRs that bind to a given pMHC through completely different mechanisms. Our framework facilitates the identification and understanding of TCR-antigen-specific interactions for fundamental immunological analysis and scientific immune monitoring.

medmk
medmk

EBAG9 (NM_004215) Human Recombinant Protein

PROTO00559 20ug
EUR 1249
Description: Recombinant protein of human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1

EBAG9 Protein, Human, Recombinant (His Tag)

MBS8121611-01mg 0.1mg
EUR 450

EBAG9 Protein, Human, Recombinant (His Tag)

MBS8121611-5x01mg 5x0.1mg
EUR 1885

OPCA03845-1MG - EBAG9 Recombinant Protein (Human)

OPCA03845-1MG 1mg
EUR 1379

OPCA03845-20UG - EBAG9 Recombinant Protein (Human)

OPCA03845-20UG 20ug
EUR 218

OPCA03845-100UG - EBAG9 Recombinant Protein (Human)

OPCA03845-100UG 100ug
EUR 323

Recombinant Human RCAS1 / EBAG9 protein (His tag)

PDEH100304-100ug 100ug
EUR 118
Description: Human

Recombinant Human RCAS1 / EBAG9 protein (His tag)

PDEH100304-20ug 20ug
EUR 68
Description: Human

Recombinant Human RCAS1/EBAG9 protein (His tag)

MBS2570902-002mg 0.02mg
EUR 135

Recombinant Human RCAS1/EBAG9 protein (His tag)

MBS2570902-01mg 0.1mg
EUR 170

Recombinant Human RCAS1/EBAG9 protein (His tag)

MBS2570902-5x01mg 5x0.1mg
EUR 765

Recombinant Rat EBAG9 Protein, His (Fc)-Avi-tagged

EBAG9-1654R 50ug
EUR 2398.4
Description: For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Recombinant Mouse EBAG9 Protein, His (Fc)-Avi-tagged

EBAG9-2613M 50ug
EUR 2398.4
Description: For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-1mg

QP5959-ec-1mg 1mg
EUR 1958.4

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-10ug

QP5959-ec-10ug 10ug
EUR 240

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-50ug

QP5959-ec-50ug 50ug
EUR 315.6

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-100ug

QP5959-ec-100ug 100ug
EUR 489.6

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-200ug

QP5959-ec-200ug 200ug
EUR 760.8

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-500ug

QP5959-ec-500ug 500ug
EUR 1272

EBAG9 protein (His tag)

80R-1093 100 ug
EUR 219
Description: Purified recombinant Human EBAG9 protein

Human EBAG9 Protein Lysate

MBS8410846-002mg 0.02mg
EUR 365

Human EBAG9 Protein Lysate

MBS8410846-5x002mg 5x0.02mg
EUR 1410

OPPA02054-10UG - EBAG9 Protein

OPPA02054-10UG 10ug
EUR 70

Human EBAG9 Protein, His Tag

E40KMPH4470 20ug
EUR 495

Human EBAG9 Protein Lysate 20ug

IHUEBAG9PLLY20UG each
EUR 213
Description: Human EBAG9 Protein Lysate 20ug

OCOA20218-20UG - EBAG9 Protein Lysate

OCOA20218-20UG 20ug
EUR 169

OCOA13214-20UG - EBAG9 Protein Lysate

OCOA13214-20UG 20ug
EUR 169

OCOA04155-20UG - EBAG9 Protein Lysate

OCOA04155-20UG 20ug
EUR 169

EBAG9 Protein Vector (Rat) (pPM-C-HA)

PV265296 500 ng
EUR 723.6

EBAG9 Protein Vector (Rat) (pPB-C-His)

PV265294 500 ng
EUR 723.6

EBAG9 Protein Vector (Rat) (pPB-N-His)

PV265295 500 ng
EUR 723.6

EBAG9 Protein Vector (Rat) (pPM-C-His)

PV265297 500 ng
EUR 723.6

EBAG9 Protein Vector (Human) (pPM-C-HA)

PV013463 500 ng
EUR 394.8

EBAG9 Protein Vector (Human) (pPM-C-HA)

PV013467 500 ng
EUR 394.8

EBAG9 Protein Vector (Mouse) (pPM-C-HA)

PV174240 500 ng
EUR 723.6

EBAG9 Protein Vector (Human) (pPB-C-His)

PV013461 500 ng
EUR 394.8

EBAG9 Protein Vector (Human) (pPB-N-His)

PV013462 500 ng
EUR 394.8

EBAG9 Protein Vector (Human) (pPM-C-His)

PV013464 500 ng
EUR 394.8

EBAG9 Protein Vector (Human) (pPB-C-His)

PV013465 500 ng
EUR 394.8

EBAG9 Protein Vector (Human) (pPB-N-His)

PV013466 500 ng
EUR 394.8

EBAG9 Protein Vector (Human) (pPM-C-His)

PV013468 500 ng
EUR 394.8

EBAG9 Protein Vector (Mouse) (pPB-C-His)

PV174238 500 ng
EUR 723.6

EBAG9 Protein Vector (Mouse) (pPB-N-His)

PV174239 500 ng
EUR 723.6

EBAG9 Protein Vector (Mouse) (pPM-C-His)

PV174241 500 ng
EUR 723.6

EBAG9

CSB-CL007355HU1 10 μg plasmid + 200μl Glycerol Ask for price

EBAG9

CSB-CL007355HU2 10 μg plasmid + 200μl Glycerol Ask for price

EBAG9

pro-343 10µg
EUR 60
Description: Recombinant Human Estrogen Receptor Binding Site Associated Antigen 9

Recombinant Dog Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

MBS1349708-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1115

Recombinant Dog Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

MBS1349708-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1335

Recombinant Dog Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

MBS1349708-05mgEColi 0.5mg(E-Coli)
EUR 1115

Recombinant Dog Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

MBS1349708-05mgYeast 0.5mg(Yeast)
EUR 1335

Recombinant Dog Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

MBS1349708-1mgEColi 1mg(E-Coli)
EUR 1635

Recombinant Rat Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1430359-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1115

Recombinant Rat Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1430359-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1335

Recombinant Rat Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1430359-05mgEColi 0.5mg(E-Coli)
EUR 1115

Recombinant Rat Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1430359-05mgYeast 0.5mg(Yeast)
EUR 1335

Recombinant Rat Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1430359-1mgEColi 1mg(E-Coli)
EUR 1635

Recombinant Human Receptor-binding cancer antigen expressed on SiSo cells(EBAG9),partial

AP70252 1mg
EUR 1978

Recombinant Human Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

CSB-EP007355HU 1056 mg Ask for price

Recombinant Mouse Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1320693-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1115

Recombinant Mouse Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1320693-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1335

Recombinant Mouse Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1320693-05mgEColi 0.5mg(E-Coli)
EUR 1115

Recombinant Mouse Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1320693-05mgYeast 0.5mg(Yeast)
EUR 1335

Recombinant Mouse Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

MBS1320693-1mgEColi 1mg(E-Coli)
EUR 1635

Monoclonal EBAG9 Antibody (monoclonal) (M04), Clone: 4A10

AMM03477G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human EBAG9 (monoclonal) (M04). The antibodies are raised in mouse and are from clone 4A10. This antibody is applicable in WB, E

RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9 Recombinant Rabbit Monoclonal Antibody [Clone EBAG9/7033R]

MBS4382356-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9 Recombinant Rabbit Monoclonal Antibody [Clone EBAG9/7033R]

MBS4382356-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9 Recombinant Rabbit Monoclonal Antibody [Clone EBAG9/7033R]

MBS4382356-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9 Recombinant Rabbit Monoclonal Antibody [Clone EBAG9/7033R]

MBS4382356-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9 Recombinant Rabbit Monoclonal Antibody [Clone EBAG9/7033R]

MBS4382356-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

EBAG9 siRNA

20-abx914879
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EBAG9 siRNA

20-abx914880
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EBAG9 siRNA

20-abx901624
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EBAG9 Peptide

45-515P 0.1 mg
EUR 405.6
Description: EBAG9 / RCAS1 Peptide

EBAG9 antibody

38323 100ul
EUR 439

EBAG9 antibody

38323-100ul 100ul
EUR 302.4

EBAG9 Antibody

DF6703 200ul
EUR 420

EBAG9 Antibody

DF6703-100ul 100ul
EUR 280

EBAG9 Antibody

DF6703-200ul 200ul
EUR 350

EBAG9 Antibody

E91935 100μg
EUR 255
Description: Available in various conjugation types.

EBAG9 Antibody

E300798 100ug/200ul
EUR 275
Description: Available in various conjugation types.

EBAG9 Antibody

E300799 100ug/200ul
EUR 275
Description: Available in various conjugation types.

EBAG9 antibody

70R-16981 50 ul
EUR 289
Description: Rabbit polyclonal EBAG9 antibody

EBAG9 antibody

70R-9916 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal EBAG9 antibody

EBAG9 Antibody

1-CSB-PA007355GA01HU
  • Ask for price
  • Ask for price
  • 150ul
  • 50ul
Description: A polyclonal antibody against EBAG9. Recognizes EBAG9 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

EBAG9 Antibody

ABD6703 100ug
EUR 325

EBAG9 Antibody

GWB-MV765I 50ug Ask for price

EBAG9 Antibody

R34393-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Estrogen receptor binding site associated, antigen, 9, RCAS1

EBAG9 Antibody

R31790 100 ug
EUR 356.15
Description: Receptor-binding cancer antigen expressed on SiSo cells is a protein that in humans is encoded by the EBAG9 gene. This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. And the encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.

EBAG9 Antibody

K40051M04H07C-100ug 100 ug
EUR 360
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M04H07C-1mg 1 mg
EUR 1800
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M04H07C-50ug 50 ug
EUR 252
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M10A09C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M10A09C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M10A09C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M14G10C-100ug 100 ug
EUR 280
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M14G10C-1mg 1 mg
EUR 1400
Description: Monoclonal Antibody

EBAG9 Antibody

K40051M14G10C-50ug 50 ug
EUR 196
Description: Monoclonal Antibody

EBAG9 antibody

MBS9405267-005mL 0.05mL
EUR 300

EBAG9 antibody

MBS9405267-01mL 0.1mL
EUR 390

EBAG9 antibody

MBS9405267-5x01mL 5x0.1mL
EUR 1610

EBAG9 Antibody

MBS854957-01mg 0.1mg
EUR 345

EBAG9 Antibody

MBS854957-01mLAF405L 0.1mL(AF405L)
EUR 565

EBAG9 Antibody

MBS854957-01mLAF405S 0.1mL(AF405S)
EUR 565

EBAG9 Antibody

MBS854957-01mLAF610 0.1mL(AF610)
EUR 565

EBAG9 Antibody

MBS854957-01mLAF635 0.1mL(AF635)
EUR 565

EBAG9 Antibody

MBS854258-01mg 0.1mg
EUR 345

EBAG9 Antibody

MBS854258-01mLAF405L 0.1mL(AF405L)
EUR 565

EBAG9 Antibody

MBS854258-01mLAF405S 0.1mL(AF405S)
EUR 565

EBAG9 Antibody

MBS854258-01mLAF610 0.1mL(AF610)
EUR 565

EBAG9 Antibody

MBS854258-01mLAF635 0.1mL(AF635)
EUR 565

EBAG9 Antibody

MBS8505315-01mg 0.1mg
EUR 325

EBAG9 Antibody

MBS8505315-01mLAF405L 0.1mL(AF405L)
EUR 565

EBAG9 Antibody

MBS8505315-01mLAF405S 0.1mL(AF405S)
EUR 565

EBAG9 Antibody

MBS8505315-01mLAF610 0.1mL(AF610)
EUR 565

EBAG9 Antibody

MBS8505315-01mLAF635 0.1mL(AF635)
EUR 565

EBAG9 Antibody

MBS2523105-006mL 0.06mL
EUR 190

EBAG9 Antibody

MBS2523105-012mL 0.12mL
EUR 265

EBAG9 Antibody

MBS2523105-02mL 0.2mL
EUR 415

EBAG9 Antibody

MBS2523105-5x02mL 5x0.2mL
EUR 1835

EBAG9 Antibody

MBS9607359-01mL 0.1mL
EUR 260

EBAG9 Antibody

MBS9607359-02mL 0.2mL
EUR 305

EBAG9 Antibody

MBS9607359-5x02mL 5x0.2mL
EUR 1220

Active Recombinant Human ICAM1 Protein (28-480aa), C-hIgG-His-tagged

ICAM1-14H 5ug
EUR 158.4
Description: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

EBAG9 siRNA (Rat)

MBS8206041-15nmol 15nmol
EUR 405

EBAG9 siRNA (Rat)

MBS8206041-30nmol 30nmol
EUR 565

EBAG9 siRNA (Rat)

MBS8206041-5x30nmol 5x30nmol
EUR 2450

EBAG9 Rabbit pAb

A8385-200ul 200 ul
EUR 550.8

EBAG9 Rabbit pAb

A8385-20ul 20 ul Ask for price

EBAG9 Rabbit pAb

E2501935 100ul
EUR 225
Description: Available in various conjugation types.

EBAG9 Rabbit pAb

A1935-100ul 100 ul
EUR 369.6

EBAG9 Rabbit pAb

A1935-200ul 200 ul
EUR 550.8

EBAG9 Rabbit pAb

A1935-20ul 20 ul
EUR 219.6

EBAG9 Rabbit pAb

A1935-50ul 50 ul
EUR 267.6

EBAG9 cDNA Clone

MBS1269322-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

EBAG9 cDNA Clone

MBS1269322-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

EBAG9 cDNA Clone

MBS1273084-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

EBAG9 cDNA Clone

MBS1273084-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

EBAG9 Rabbit pAb

MBS8544680-01mL 0.1mL
EUR 305

EBAG9 Rabbit pAb

MBS8544680-01mLAF405L 0.1mL(AF405L)
EUR 565

EBAG9 Rabbit pAb

MBS8544680-01mLAF405S 0.1mL(AF405S)
EUR 565

EBAG9 Rabbit pAb

MBS8544680-01mLAF610 0.1mL(AF610)
EUR 565

EBAG9 Rabbit pAb

MBS8544680-01mLAF635 0.1mL(AF635)
EUR 565

EBAG9 Rabbit pAb

A1935 50μL
EUR 173.58

EBAG9 Rabbit pAb

A8385 200μL
EUR 173.58

EBAG9 siRNA (Mouse)

MBS8232252-15nmol 15nmol
EUR 405

EBAG9 siRNA (Mouse)

MBS8232252-30nmol 30nmol
EUR 565

EBAG9 siRNA (Mouse)

MBS8232252-5x30nmol 5x30nmol
EUR 2450

EBAG9 siRNA (Human)

MBS8241103-15nmol 15nmol
EUR 405

EBAG9 siRNA (Human)

MBS8241103-30nmol 30nmol
EUR 565

EBAG9 siRNA (Human)

MBS8241103-5x30nmol 5x30nmol
EUR 2450

Human EBAG9 Antibody

32605-05111 150 ug
EUR 215

EBAG9 Antibody / RCAS1

V5262-100UG 100ug
EUR 363.3
Description: EBAG9 (Estrogen receptor-binding fragment-associated gene 9 protein), also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.

EBAG9 Antibody / RCAS1

V5262-20UG 20ug
EUR 160.3
Description: EBAG9 (Estrogen receptor-binding fragment-associated gene 9 protein), also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.

EBAG9 Antibody / RCAS1

V5262SAF-100UG 100ug
EUR 363.3
Description: EBAG9 (Estrogen receptor-binding fragment-associated gene 9 protein), also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.

EBAG9 cloning plasmid

CSB-CL007355HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the EBAG9 gene.

EBAG9 cloning plasmid

CSB-CL007355HU2-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the EBAG9 gene.

EBAG9 Antibody (Center)

MBS9232087-01mL 0.1mL
EUR 435

EBAG9 Antibody (Center)

MBS9232087-5x01mL 5x0.1mL
EUR 1625

EBAG9 Antibody (Center)

MBS9202634-008mL 0.08mL
EUR 210

Quantifying persistence within the T-cell signaling community utilizing an optically controllable antigen receptor

T cells discriminate between wholesome and contaminated cells with outstanding sensitivity when mounting an immune response, which is hypothesized to rely upon T cells combining stimuli from a number of antigen-presenting cell interactions right into a stronger response. To quantify the capability for T cells to perform this, now we have developed an antigen receptor that’s optically tunable inside cell conjugates, offering management over the period, and depth of intracellular T-cell signaling.

We observe restricted persistence throughout the T-cell intracellular community on disruption of receptor enter, with alerts dissipating completely in ~15 min, and straight present sustained proximal receptor signaling is required to keep up gene transcription. T cells thus primarily accumulate the outputs of gene expression slightly than combine discrete intracellular alerts. Engineering optical management in a clinically related chimeric antigen receptor (CAR), we present that this restricted sign persistence might be exploited to extend CAR-T cell activation threefold utilizing pulsatile stimulation. Our outcomes are prone to apply extra typically to the signaling dynamics of different mobile networks.

Tumor rejection properties of gp100 209-specific T cells correlate with T cell receptor binding affinity in the direction of the wild kind slightly than anchor-modified antigen

Though there are exceptions and outliers, T cell practical responses typically correlate with the affinity of a TCR for a peptide/MHC complicated. In a single lately described outlier case, probably the most promising scientific candidate in a collection of TCRs particular for the gp100209 melanoma antigen sure with the weakest answer affinity and produced the least quantity of cytokine in vitro. Hypotheses for this outlier habits included uncommon cytokine expression patterns arising from an atypical TCR binding geometry. Finding out this occasion in additional element, we discovered right here that outlier habits is attributable to not uncommon cytokine patterns or TCR binding, however using a place 2 anchor-modified peptide variant in in vitro experiments as a substitute of the wild kind antigen that’s current in vivo.

Though the anchor-modified variant has been extensively utilized in fundamental and scientific immunology as a surrogate for the wild kind peptide, prior work has proven that TCRs can clearly distinguish between the 2. We present that when this differential recognition is accounted for, the practical properties of gp100209-specific TCRs monitor with their affinity in the direction of the peptide/MHC complicated. Past demonstrating the correlates with T cell operate for a clinically related TCR, our outcomes present vital concerns for collection of TCRs for immunotherapy and using modified peptides in immunology.

Proliferating cell nuclear antigen straight interacts with androgen receptor and enhances androgen receptor‑mediated signaling

Androgen receptor (AR) and/or its constitutively lively splicing variants (AR‑Vs), resembling AR‑V7 and ARv567es, is required for prostate most cancers cell development and survival, and most cancers development. Proliferating cell nuclear antigen (PCNA) is preferentially overexpressed in all cancers and executes its features by interplay with quite a few accomplice proteins. The intention of the current research was to analyze the potential position of PCNA within the regulation of AR exercise.

An an identical consensus sequence of the PCNA‑interacting protein‑field (PIP‑field) was recognized on the N‑terminus of human, mouse and rat AR proteins. It was discovered that PCNA complexes with the total‑size AR (AR‑FL) and AR‑V7, which might be attenuated by the small molecule PIP‑field inhibitor, T2AA. PCNA additionally complexes with ARv567es and recombinant AR protein. The PCNA inhibitors, PCNA‑I1S and T2AA, inhibited AR transcriptional exercise and the expression of AR goal genes in LNCaP‑AI and 22Rv1 cells, however not in AR‑unfavorable PC‑three cells. The knockdown of PCNA expression lowered dihydrotestosterone‑stimulated AR transcriptional exercise and abolished the inhibitory impact of PCNA‑I1S on AR exercise. The PCNA inhibitor, PCNA‑I1, exerted additive development inhibitory results with androgen deprivation and enzalutamide in cells expressing AR‑FL or AR‑FL/AR‑V7, however not in AR‑unfavorable PC‑three cells.

Lastly, R9‑AR‑PIP, a small peptide mimicking AR PIP‑field, was discovered to bind to GFP‑PCNA at Okayd of two.73 µM and inhibit the expression of AR goal genes, AR transcriptional exercise and the expansion of AR‑expressing cells. On the entire, these information strongly counsel that AR is a PCNA accomplice protein and interacts with PCNA through the PIP‑field and that concentrating on the PCNA‑AR interplay could signify an revolutionary and selective therapeutic technique towards prostate most cancers, significantly castration‑resistant prostate cancers overexpressing constitutively lively AR‑Vs.

Novel high-affinity EGFRvIII-specific chimeric antigen receptor T cells successfully remove human glioblastoma

Aims: The growing success of Chimeric Antigen Receptor (CAR) T cell remedy in haematological malignancies is reinvigorating its utility in lots of different most cancers sorts and with renewed deal with its utility to stable tumors. We current a novel CAR towards glioblastoma, an aggressive, malignant glioma, with a dismal survival charge for which remedy choices have remained unchanged for over a decade.

Strategies: We use the human Retained Show (ReD) antibody platform (Myrio Therapeutics) to determine a novel single-chain variable fragment (scFv) that recognises epidermal development issue receptor mutant variant III (EGFRvIII), a typical and tumor-specific mutation present in glioblastoma. We use each in vitro practical assays and an in vivo orthotopic xenograft mannequin of glioblastoma to look at the operate of our novel CAR, known as GCT02, focused utilizing murine CAR T cells.

Outcomes: Our EGFRvIII-specific scFv was discovered to be of a lot increased affinity than reported comparators reverse-engineered from monoclonal antibodies. Regardless of the upper affinity, GCT02 CAR T cells kill equivalently however secrete decrease quantities of cytokine. As well as, GCT02-CAR T cells additionally mediate fast and full tumor elimination in vivo.

Conclusion: We current a novel EGFRvIII-specific CAR, with efficient antitumor features each in in vitro and in a xenograft mannequin of human glioblastoma.

XS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV756093 1.0 ug DNA Ask for price

NP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV814303 1.0 ug DNA
EUR 540

DM1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV723183 1.0 ug DNA Ask for price

DWS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV723519 1.0 ug DNA Ask for price

EBM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV723687 1.0 ug DNA Ask for price

EGI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV723933 1.0 ug DNA Ask for price

F7R Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV724761 1.0 ug DNA Ask for price

FND Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV725475 1.0 ug DNA Ask for price

UBB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV716533 1.0 ug DNA
EUR 379.2

WAC Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV716731 1.0 ug DNA
EUR 379.2

AFA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV718317 1.0 ug DNA Ask for price

AIC Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV718395 1.0 ug DNA Ask for price

AOM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV718869 1.0 ug DNA Ask for price

ATD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV719175 1.0 ug DNA Ask for price

GRN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV710665 1.0 ug DNA
EUR 379.2

HGF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV710833 1.0 ug DNA
EUR 379.2

HGF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV710839 1.0 ug DNA
EUR 379.2

HYI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV711145 1.0 ug DNA
EUR 379.2

JTB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV711559 1.0 ug DNA
EUR 379.2

LBH Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV711901 1.0 ug DNA
EUR 379.2

LTF Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712075 1.0 ug DNA
EUR 379.2

LUM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712087 1.0 ug DNA
EUR 379.2

LXN Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712093 1.0 ug DNA
EUR 379.2

MAX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712171 1.0 ug DNA
EUR 379.2

MAX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712177 1.0 ug DNA
EUR 379.2

MAX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712183 1.0 ug DNA
EUR 379.2

MAX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712189 1.0 ug DNA
EUR 379.2

MPI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712369 1.0 ug DNA
EUR 379.2

NF2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712735 1.0 ug DNA
EUR 379.2

NMI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712777 1.0 ug DNA
EUR 379.2

OAT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712987 1.0 ug DNA
EUR 379.2

OS9 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV713023 1.0 ug DNA
EUR 379.2

JBS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730197 1.0 ug DNA Ask for price

JPD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730203 1.0 ug DNA Ask for price

KFM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730353 1.0 ug DNA Ask for price

KMS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730473 1.0 ug DNA Ask for price

KSS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730749 1.0 ug DNA Ask for price

KWE Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730755 1.0 ug DNA Ask for price

LCO Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV730887 1.0 ug DNA Ask for price

GTS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV727149 1.0 ug DNA Ask for price

HMI Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV728379 1.0 ug DNA Ask for price

HPT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV728727 1.0 ug DNA Ask for price

HTL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV728973 1.0 ug DNA Ask for price

CHR Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721173 1.0 ug DNA Ask for price

CMM Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721407 1.0 ug DNA Ask for price

CNC Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721455 1.0 ug DNA Ask for price

COP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721599 1.0 ug DNA Ask for price

CRG Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721785 1.0 ug DNA Ask for price

CRS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721797 1.0 ug DNA Ask for price

CSE Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721839 1.0 ug DNA Ask for price

CTD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV721923 1.0 ug DNA Ask for price

DCE Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV722337 1.0 ug DNA Ask for price

DDU Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV722361 1.0 ug DNA Ask for price

RGR Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV714307 1.0 ug DNA
EUR 379.2

Sp2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV715321 1.0 ug DNA
EUR 379.2

Sp2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV715327 1.0 ug DNA
EUR 379.2

Syk Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV715609 1.0 ug DNA
EUR 379.2

TK1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV715879 1.0 ug DNA
EUR 379.2

TTK Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV716389 1.0 ug DNA
EUR 379.2

GCY Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV726561 1.0 ug DNA Ask for price

PIP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV713473 1.0 ug DNA
EUR 379.2

PTS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV713989 1.0 ug DNA
EUR 379.2

CCT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV720669 1.0 ug DNA Ask for price

CCV Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV720723 1.0 ug DNA Ask for price

CRP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV703483 1.0 ug DNA
EUR 540

NPL Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV703699 1.0 ug DNA
EUR 540

FGA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV704107 1.0 ug DNA
EUR 540

NNT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV704257 1.0 ug DNA
EUR 540

MSD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV734289 1.0 ug DNA Ask for price

NSX Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV736497 1.0 ug DNA Ask for price

NT3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV736503 1.0 ug DNA Ask for price

OAP Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV736659 1.0 ug DNA Ask for price

OED Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV736707 1.0 ug DNA Ask for price

PIH Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV740319 1.0 ug DNA Ask for price

PRD Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV741573 1.0 ug DNA Ask for price

PRS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV741753 1.0 ug DNA Ask for price

SDU Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV748131 1.0 ug DNA Ask for price

SEA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV748137 1.0 ug DNA Ask for price

ST2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV749655 1.0 ug DNA
EUR 686.4

ST3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV749661 1.0 ug DNA Ask for price

ST4 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV749673 1.0 ug DNA Ask for price

ST8 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV749691 1.0 ug DNA Ask for price

RP6 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV747189 1.0 ug DNA Ask for price

RSS Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV747633 1.0 ug DNA Ask for price

S11 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV747687 1.0 ug DNA Ask for price

S12 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV747693 1.0 ug DNA Ask for price

PDR Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV739875 1.0 ug DNA Ask for price

MAA Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV732369 1.0 ug DNA Ask for price

MCO Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV732645 1.0 ug DNA Ask for price

MEB Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV732675 1.0 ug DNA Ask for price

Leave a Reply

Your email address will not be published. Required fields are marked *