glucose uptake assay protocol, Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Fatty acid β-oxidation is a key metabolic pathway to fulfill the power calls for of the liver and supply substrates and cofactors for added processes, akin to ketogenesis and gluconeogenesis, that are important to take care of whole-body glucose homeostasis and help extra-hepatic organ perform within the fasted state. Fatty acid β-oxidation happens inside the mitochondria and…

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Tyrosine hydroxylase: purification from PC-12 cells, characterization and production of antibodies.

Tyrosine hydroxylase: purification from PC-12 cells, characterization and production of antibodies.

Tyrosine hydroxylase has been purified to homogeneity from cultured PC-12 cells. The protein migrates as a single band with a molecular weight of 60,000 on sodium dodecyl sulfate polyacrylamide electrophoresis. Two-dimensional electrophoresis of the pure enzyme resolves three spots (every with molecular weights of 60,000) with isoelectric factors of 5.4, 5.Eight and 5.9. This cost…

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Hippeastrum hybrid Lectin (HHL)

Product name Hybrid Hippeastrum Lectin Full Product Name Hybrid Hippeastrum Lectin (HHL, Lectin Amaryllis, AL) (Biotin) Purity / Purification Degree in Molecular Biology Shape / Format Supplied as a powder in 10 mM HEPES, pH 7.5, 0.15 M sodium chloride, 0.08% sodium azide. Reconstitute with 1 ml of dH2O. Labeled with biotin. Preparation and storage…

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