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ATE1 inhibits liver cancer progression through RGS5-mediated suppression of Wnt/β-catenin signaling
- Lieven
- 0
Arginyltransferase (ATE1) performs vital roles in lots of organic features together with cardiovascular growth, angiogenesis, adipogenesis, muscle contraction, and metastasis of most cancers. Nonetheless, the position of ATE1 in hepatocellular carcinoma (HCC) stays unknown. On this research, we discover that ATE1 performs an important position in progress and malignancy of liver most cancers.
ATE1 expression is considerably decreased in human HCC samples in comparison with regular liver tissue. Moreover, low ATE1 expression is correlated with aggressive clinicopathological options and is an unbiased poor prognostic issue for total survival and disease-free survival of HCC sufferers. Lentivirus-mediated ATE1 knockdown considerably promoted liver most cancers progress, migration and illness development in vitro and in vivo.
Opposing outcomes had been noticed when ATE1 was up-regulated. Mechanistically, ATE1 accelerated the degradation of β-catenin and inhibited Wnt signaling by regulating turnover of Regulator Of G Protein Signaling 5 (RGS5). Loss- and gain-of-function assays confirmed that RGS5 was a key effector of ATE1-mediated regulation of Wnt signaling.
Additional research indicated that RGS5 could be concerned in regulating the exercise of GSK3-β, a vital element of the cytoplasmic destruction complicated. Remedy with a GSK inhibitor (CHIR99021) cooperated with ablation of ATE1 or RGS5 overexpression to advertise Wnt/β-catenin signaling, however overexpression of ATE1 or RGS5 knockdown didn’t reverse the impact of GSK inhibitor. Implications: ATE1 inhibits liver most cancers development by suppressing Wnt/β-catenin signaling and may function a probably useful prognostic biomarker for HCC.
AGEs promote calcification of HASMCs by mediating Pi3k/AKT-GSK3β signaling
This research aimed to research the consequences of superior glycation finish merchandise (AGEs) on the calcification of human arterial easy muscle cells (HASMCs) and to discover whether or not AGEs can promote the calcification of HASMCs by activating the phosphoinositide 3-kinase (PI3K)/AKT-glycogen synthase kinase Three beta (GSK3-β) axis. Cultured HASMCs had been divided into 5 teams: clean management group, dimethyl sulfoxide (automobile) group, AGEs group, LY294002 (AKT inhibitor) group, and TWS119 (GSK3-β inhibitor) group.
Cells had been pretreated with both automobile, LY294002, or TWS119 for two hours adopted by incubation with AGEs (25 μg/mL) for five days, and the expression ranges of proteins in every group had been analyzed by western blotting. AGE therapy promoted HASMC calcification, which coincided with elevated expression of p-AKT and p-GSK3-β (serine 9).
Additionally, AGEs upregulated the expression of osteoprotegerin and bone morphogenetic protein, and these results had been suppressed by LY294002 however enhanced by TWS119. In conclusion, AGEs promote calcification of HASMCs, and this impact is ameliorated by inhibition of AKT exercise however potentiated by inhibition of GSK3-β exercise. Therefore, AGEs set off HASMC calcification by regulating PI3K/AKT-GSK3-β signaling.
GSK3 Inhibitor-Induced Dentinogenesis Utilizing a Hydrogel
Small-molecule medication concentrating on glycogen synthase kinase 3 (GSK3) as inhibitors of the protein kinase exercise are capable of stimulate reparative dentine formation. To develop this method right into a viable medical therapy for uncovered pulp lesions, we synthesized a novel, small-molecule noncompetitive adenosine triphosphate (ATP) drug that may be included right into a biodegradable hydrogel for placement by syringe into the tooth.
This new drug, named NP928, belongs to the thiadiazolidinone (TDZD) household and has equal exercise to related medication of this household equivalent to tideglusib. Nonetheless, NP928 is extra water soluble than different TDZD medication, making it extra appropriate for direct supply into pulp lesions.
We have now beforehand reported that biodegradable marine collagen sponges can efficiently ship TDZD medication to pulp lesions, however this entails in-theater preparation of the fabric, which isn’t best in a medical context.
To enhance surgical dealing with and supply, right here we included NP928 right into a particularly tailor-made hydrogel that may be positioned by syringe right into a broken tooth. This hydrogel is predicated on biodegradable hyaluronic acid and might be gelled in situ upon dental blue gentle publicity, equally to different frequent dental supplies.
NP928 launched from hyaluronic acid-based hydrogels upregulated Wnt/β-catenin exercise in pulp stem cells and fostered reparative dentine formation in comparison with marine collagen sponges delivering equal concentrations of NP928. This drug-hydrogel mixture has the potential to be quickly developed right into a therapeutic process that’s amenable to normal dental follow.
Direct P70S6K1 inhibition to exchange dexamethasone in synergistic mixture with MCL-1 inhibition in a number of myeloma
Novel mixture therapies have markedly improved the lifespan of sufferers with a number of myeloma (MM), however drug resistance and illness relapse stay main medical issues. Dexamethasone and different glucocorticoids are a cornerstone of standard and new mixture therapies for MM, though their use is accompanied by critical unwanted side effects.
We aimed to uncover drug combos that act in synergy and, as such, enable decreased dosing whereas remaining efficient. Dexamethasone and the myeloid cell leukemia 1 (MCL-1) inhibitor S63845 (MCL-1i) proved probably the most potent mixture in our lethality display screen and induced apoptosis of human myeloma cell strains (HMCLs) that was 50% greater in contrast with an additive drug impact.
Kinome evaluation of dexamethasone-treated HMCLs revealed a discount in serine/threonine peptide phosphorylation, which was predicted to consequence from decreased Akt exercise. Biochemical methods confirmed no dexamethasone-induced results on FOXO protein or GSK3 however did present a 50% discount in P70S6K phosphorylation, downstream of the Akt-mTORC1 axis.
Changing dexamethasone by the P70S6K1 isoform-specific inhibitor PF-4708671 (S6K1i) revealed related and statistically important synergistic apoptosis of HMCLs together with MCL-1i. Apparently, apoptosis induced by the P70S6K1i and MCL-1i mixture was more-than-additive in all 9 main MM samples examined; this impact was noticed for six of 9 samples with the dexamethasone and MCL-1i mixture.
GSK3B antibody |
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38616-100ul | SAB | 100ul | EUR 302.4 |
GSK3B Antibody |
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CSB-PA939628- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
GSK3B Antibody |
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CSB-PA939628-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
GSK3B Antibody |
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1-CSB-PA009963GA01HU | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat, Pig. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
GSK3B Antibody |
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1-CSB-PA009963LA01HU | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:2000-1:10000, IF:1:50-1:500 |
GSK3B Antibody |
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E036918 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E19-6806 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E19-7231 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E10-30006 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E10-30705 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E10-30705T | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
Gsk3b Antibody |
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F44221-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
Description: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity). |
Gsk3b Antibody |
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F44221-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity). |
GSK3B Antibody |
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F51046-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
Description: GSK3B is a constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. May also mediate the development of insulin resistance by regulating activation of transcription factors. [UniProt] |
GSK3B Antibody |
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F51046-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: GSK3B is a constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. May also mediate the development of insulin resistance by regulating activation of transcription factors. [UniProt] |
GSK3B Antibody |
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E220184-1 | EnoGene | 50μg/50μl | EUR 145 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E220184-2 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E92081 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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E93174 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
GSK3B antibody |
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E39-03673 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Antibody |
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1-CSB-PA002848 | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.ELISA:1/20000 |
GSK3B Antibody |
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1-CSB-PA009038 | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000 |
GSK3B Antibody |
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36918 | SAB | 100ul | EUR 319 |
GSK3B Antibody |
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36918-100ul | SAB | 100ul | EUR 302.4 |
GSK3B antibody |
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70R-15005 | Fitzgerald | 100 ul | EUR 497 |
Description: Rabbit polyclonal GSK3B antibody |
GSK3B antibody |
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70R-17615 | Fitzgerald | 50 ul | EUR 289 |
Description: Rabbit polyclonal GSK3B antibody |
GSK3b antibody |
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70R-11869 | Fitzgerald | 100 ug | EUR 357 |
Description: Rabbit polyclonal GSK3b antibody |
GSK3B Antibody |
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BF0695 | Affbiotech | 200ul | EUR 540 |
GSK3B Antibody |
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BF0695-100ul | Affinity Biosciences | 100ul | EUR 350 |
GSK3B Antibody |
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BF0695-200ul | Affinity Biosciences | 200ul | EUR 450 |
GSK3B Antibody |
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BF0695-50ul | Affinity Biosciences | 50ul | EUR 250 |
GSK3B Antibody |
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ABD6806 | Lifescience Market | 100 ug | EUR 525.6 |
GSK3B Antibody |
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ABD7231 | Lifescience Market | 100 ug | EUR 525.6 |
GSK3B antibody |
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CAF50144-100ug | Biomatik Corporation | 100ug | EUR 312 |
GSK3B Antibody |
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1-CSB-PA080166 | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC;WB:1:1000-2000.IHC:1:200-500 |
GSK3B Antibody |
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GWB-MT481D | GenWay Biotech | 50ug | Ask for price |
GSK3A / GSK3B Antibody |
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20-abx328487 | Abbexa |
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GSK3A/GSK3B Antibody |
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1-CSB-PA002838 | Cusabio |
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Description: A polyclonal antibody against GSK3A/GSK3B. Recognizes GSK3A/GSK3B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
GSK3A / GSK3B Antibody |
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abx328487-100g | Abbexa | 100 µg | EUR 250 |
GSK3A / GSK3B Antibody |
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abx328487-50g | Abbexa | 50 µg | EUR 187.5 |
Mouse anti GSK3b(pS9) Monoclonal Antibody |
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TA354463 | Origene Technologies GmbH | 100 µg | Ask for price |
Rabbit anti GSK3b(pS9) Polyclonal Antibody |
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TA354486 | Origene Technologies GmbH | 100 µg | Ask for price |
GSK3B (pY216) Antibody |
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20-abx123289 | Abbexa |
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GSK3B (pY216) Antibody |
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20-abx000312 | Abbexa |
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GSK3B (pSer9) Antibody |
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abx243027-96tests | Abbexa | 96 tests | EUR 287.5 |
GSK3B (pY216) Antibody |
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abx123289-100l | Abbexa | 100 µl | EUR 200 |
GSK3B (pY216) Antibody |
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abx123289-1ml | Abbexa | 1 ml | EUR 475 |
GSK3B (pY216) Antibody |
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abx123289-200l | Abbexa | 200 µl | EUR 325 |
GSK3B Polyclonal Antibody |
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E96164 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Polyclonal Antibody |
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E911360 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Polyclonal Antibody |
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E911578 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Polyclonal Antibody |
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E916868 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
GSK3B Conjugated Antibody |
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C36918 | SAB | 100ul | EUR 476.4 |
GSK3B Conjugated Antibody |
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C38353 | SAB | 100ul | EUR 476.4 |
GSK3B Polyclonal Antibody |
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A62706 | EpiGentek |
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GSK3B Polyclonal Antibody |
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A72446 | EpiGentek | 50 μl | EUR 408 |
GSK3B Polyclonal Antibody |
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A72447 | EpiGentek | 50 μl | EUR 408 |
GSK3B Polyclonal Antibody |
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A72448 | EpiGentek | 50 μl | EUR 408 |
GSK3B Polyclonal Antibody |
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A72450 | EpiGentek | 50 μl | EUR 557 |
Anti-GSK3 beta/GSK3B Antibody Picoband™ |
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A00791-3 | BosterBio | 10ug | EUR 154 |
Description: Western blot, 0.25-0.5 μg/ml, Human;_x000D_Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human;_x000D_Direct ELISA, 0.1-0.5 μg/ml, Human |
GSK3 beta Antibody / GSK3B |
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F54007-0.1ML | NSJ Bioreagents | 0.1 ml | EUR 322.15 |
Description: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. [UniProt] |
Rabbit anti GSK3b(Paired S9) Polyclonal Antibody |
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TA354454 | Origene Technologies GmbH | 100 µg | Ask for price |
GSK3B (Ab-9) Antibody |
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CSB-PA120951- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against GSK3B (Ab-9). Recognizes GSK3B (Ab-9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
GSK3B (Ab-9) Antibody |
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CSB-PA120951-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against GSK3B (Ab-9). Recognizes GSK3B (Ab-9) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
GSK3B (pS21 / 29) Antibody |
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abx031864-400ul | Abbexa | 400 ul | EUR 627.6 |
GSK3B (pS21 / 29) Antibody |
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abx031864-80l | Abbexa | 80 µl | EUR 343.2 |
GSK3B (pS21 / 29) Antibody |
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abx031864-400l | Abbexa | 400 µl | EUR 525 |
ARP97287_P050 - GSK3B Antibody-C-terminal region |
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ARP97287_P050 | Aviva Systems Biology | 100ul | EUR 389 |
ARP97287_P050-25UL - GSK3B Antibody - C-terminal region |
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ARP97287_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP54644_P050-25UL - GSK3B Antibody - C-terminal region |
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ARP54644_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
GSK3B Antibody, HRP conjugated |
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1-CSB-PA009963LB01HU | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
GSK3B Antibody, FITC conjugated |
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1-CSB-PA009963LC01HU | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
OAPA00251-5UG - GSK3B Antibody |
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OAPA00251-5UG | Aviva Systems Biology | 5ug | EUR 69 |
GSK3A / GSK3B (pY279 / 216) Antibody |
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20-abx328488 | Abbexa |
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GSK3A / GSK3B (pY279 / 216) Antibody |
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abx333212-100ul | Abbexa | 100 ul | EUR 560.4 |
GSK3A / GSK3B (pY279 / 216) Antibody |
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abx328488-100g | Abbexa | 100 µg | EUR 250 |
GSK3A / GSK3B (pY279 / 216) Antibody |
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abx328488-50g | Abbexa | 50 µg | EUR 187.5 |
GSK3A / GSK3B (pY279 / 216) Antibody |
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abx333212-100g | Abbexa | 100 µg | Ask for price |
GSK3A / GSK3B (pY279 / 216) Antibody |
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abx333212-20g | Abbexa | 20 µg | EUR 412.5 |
GSK3A / GSK3B (pY279 / 216) Antibody |
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abx333212-50g | Abbexa | 50 µg | Ask for price |
ARP54644_P050 - GSK3B antibody - C-terminal region (ARP54644_P050) |
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ARP54644_P050 | Aviva Systems Biology | 100ul | EUR 389 |
OACA03072-50UG - GSK3B Antibody |
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OACA03072-50UG | Aviva Systems Biology | 50ug | EUR 179 |
GSK3B Antibody, Biotin conjugated |
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1-CSB-PA009963LD01HU | Cusabio |
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Description: A polyclonal antibody against GSK3B. Recognizes GSK3B from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
GSK3B Rabbit Polyclonal Antibody |
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53236 | SAB | 100ul | EUR 439 |
OASG03249-100UL - GSK3B Antibody |
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OASG03249-100UL | Aviva Systems Biology | 100ul | EUR 239 |
OAAJ02835-100UL - GSK3B Antibody |
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OAAJ02835-100UL | Aviva Systems Biology | 100ul | EUR 319 |
OAAJ07280-100UL - GSK3B Antibody |
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OAAJ07280-100UL | Aviva Systems Biology | 100uL | EUR 319 |
OAAF01318-100UG - GSK3B Antibody |
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OAAF01318-100UG | Aviva Systems Biology | 100ug | EUR 329 |
OACA03072-100UG - GSK3B Antibody |
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OACA03072-100UG | Aviva Systems Biology | 100ug | EUR 319 |
OASG07740-100UL - GSK3B Antibody |
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OASG07740-100UL | Aviva Systems Biology | 100ul | EUR 269 |
OASG07741-100UL - GSK3B Antibody |
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OASG07741-100UL | Aviva Systems Biology | 100ul | EUR 269 |
OASG07742-100UL - GSK3B Antibody |
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OASG07742-100UL | Aviva Systems Biology | 100ul | EUR 269 |
GSK3B Rabbit Polyclonal Antibody |
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E312222 | EnoGene | 100ug/200ul | EUR 295 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GSK3B Rabbit Polyclonal Antibody |
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E10G05782 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GSK3B Rabbit Polyclonal Antibody |
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E10G10937 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GSK3B Rabbit Polyclonal Antibody |
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E10G12655 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
Rabbit Anti-Human GSK3B (aa 214-218) Polyclonal Antibody |
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CPB-1178RH | Creative Diagnostics | 100 ul | EUR 552 |
GSK3A/GSK3B (pY216 + pY279) Antibody |
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abx243004-96tests | Abbexa | 96 tests | EUR 287.5 |
GSK3B Polyclonal Conjugated Antibody |
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C30706 | SAB | 100ul | EUR 476.4 |
Polyclonal GSK3B / GSK3 Beta Antibody |
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AMM05221G | Leading Biology | 0.05mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GSK3B / GSK3 Beta . This antibody is tested and proven to work in the following applications: |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-A565 | Stressmarq | 0.1ml | EUR 480 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 565. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-A633 | Stressmarq | 0.1ml | EUR 480 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 633. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-A655 | Stressmarq | 0.1ml | EUR 480 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 655. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-A680 | Stressmarq | 0.1ml | EUR 480 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 680. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-A700 | Stressmarq | 0.1ml | EUR 480 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to ATTO 700. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-ALP | Stressmarq | 0.1ml | EUR 472.8 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Alkaline Phosphatase. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-APC | Stressmarq | 0.1ml | EUR 478.8 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to APC . |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-APCCY7 | Stressmarq | 0.1ml | EUR 565.2 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to APC/Cy7. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-DY350 | Stressmarq | 0.1ml | EUR 496.8 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 350. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-DY405 | Stressmarq | 0.1ml | EUR 483.6 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 405. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-DY488 | Stressmarq | 0.1ml | EUR 471.6 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 488. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-DY594 | Stressmarq | 0.1ml | EUR 474 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 594. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-DY633 | Stressmarq | 0.1ml | EUR 468 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Dylight 633. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-HRP | Stressmarq | 0.1ml | EUR 465.6 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to HRP. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-P594 | Stressmarq | 0.1ml | EUR 488.4 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to PE/ATTO 594. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-PCP | Stressmarq | 0.1ml | EUR 478.8 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to PerCP. |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-RPE | Stressmarq | 0.1ml | EUR 476.4 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to RPE . |
Antibody for Human GSK3b (pSer9) |
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SPC-1384D-STR | Stressmarq | 0.1ml | EUR 477.6 |
Description: A polyclonal antibody for GSK3b (pSer9) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human GSK3b around the phosphorylation site of serine 9 (pSer9).. The Antibody is tested and validated for WB, IHC, IP assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions). This GSK3b (pSer9) antibody is conjugated to Streptavidin. |
GSK3A / GSK3B (Ab-279 / 216) Antibody |
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abx332166-100ul | Abbexa | 100 ul | EUR 510 |
GSK3A/GSK3B (Ab-279/216) Antibody |
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CSB-PA255909- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against GSK3A/GSK3B (Ab-279/216). Recognizes GSK3A/GSK3B (Ab-279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
GSK3A/GSK3B (Ab-279/216) Antibody |
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CSB-PA255909-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against GSK3A/GSK3B (Ab-279/216). Recognizes GSK3A/GSK3B (Ab-279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
Rabbit Anti-Human GSK3B Polyclonal Antibody, Phospho-Tyr216 |
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CPB-820RH | Creative Diagnostics | 100 ul | EUR 670.8 |
Mouse Monoclonal Antibody to GSK3B |
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sAP-1054 | Angio Proteomie | 50ug | EUR 270 |
Mouse Monoclonal Antibody to GSK3B |
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sAP-0359 | Angio Proteomie | 50ug | EUR 270 |
Anti-Glycogen synthase kinase 3 B (GSK3B) (Ser9) antibody |
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STJ120111 | St John's Laboratory | 100 µl | EUR 631.2 |
Monoclonal GSK3B Antibody, Clone: 3D6B4 |
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AMM05223G | Leading Biology | 0.1ml | EUR 633.6 |
Description: A Monoclonal antibody against Human GSK3B. The antibodies are raised in Mouse and are from clone 3D6B4. This antibody is applicable in WB and IHC, FC, E |
[KO Validated] GSK3B Polyclonal Antibody |
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30706 | SAB | 100ul | EUR 439 |
[KO Validated] GSK3B Polyclonal Antibody |
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30706-100ul | SAB | 100ul | EUR 302.4 |
[KO Validated] GSK3B Polyclonal Antibody |
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30706-50ul | SAB | 50ul | EUR 224.4 |
Rabbit Anti-GSK3A, GSK3B monoclonal antibody, clone TZ37-16 |
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CABT-L635 | Creative Diagnostics | 100 ul | EUR 932.4 |
GSK3B Polyclonal Antibody, HRP Conjugated |
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A62707 | EpiGentek |
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Rabbit Anti-Human GSK3B Monoclonal Antibody, Clone S10-8H9 |
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DMAB-JX2362057 | Creative Diagnostics | 20 µL, 50 µL, 100 µL | Ask for price |
Description: Rabbit |
GSK3B Polyclonal Antibody, FITC Conjugated |
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A62708 | EpiGentek |
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GSK3B Polyclonal Antibody, Biotin Conjugated |
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A62709 | EpiGentek |
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Rabbit anti Glycogen Synthase Kinase 3b (GSK3b) Polyclonal Antibody |
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TA354389 | Origene Technologies GmbH | 100 µg | Ask for price |
Phospho-GSK3A/GSK3B (Y279/216) Antibody |
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1-CSB-PA000535 | Cusabio |
|
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Description: A polyclonal antibody against Phospho-GSK3A/GSK3B (Y279/216). Recognizes Phospho-GSK3A/GSK3B (Y279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
Phospho-GSK3A/GSK3B (Tyr279/216) Antibody |
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CSB-PA575559- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against Phospho-GSK3A/GSK3B (Tyr279/216). Recognizes Phospho-GSK3A/GSK3B (Tyr279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Phospho-GSK3A/GSK3B (Tyr279/216) Antibody |
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CSB-PA575559-100ul | Cusabio | 100ul | EUR 434.4 |
Description: A polyclonal antibody against Phospho-GSK3A/GSK3B (Tyr279/216). Recognizes Phospho-GSK3A/GSK3B (Tyr279/216) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
OAEC01479-100UG - GSK3b (Ab-9) Antibody |
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OAEC01479-100UG | Aviva Systems Biology | 100ug | EUR 369 |
Polyclonal Mouse Gsk3b Antibody (N-term) |
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APR04027G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Mouse Gsk3b (N-term). This antibody is tested and proven to work in the following applications: |
OASG03245-100UL - GSK3B Antibody (Phospho-Ser9) |
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OASG03245-100UL | Aviva Systems Biology | 100ul | EUR 239 |
OASG03246-100UL - GSK3B Antibody (Phospho-Ser9) |
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OASG03246-100UL | Aviva Systems Biology | 100ul | EUR 239 |
OAGA08182-100UL - GSK3B Antibody (Phospho-Ser9) |
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OAGA08182-100UL | Aviva Systems Biology | 100ul | EUR 369 |
Polyclonal GSK3B / GSK3 Beta Antibody (C-Terminus) |
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AMM05222G | Leading Biology | 0.05mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GSK3B / GSK3 Beta (C-Terminus). This antibody is tested and proven to work in the following applications: |
GSK3B / GSK3 Beta Rabbit Polyclonal (pSer9) Antibody |
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TA317698 | Origene Technologies GmbH | 50 µg | Ask for price |
OAEC00270-50UG - GSK3B Antibody (Phospho-Tyr216) |
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OAEC00270-50UG | Aviva Systems Biology | 50ug | EUR 369 |
GSK3 beta (GSK3B) rabbit polyclonal antibody, Purified |
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AP22887PU-N | Origene Technologies GmbH | 50 µg | Ask for price |
OABF00015-100UL - GSK3B Antibody (OABF00015-100UL) |
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OABF00015-100UL | Aviva Systems Biology | 100ul | EUR 409 |
glycogen synthase kinase 3 beta (GSK3B) Antibody |
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E301022 | EnoGene | 100ug/200ul | EUR 275 |
Description: Available in various conjugation types. |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx318062 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx328988 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx329709 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx332790-100ul | Abbexa | 100 ul | EUR 510 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx330217 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx332014-100ul | Abbexa | 100 ul | EUR 510 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx037712-100ug | Abbexa | 100 ug | EUR 469.2 |
Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx172635 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx172636 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx176674 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx176675 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx125048 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx125336 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx125917 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233673-100ug | Abbexa | 100 ug | EUR 577.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233674-100ug | Abbexa | 100 ug | EUR 577.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233675-100ug | Abbexa | 100 ug | EUR 661.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233676-100ug | Abbexa | 100 ug | EUR 610.8 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx224101-100ug | Abbexa | 100 ug | EUR 493.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx112808 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx147405-100ug | Abbexa | 100 ug | EUR 526.8 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx004714 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx010856-100ul | Abbexa | 100 ul | EUR 493.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx001694 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx002277 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (Gsk3b) Antibody |
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abx028354-400ul | Abbexa | 400 ul | EUR 627.6 |
Glycogen Synthase Kinase 3 Beta (Gsk3b) Antibody |
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abx028354-80l | Abbexa | 80 µl | EUR 343.2 |
Rabbit polyclonal Mouse Gsk3b Antibody (N-term) |
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TA324570 | Origene Technologies GmbH | 400 µl | Ask for price |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx002277-100l | Abbexa | 100 µl | EUR 475 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx002277-20l | Abbexa | 20 µl | EUR 200 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx002277-50l | Abbexa | 50 µl | EUR 325 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx004714-100l | Abbexa | 100 µl | EUR 400 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx004714-20l | Abbexa | 20 µl | EUR 175 |
×
Toxicity on stem and progenitor cell subsets remained minimal. Mixed, our outcomes present a powerful rationale for mixture remedies utilizing the P70S6K inhibitor in MM. Direct and particular inhibition of P70S6K may present an answer for sufferers ineligible or insensitive to dexamethasone or different glucocorticoids.
Tags: egfp sequence gfp lc3 grna synthesis lentiguide minimal cmv promoter sequence pbad promoter sequence pbad33 pbluescript ks pbluescript sk pbs plasmid pcdna vector pcdna3 1 zeo pcr8 gw topo pet100 pet15b pet28 vector pet42b pgadt7 pgl2 basic pqcxin pqe30 vector prep4 plasmid ptrchis ptrex ptxb1 puc vector puc19 plasmid map puc57 puc57 vector topo pcr4 xhoi restriction site zero blunt topo sequence