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Antiviral Effectivity of Favipiravir Against Peste Des Petits Ruminants Virus Is Mediated by the JAK/STAT and PI3K/AKT Pathways
Lieven
- 0
Peste des petits ruminants virus (PPRV), belonging to the genus Morbillivirus within the household Paramyxoviridae, causes extreme infectious illness in small ruminants and has been quickly spreading in lots of elements of Africa, the Center East, and Asia.
Though vaccination is taken into account to be an efficient technique of controlling PPR, the heat-sensitive nature of the vaccines towards PPRV enormously limits their utility in areas with a sizzling local weather. Within the current research, we investigated the anti-PPRV results of favipiravir and sought to determine the underlying mechanisms in vitro utilizing the Vero cell line.
MTT assays, Western blotting, oblique immunofluorescence assays, virus plaque formation assays, and qRT-PCR had been used to evaluate the consequences of favipiravir on the life cycle of PPRV and the expression of RNA-dependent RNA polymerase (RdRp). Moreover, the expression ranges of JAK1, STAT1, phosphorylated (p)-STAT1, PI3K, AKT, and p-AKT, in addition to these of signaling molecules appearing downstream of the JAK/STAT and PI3K/AKT signaling pathways, had been decided by Western blotting and qRT-PCR.
The outcomes indicated that, in PPRV-infected, favipiravir-treated Vero cells, the attachment, invasion, replication, and launch of PPRV had been considerably inhibited, as was the expression of RdRp, in comparison with that in untreated PPRV-infected cells.
Moreover, in favipiravir-treated cells, the expression of JAK1 and STAT1 was downregulated, whereas that of p-STAT1 was considerably upregulated. Equally, the expression ranges of PKR, IRF9, ISG54, and MxA proteins which are related to innate antiviral exercise in host cells had been additionally markedly elevated. Furthermore, with favipiravir therapy, the expression of PI3K and p-AKT and the p-AKT/AKT ratio had been considerably decreased, whereas the expression of AKT was noticeably upregulated.
The expression of GSK3, NF-κB p65, p-NF-κB p65, and BAD was additionally elevated with favipiravir therapy, whereas the expression of CREB, p-CREB, p-GSK3, and Bcl-2 was barely decreased. As well as, all of the p-GSK3/GSK3, p-CREB/CREB, p-NF-κB/NF-κB, and p-BAD/BAD ratios had been considerably decreased in favipiravir-treated cells.
These outcomes implied that the antiviral effectivity of favipiravir towards PPRV is mediated by the JAK/STAT and PI3K/AKT pathways and that favipiravir has potential to be used as an efficient antiviral agent towards PPRV.
Glycogen synthase kinase-3β inactivation promotes cervical most cancers development, invasion, and drug resistance
Human papillomavirus (HPV) infection-dependent cervical most cancers is without doubt one of the most typical gynecological cancers and infrequently turns into aggressive, with fast proliferation, invasion/migration, and drug resistance.
Right here, 135 recent human CSCC tissue specimens, comprising: 21 adjoining normals (AN), 30 cervical-intraepithelial-neoplasia (CIN1-3 ), 45 CSCC, and 39 medicine (chemo-radiation)-resistant cervical tumor (DRCT) tissues had been included.
HPV-positive (HeLa, SiHa), HPV-negative (C33A), and cisplatin-resistant (CisR-HeLa/-SiHa/-C33A) cell strains had been used for in vitro research. HPV16/18 oncoproteins E6/E7, pERK1/2, and glycogen synthase kinase-3 (GSK3) and the matrix metalloproteinases (MMPs) MMP-9/-2 had been assessed utilizing immunohistochemistry, WB, and gelatin zymography.
HPV16/18 an infection was noticed in 16.7% of the CIN1-3 , 77.8% of the CSCC, and 89.7% of DRCT samples. Whole and inactive GSK3β expressions had been related to total CSCC development (p = 0.039 and p = 0.024, respectively) and chemoresistance (p = 0.004 and p = 0.014, respectively).
Constructive correlations had been noticed, between the expression of E6 and pGSK3β expression (p = 0.013); E6 and CSCC development (p<0.0001)/ drug resistance (p = 0.0001). CisR-HeLa/-SiHa was extra depending on pGSK3β, and activation of GSK3 by SMIs (iAkt), therapy with nimbolide, or knockdown of E6/E7 decreased cisplatin-resistance and promoted apoptosis. Therefore, the activation of GSK3β with nimbolide and iAkt may be exploited for therapeutic interventions of cervical most cancers. This text is protected by copyright. All rights reserved.
G protein-coupled receptor kinase 2 is crucial to allow vasoconstrictor-mediated arterial clean muscle proliferation
Hypertension is related to elevated manufacturing and circulation of vasoconstrictors, leading to enhanced signalling by their cognate G protein-coupled receptors (GPCR). Extended vasoconstrictor GPCR signalling will increase arterial contraction and stimulates signalling pathways that promote vascular clean muscle cell (VSMC) proliferation, contributing to the event of atherosclerotic plaques, re-stenosis lesions and vascular remodelling.
GPCR signalling by phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) promotes VSMC proliferation. In VSMC, G protein-coupled receptor kinase 2 (GRK2) is thought to manage quite a few vasoconstrictor GPCRs and their downstream signalling pathways.
As GRK2 is implicated in controlling numerous elements of mobile development, we examined whether or not GRK2 might have an effect on VSMC proliferation. Utilizing two indices of cell development, we present that PI3K inhibition and depletion of GRK2 expression produced the same ablation of pro-proliferative vasoconstrictor-stimulated VSMC development.
Moreover, GRK2-knockdown ablated the sustained part of endothelin-1 and angiotensin-II-stimulated Akt phosphorylation, while the height (5 min) part was unaffected. Conversely, the GRK2 inhibitor compound 101 didn’t have an effect on vasoconstrictor-driven Akt phosphorylation.
Vasoconstrictor-stimulated phosphorylation of the Akt substrates GSK3α and GSK3β was ablated following RNAi-mediated GRK2 depletion, or after PI3K inhibition. Furthermore, GRK2 knockdown prevented endothelin-1 and angiotensin-II from rising cyclin D1 expression.
These knowledge counsel GRK2 expression is crucial to facilitate vasoconstrictor-driven VSMC proliferation by its potential to advertise environment friendly extended PI3K-Akt signalling, and thus relieve the GSK3-mediated block on cell biking. Contemplating VSMC GRK2 expression will increase early within the improvement of hypertension, this highlights the potential for GRK2 to advertise VSMC development and exacerbate hypertensive pathophysiological vascular remodelling.
Publicity to Perfluoro-Octanoic Acid Related With Upstream Uncoupling of the Insulin Signaling in Human Hepatocyte Cell Line
Perfluoro-alkyl substances (PFAS) are chemical pollution with prevalent stability and environmental persistence. Publicity to PFAS, notably perfluoro-octanoic acid (PFOA), has been related to elevated diabetes-related cardiovascular mortality in topics residing areas of excessive environmental contamination, nevertheless the precise pathogenic mechanism stays elusive.

Right here we used HepG2 cells, an in vitro mannequin of human hepatocyte, to research the potential position of PFOA publicity within the alteration of hepatic glucose metabolism. HepG2 cells had been uncovered for 24 hours to PFOA at rising focus from Zero to 1000 ng/mL after which stimulated with 100 nm Insulin (Ins).
The ensuing impact on glycogen synthesis, glucose uptake and Glut-Four glucose transporter translocation was then evaluated by, respectively, Periodic Acid Schiff (PAS) staining, 2-deoxyglucose (2-DG) uptake assay and immunofluorescence.
Publicity to PFOA was related to decreased glycogen synthesis and glucose uptake, at focus equal or higher than, respectively, 0,1 ng/mL and 10 ng/mL, with parallel impaired membrane translocation of Glut-Four upon Ins stimulation. Western blot evaluation confirmed early uncoupling of Insulin Receptor (InsR) activation from the downstream Akt and GSK3 phosphorylation.

Computational docking evaluation disclosed the potential stabilizing impact of PFOA on the advanced between InsR and GM3 ganglioside, beforehand proven to be related to the low grade persistent inflammation-related insulin resistance.
Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody |
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20-abx210327 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody |
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20-abx210328 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3a) Antibody |
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20-abx130116 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (Gsk3a) Antibody |
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abx028353-400ul | Abbexa | 400 ul | EUR 627.6 |
Glycogen Synthase Kinase 3 Alpha (Gsk3a) Antibody |
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abx028353-80l | Abbexa | 80 µl | EUR 343.2 |
Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody |
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abx331993-100ul | Abbexa | 100 ul | EUR 510 |
Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody |
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abx332236-100ul | Abbexa | 100 ul | EUR 510 |
Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody |
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20-abx329707 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody (HRP) |
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20-abx316098 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody (FITC) |
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20-abx316099 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3A) Antibody (Biotin) |
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20-abx316100 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat) |
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4-PAA630Ra01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human) |
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4-PAA630Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a) |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse) |
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4-PAA630Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a) |
Recombinant Glycogen Synthase Kinase 3 Alpha (GSK3a) |
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4-RPA630Hu01 | Cloud-Clone |
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Description: Recombinant Human Glycogen Synthase Kinase 3 Alpha expressed in: E.coli |
Recombinant Glycogen Synthase Kinase 3 Alpha (GSK3a) |
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4-RPA630Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Glycogen Synthase Kinase 3 Alpha expressed in: E.coli |
Recombinant Glycogen Synthase Kinase 3 Alpha (GSK3a) |
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4-RPA630Ra01 | Cloud-Clone |
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Description: Recombinant Rat Glycogen Synthase Kinase 3 Alpha expressed in: E.coli |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), PE |
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4-PAA630Ra01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with PE. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), APC |
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4-PAA630Ra01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with APC. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), Cy3 |
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4-PAA630Ra01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with Cy3. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), HRP |
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4-PAA630Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with HRP. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), PE |
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4-PAA630Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with PE. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), PE |
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4-PAA630Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with PE. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), FITC |
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4-PAA630Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with FITC. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) Protein |
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20-abx167726 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), APC |
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4-PAA630Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with APC. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), Cy3 |
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4-PAA630Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with Cy3. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), FITC |
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4-PAA630Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with FITC. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), HRP |
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4-PAA630Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with HRP. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), APC |
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4-PAA630Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with APC. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), Cy3 |
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4-PAA630Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with Cy3. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), FITC |
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4-PAA630Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with FITC. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), HRP |
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4-PAA630Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with HRP. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) Protein |
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20-abx066875 | Abbexa |
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Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a) Protein |
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20-abx066876 | Abbexa |
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Glycogen Synthase Kinase 3 Alpha Phospho-Ser21 (GSK3A pS21) Antibody |
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abx333153-100ul | Abbexa | 100 ul | EUR 560.4 |
Glycogen Synthase Kinase 3 Alpha Phospho-Ser21 (GSK3A pS21) Antibody |
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20-abx329708 | Abbexa |
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Rat Glycogen Synthase Kinase 3 Alpha (GSK3A) CLIA Kit |
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abx197051-96tests | Abbexa | 96 tests | EUR 990 |
Rat Glycogen Synthase Kinase 3 alpha (GSK3a) CLIA Kit |
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20-abx491794 | Abbexa |
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Rat Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA Kit |
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20-abx155612 | Abbexa |
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Dog Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx355435-96tests | Abbexa | 96 tests | EUR 1111.2 |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx256173-96tests | Abbexa | 96 tests | EUR 801.6 |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx573249-96tests | Abbexa | 96 tests | EUR 801.6 |
Pig Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx360633-96tests | Abbexa | 96 tests | EUR 990 |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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DLR-GSK3a-Ra-48T | DL Develop | 48T | EUR 609.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in samples from tissue homogenates, cell lysates or other biological fluids. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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DLR-GSK3a-Ra-96T | DL Develop | 96T | EUR 793.2 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in samples from tissue homogenates, cell lysates or other biological fluids. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Ra-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5552.14 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in tissue homogenates, cell lysates and other biological fluids. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Ra-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 562.42 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in tissue homogenates, cell lysates and other biological fluids. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Ra-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 752.02 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in tissue homogenates, cell lysates and other biological fluids. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Ra-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3024.07 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in tissue homogenates, cell lysates and other biological fluids. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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4-SEA630Ra | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RDR-GSK3a-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 640.8 |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RDR-GSK3a-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 890.4 |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 613.2 |
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 850.8 |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), Biotinylated |
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4-PAA630Ra01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with Biotin. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Rat), APC-Cy7 |
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4-PAA630Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with APC-Cy7. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) CLIA Kit |
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abx197049-96tests | Abbexa | 96 tests | EUR 990 |
Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a) CLIA Kit |
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abx197050-96tests | Abbexa | 96 tests | EUR 990 |
Human Glycogen Synthase Kinase 3 alpha (GSK3a) CLIA Kit |
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20-abx491793 | Abbexa |
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Rat Glycogen synthase kinase-3 alpha,Gsk3a ELISA Kit |
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YLA1262RA-48T | Shanghai YL Biotech | 48T | EUR 465 |
Rat Glycogen synthase kinase-3 alpha,Gsk3a ELISA Kit |
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YLA1262RA-96T | Shanghai YL Biotech | 96T | EUR 600 |
Mouse Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx513946-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx254668-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA Kit |
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20-abx151753 | Abbexa |
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Sheep Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx364410-96tests | Abbexa | 96 tests | EUR 1111.2 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx351969-96tests | Abbexa | 96 tests | EUR 904.8 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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DLR-GSK3a-Hu-48T | DL Develop | 48T | EUR 574.8 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in samples from tissue homogenates or other biological fluids. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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DLR-GSK3a-Hu-96T | DL Develop | 96T | EUR 745.2 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in samples from tissue homogenates or other biological fluids. |
Human Glycogen synthase kinase- 3 alpha, GSK3A ELISA KIT |
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ELI-02620h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Glycogen synthase kinase- 3 alpha, Gsk3a ELISA KIT |
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ELI-02621m | Lifescience Market | 96 Tests | EUR 1038 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5128.02 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in Tissue homogenates and other biological fluids. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 527.48 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in Tissue homogenates and other biological fluids. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 702.12 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in Tissue homogenates and other biological fluids. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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SEA630Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2799.54 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in Tissue homogenates and other biological fluids. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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4-SEA630Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Glycogen Synthase Kinase 3 Alpha (GSK3a) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RDR-GSK3a-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 600 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RDR-GSK3a-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 830.4 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 573.6 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit |
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RD-GSK3a-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 794.4 |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), Biotinylated |
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4-PAA630Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with Biotin. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), Biotinylated |
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4-PAA630Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with Biotin. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Human), APC-Cy7 |
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4-PAA630Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with APC-Cy7. |
Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody (Mouse), APC-Cy7 |
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4-PAA630Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a). This antibody is labeled with APC-Cy7. |
Monkey Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx358831-96tests | Abbexa | 96 tests | EUR 990 |
Rabbit Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx363672-96tests | Abbexa | 96 tests | EUR 990 |
Mouse Glycogen synthase kinase-3 alpha,Gsk3a ELISA Kit |
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YLA1429MO-48T | Shanghai YL Biotech | 48T | EUR 465 |
Mouse Glycogen synthase kinase-3 alpha,Gsk3a ELISA Kit |
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YLA1429MO-96T | Shanghai YL Biotech | 96T | EUR 600 |
Human Glycogen synthase kinase-3 alpha,Gsk3a ELISA Kit |
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YLA3650HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Glycogen synthase kinase-3 alpha,Gsk3a ELISA Kit |
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YLA3650HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Chicken Glycogen Synthase Kinase 3 Alpha (GSK3A) ELISA Kit |
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abx355570-96tests | Abbexa | 96 tests | EUR 990 |
Rat Gsk3a(Glycogen synthase kinase-3 alpha) ELISA Kit |
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ER0190 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.375 ng/ml |
Rat Gsk3a/ Glycogen synthase kinase-3 alpha ELISA Kit |
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E0420Ra | Sunlong | 1 Kit | EUR 675.6 |
Human GSK3A/ Glycogen synthase kinase-3 alpha ELISA Kit |
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E1064Hu | Sunlong | 1 Kit | EUR 644.4 |
Mouse Gsk3a/ Glycogen synthase kinase-3 alpha ELISA Kit |
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E0632Mo | Sunlong | 1 Kit | EUR 675.6 |
CLIA kit for Rat GSK3A (Glycogen Synthase Kinase 3 Alpha) |
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E-CL-R0301 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Rat GSK3A (Glycogen Synthase Kinase 3 Alpha) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Rat GSK3a (Glycogen Synthase Kinase 3 Alpha) |
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ELK1708 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Glycogen Synthase Kinase 3 Alpha from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
CLIA kit for Mouse GSK3a (Glycogen Synthase Kinase 3 Alpha) |
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E-CL-M0353 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Mouse GSK3a (Glycogen Synthase Kinase 3 Alpha) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human GSK3a (Glycogen Synthase Kinase 3 Alpha) |
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ELK1707 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Glycogen Synthase Kinase 3 Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Gsk3a ELISA Kit| Rat Glycogen synthase kinase-3 alpha ELISA Kit |
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EF017107 | Lifescience Market | 96 Tests | EUR 826.8 |
Human Glycogen Synthase Kinase 3 Alpha (GSK3Α) ELISA Kit |
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abx252586-96tests | Abbexa | 96 tests | EUR 764.4 |
Human GSK3α(Glycogen Synthase Kinase 3 Alpha) ELISA Kit |
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EH3185 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Mouse Gsk3α(Glycogen synthase kinase-3 alpha) ELISA Kit |
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EM0309 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 4.688pg/ml |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody |
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20-abx000728 | Abbexa |
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Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody |
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20-abx121488 | Abbexa |
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Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody |
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20-abx134166 | Abbexa |
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Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody |
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abx033884-400ul | Abbexa | 400 ul | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody |
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abx033884-80l | Abbexa | 80 µl | EUR 343.2 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody |
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20-abx445135 | Abbexa |
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ELISA kit for Rat GSK3? (Glycogen Synthase Kinase 3 Alpha) |
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E-EL-R0447 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Description: A sandwich ELISA kit for quantitative measurement of Rat GSK3? (Glycogen Synthase Kinase 3 Alpha) in samples from Serum, Plasma, Cell supernatant |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ALP) |
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abx445506-100ug | Abbexa | 100 ug | EUR 610.8 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233673-100ug | Abbexa | 100 ug | EUR 577.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233674-100ug | Abbexa | 100 ug | EUR 577.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233675-100ug | Abbexa | 100 ug | EUR 661.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx233676-100ug | Abbexa | 100 ug | EUR 610.8 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx224101-100ug | Abbexa | 100 ug | EUR 493.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx318062 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx172635 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx172636 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx328988 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx332790-100ul | Abbexa | 100 ul | EUR 510 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx037712-100ug | Abbexa | 100 ug | EUR 469.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx004714 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx001694 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx002277 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx112808 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx176674 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3b) Antibody |
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20-abx176675 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx125048 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx125336 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx125917 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx147405-100ug | Abbexa | 100 ug | EUR 526.8 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx010856-100ul | Abbexa | 100 ul | EUR 493.2 |
Glycogen Synthase Kinase 3 Beta (Gsk3b) Antibody |
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abx028354-400ul | Abbexa | 400 ul | EUR 627.6 |
Glycogen Synthase Kinase 3 Beta (Gsk3b) Antibody |
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abx028354-80l | Abbexa | 80 µl | EUR 343.2 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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abx332014-100ul | Abbexa | 100 ul | EUR 510 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx329709 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody |
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20-abx330217 | Abbexa |
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CLIA kit for Human GSK3? (Glycogen Synthase Kinase 3 Alpha) |
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E-CL-H1217 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Human GSK3? (Glycogen Synthase Kinase 3 Alpha) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Mouse GSK3? (Glycogen Synthase Kinase 3 Alpha) |
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E-EL-M0586 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Description: A sandwich ELISA kit for quantitative measurement of Mouse GSK3? (Glycogen Synthase Kinase 3 Alpha) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human GSK3? (Glycogen Synthase Kinase 3 Alpha) |
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E-EL-H2001 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Description: A sandwich ELISA kit for quantitative measurement of Human GSK3? (Glycogen Synthase Kinase 3 Alpha) in samples from Serum, Plasma, Cell supernatant |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (APC) |
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abx445507-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (RPE) |
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abx445513-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody (HRP) |
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20-abx304084 | Abbexa |
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Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody (FITC) |
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20-abx304085 | Abbexa |
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Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (PerCP) |
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abx445512-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase 3 Beta (GSK3B) Antibody (Biotin) |
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20-abx304086 | Abbexa |
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Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 390) |
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abx445498-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 488) |
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abx445499-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 565) |
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abx445500-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 594) |
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abx445501-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 633) |
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abx445502-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 655) |
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abx445503-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 680) |
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abx445504-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (ATTO 700) |
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abx445505-100ug | Abbexa | 100 ug | EUR 627.6 |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (Streptavidin) |
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abx445514-100ug | Abbexa | 100 ug | EUR 627.6 |
Rat Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E02G0264-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E02G0264-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E02G0264-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E07G0264-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Porcine Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E07G0264-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Porcine Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E07G0264-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Porcine Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E08G0264-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Canine Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E08G0264-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Canine Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Glycogen Synthase Kinase 3 Alpha ELISA kit |
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E08G0264-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Canine Glycogen Synthase Kinase 3 Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Glycogen Synthase Kinase-3 Beta (GSK3B) Antibody (PE/ATTO 594) |
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abx445511-100ug | Abbexa | 100 ug | EUR 644.4 |
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Constantly, long run therapy with glucosyl-ceramide synthase inhibitor PDMP was in a position to largely restore glycogen synthesis, glucose uptake and Glut-Four translocation upon Ins stimulation in HepG2 uncovered to PFOA. Our knowledge help a novel pathogenic mechanism linking publicity to PFOA to derangement of hepatocyte cell metabolism.
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