western blot sandwich, A capillary driven microfluidic chip for SERS based hCG detection

A capillary driven microfluidic chip for SERS based hCG detection

On this research, a capillary pushed microfluidic chip-based immunoassay was developed for the willpower of Human Chorionic Gonadotropin (hCG) protein, which is prohibited by the World Anti-Doping Company (WADA). Right here, we used antibody modified magnetic metallic natural framework nanoparticles (MMOFs) as a seize prob in urine pattern.
MMOF captured hCG was transferred in a capillary pushed microfluidic chip consisting of 4 chambers, and the interplay of MMOF with gold nanorods labelled with 5,5′-Dithiobis-(2-nitrobenzoic acid) (DTNB) as a Raman label was carried out within the capillary pushed microfluidic chip. The motion of MMOF by means of first chamber to the final chamber was achieved with a easy magnet.
Within the final chamber of capillary pushed microfluidic chip, SERS indicators of DTNB molecules from the sandwich advanced have been recorded utilizing a Raman spectrophotometer. The selectivity of the developed methodology was demonstrated by making use of the identical process for the detection of Human Luteinizing Hormone (hLH), Human Chorionic Gonadotropin Hormone (hGH) and Immunoglobulin G (IgG) protein.
The regression coefficient and restrict of detection obtained from the usual addition methodology have been discovered as 0,9985 and 0,61 IU/L, respectively. Moreover, the traditional ELISA methodology confirmed that the outcomes obtained by the offered methodology have been acceptable with the similarity of 97.9% when it comes to common restoration worth, for the detection of hCG in urine samples. The evaluation system developed for goal proteins might be an alternate approach comparable to Western Blot utilized in routine evaluation that’s costly and time consuming.

A easy unlabeled human chorionic gonadotropin biosensor based mostly on a peptide aptamer

As an important biochemical indicator within the fields of being pregnant and oncology, human chorionic gonadotropin (HCG) may be evaluated utilizing colloidal gold immunochromatographic paper and quantified utilizing a biochemical analyzer based mostly on the precept of the antibody sandwich methodology. In view of the inaccuracy of the previous and the complication of the latter, this research constructed an correct, delicate and easy unlabeled biosensor based mostly on peptide aptamer CGGGPPLRINRHILTR for HCG detection.
Molecular Working Surroundings (MOE) was used to simulate the aptamer and protein docking, and western blot (WB) was used to confirm the binding impact and ratio. The peptide aptamer was characterised and was then decreased with tris-(2-carboxyethyl)-phosphine hydrochloride (TCEP). After electrochemical deposition of chloroauric acid on the screen-printed electrode (SPE), the aptamer was self-assembled on the electrode floor below optimum situations.
The energetic website of the electrode floor was blocked with 6-mercapto-1-hexanol (MCH) and BSA. The electrochemical impedance spectrum (EIS) was used to quantify HCG within the matrix. Displaying a very good linear relationship within the vary of 5-1500 mIU mL-1, with a detection restrict of 1 mIU mL-1, the biosensor remained secure at room temperature for 14 days. Due to its small dimension, stability, sensitivity and accuracy, this biosensor has nice potential to grow to be a transportable diagnostic machine for HCG.

Augmentation of RBP4/STRA6 signaling results in insulin resistance and irritation and the believable therapeutic position of vildagliptin and metformin

A task of Retinol Binding Protein-4 (RBP4) in insulin resistance is extensively studied. Nonetheless, there’s paucity of data on its receptor viz., Stimulated by Retinoic Acid-6 (STRA6) with insulin resistance. To deal with this, we investigated the regulation of RBP4/STRA6 expression in 3T3-L1 adipocytes uncovered to glucolipotoxicity (GLT) and in visceral adipose tissue (VAT) from excessive fats eating regimen (HFD) fed insulin-resistant rats.
3T3-L1 adipocytes have been subjected to GLT and different experimental maneuvers with and with out vildagliptin or metformin. Actual-time PCR and westernblot experiments have been carried out to research RBP4, STRA6, PPARγ gene and protein expression.
Adipored staining and glucose uptake assay have been carried out to guage lipid and glucose metabolism. Oral glucose tolerance check (OGTT) and Insulin Tolerance Take a look at (ITT) have been carried out to find out the extent of insulin resistance in HFD fed male Wistar rats. Whole serum RBP4 was measured by quantitative sandwich enzyme-linked immunosorbent assay equipment.
Adipocytes below GLT exhibited considerably elevated RBP4/STRA6 expressions and decreased insulin sensitivity/glucose uptake. Vildagliptin and metformin not solely restored the above but in addition decreased the expression of IL-6, NFκB, SOCS-Three together with lipid accumulation.
Moreover, HFD fed rats exhibited considerably elevated serum ranges of RBP4 together with VAT expression of RBP4, STRA6, PPARγ, IL-6. These molecules have been considerably altered by the vildagliptin/ metformin remedy. We conclude that RBP4/STRA6 pathway is primarily concerned in mediating irritation and insulin resistance in adipocytes and visceral adipose tissues below glucolipotoxicity and in insulin resistant rats.

Structural and biochemical characterization of a novel thermophilic Coh01147 protease

Proteases play an important position in dwelling organisms and characterize one of many largest teams of business enzymes. The intention of this work was recombinant manufacturing and characterization of a newly recognized thermostable protease 1147 from thermophilum indigenous Cohnella sp. A01.
Phylogenetic tree evaluation confirmed that protease 1147 is intently associated to the cysteine proteases from DJ-1/ThiJ/PfpI superfamily, with the conserved catalytic tetrad. Structural prediction utilizing MODELLER 9v7 indicated that protease 1147 has an general α/β sandwich tertiary construction.
western blot sandwich, A capillary driven microfluidic chip for SERS based hCG detection
The gene of protease 1147 was cloned and expressed in Escherichia coli (E. coli) BL21. The recombinant protease 1147 appeared as a homogenous band of 18 kDa in SDS-PAGE, which was verified by western blot and zymography. The recombinant protein was purified with a yield of roughly 88% in a single step utilizing Ni-NTA affinity chromatography.
Moreover, a fast one-step thermal shock process was efficiently applied to purify the protein with a yield of 73%. Utilizing casein because the substrate, Km, and kcat, kcat/Km values of 13.72 mM, 3.143 × 10-3 (s-1), and 0.381 (M-1 S-1) have been obtained, respectively.
The utmost protease exercise was detected at pH = 7 and 60°C with the inactivation fee fixed (kin) of two.10 × 10-3 (m-1), and half-life (t1/2) of 330.07 min. Protease 1147 exhibited wonderful stability to natural solvent, metallic ions, and 1% SDS. The protease exercise was considerably enhanced by Tween 20 and Tween 80 and suppressed by cysteine protease particular inhibitors.

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40
EUR 16939

Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 356.00
  • EUR 523.00
  • 0.5 mg
  • 1 mg

Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 647
Description:
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ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 647
Description:
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ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 647
Description:
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 647
Description:
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HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 647
Description:
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RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 647
Description:
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TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 647
Description:
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ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 647
Description:
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GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 647
Description:
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PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 647
Description:
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AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 647
Description:
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GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 647
Description:
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PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 647
Description:
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LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 647
Description:
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HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 647
Description:
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PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 647
Description:
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ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 647
Description:
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OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 647
Description:
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ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 647
Description:
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SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 647
Description:
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SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 647
Description:
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RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 647
Description:
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ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 647
Description:
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A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 647
Description:
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  • Species reactivity: Human
  • Application:
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NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 647
Description:
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LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 647
Description:
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RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 647
Description:
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ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 647
Description:
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ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 647
Description:
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KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 647
Description:
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CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 647
Description:
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KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 647
Description:
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CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 647
Description:
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P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 647
Description:
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TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 647
Description:
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PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 647
Description:
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ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 647
Description:
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ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 647
Description:
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ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 647
Description:
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ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 647
Description:
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SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 647
Description:
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RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 647
Description:
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ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 647
Description:
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Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 647
Description:
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G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 647
Description:
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TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 647
Description:
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ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 647
Description:
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SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 647
Description:
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EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 647
Description:
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TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 647
Description:
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TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 647
Description:
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BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 647
Description:
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JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 647
Description:
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SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 647
Description:
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SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 647
Description:
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STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 647
Description:
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PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 647
Description:
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HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 647
Description:
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MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 647
Description:
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HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 647
Description:
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WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 647
Description:
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GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 647
Description:
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SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 647
Description:
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TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 647
Description:
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CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 647
Description:
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SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 647
Description:
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STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 647
Description:
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OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 647
Description:
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TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 647
Description:
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RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 647
Description:
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KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 647
Description:
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IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 647
Description:
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SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 647
Description:
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PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 647
Description:
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FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 647
Description:
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SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 647
Description:
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ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 647
Description:
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RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 647
Description:
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PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 647
Description:
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PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 647
Description:
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TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 647
Description:
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PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 647
Description:
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RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 647
Description:
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Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 392
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (TBS)

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 182

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 178

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 109
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 286

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 286

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 286

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 286

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 286

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50
EUR 191

Attoglow Western Blot Analysis Kit: Millennium Enhancer

K3171250-1 100 ml
EUR 163
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 143
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Western blot recycling stripping buffer, 50 ml (10X)

90101 50 ml
EUR 238

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Human Spleen Ferritin protein Western blot +ve control

FERT11-C 100 ul
EUR 286

Human Liver Ferritin protein Western blot +ve control

FERT12-C 100 ul
EUR 286

Horse Spleen Ferritin protein Western blot +ve control

FERT13-C 100 ul
EUR 286

Human C1 Esterase Inhibitor western blot +ve control

C1E11-C 100 ul
EUR 286

Purified Human C1q protein Western blot +ve control

C1Q11-C 100 ul
EUR 286

HRP Western Blot Anti-IgG Antibody Kit (OKRA00006)

OKRA00006 100 ug
EUR 205
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 286

Mouse Leptin protein Western blot +ve control # 1

LEP11-C 100 ul
EUR 286

Human Leptin protein Western blot +ve control # 2

LEP12-C 100 ul
EUR 286

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 286

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 286

Beta-Galactosidase (E. Coli) Protein for Western Blot

BGAL11-C 100 ul
EUR 286

Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
EUR 286

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 286

Single solution TMB substrate (membrane) solution for western blot

80110 100 mls
EUR 225

Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate

80200-Rb 1 kit
EUR 651

Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate

80201-Gt 1 kit
EUR 651

Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate

80202-Mo 1 kit
EUR 651

Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate

80203-Rt 1 kit
EUR 651

Western blot Kit for Chicken Primary Antibodies, Chemilum. Substrate

80204-Ch 1 kit
EUR 651

Western blot Kit for Monkey Primary Antibodies, Chemilum. Substrate

80205-Mk 1 kit
EUR 651

Western blot Kit for Human Primary Antibodies, Chemilum. Substrate

80207-Hu 1 kit
EUR 651

Western blot Kit for Sheep Primary Antibodies; Chemilum. Substrate

80208-Sh 1 kit
EUR 651

Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate

80209-Pg 1 kit
EUR 651

NuGlo ECL Substrate Kit for Western blot, 400 mls

80215 1 kit
EUR 408

WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection

ACT-WP
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 120

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 147

Zika Virus prM Protein (African) control for Western blot

ZPRM11-C 100 ul
EUR 286

HRP Western Blot Anti-Rabbit IgG Antibody Kit (OKRA00007)

OKRA00007 100 ug
EUR 205
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Biotin Western Blot Anti-IgG Antibody MaxTag Kit (OKRA00023)

OKRA00023 1 ml
EUR 283
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Prealbumin (Transeryhtin/TTR) Human, protein control for Western blot

PALB11-C 100 ul
EUR 286

Recombinant Mycoplasma Pulmonis (Mp) protein control for Western Blot

MPUL11-C 100 ul
EUR 445

Human hemoglobin (non-glycated) protein control for western blot

HEMG13-C 100 ul
EUR 286

Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control

COX11-C 100 ul
EUR 286

Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control

COX21-C 100 ul
EUR 286

Camelpox virus H3L/p35 protein control for western blot

CPOX11-C 100 ul
EUR 286

Human Liver Cathepsin B protein control for Western blot

CTHB11-C 100 ul
EUR 286

Human Liver Cathepsin D protein control for Western blot

CTHD11-C 100 ul
EUR 286

Human Liver Cathepsin G protein control for Western blot

CTHG11-C 100 ul
EUR 286

Human Liver Cathepsin H protein control for Western blot

CTHH11-C 100 ul
EUR 286

Human Liver Cathepsin L protein control for Western blot

CTHL11-C 100 ul
EUR 286

Human Adipsin/Factor D protein Western blot +ve control

ADN12-C 100 ul
EUR 286

Purified Mouse serum albumin protein control for Western blot

ALB11-C 100 ul
EUR 286

Purified Human serum albumin protein control for Western blot

ALBH13-C 100 ul
EUR 286

Purified rat serum albumin protein control for Western blot

ALBR12-C 100 ul
EUR 286

Purified Sheep serum albumin protein control for Western blot

ALBSH20-C 100 ul
EUR 286

Recombinant human BAFF-R protein control for Western blot

BAFFR11-C 100 ul
EUR 286

Human recombinant, purified BMP2 protein Western blot +ve control

BMP21-C 100 ul
EUR 286

Human recombinant, purified BMP3 protein Western blot +ve control

BMP31-C 100 ul
EUR 286

Human recombinant, purified BMP4 protein Western blot +ve control

BMP41-C 100 ul
EUR 286

Human recombinant, purified BMP5 protein Western blot +ve control

BMP51-C 100 ul
EUR 286

Human recombinant, purified BMP6 protein Western blot +ve control

BMP61-C 100 ul
EUR 286
Docking outcomes and molecular dynamics (MD) simulation revealed that Tween 20 interacted with protease 1147 through hydrogen bonds and made the construction extra secure. CD and fluorescence spectra indicated structural adjustments happening at 100°C, very primary and acidic pH, and within the presence of Tween 20. These properties make this newly characterised protease a possible candidate for varied biotechnological functions.

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