Tag: pglo plasmid
Antibody-Free Mass Spectrometry Identification of Vascular Integrity Markers in Major Trauma
Antibody mediated methods for protein biomarker detection are widespread, however might restrict discovery. We hypothesized that the usage of antibody-free proteomics is possible for detecting protein biomarkers in plasma of sufferers sustaining main trauma. A subset of topics with main trauma from a potential observational trial had been analyzed. Sufferers had been assigned to one in all…Read More
glucose uptake assay protocol, Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
Fatty acid β-oxidation is a key metabolic pathway to fulfill the power calls for of the liver and supply substrates and cofactors for added processes, akin to ketogenesis and gluconeogenesis, that are important to take care of whole-body glucose homeostasis and help extra-hepatic organ perform within the fasted state. Fatty acid β-oxidation happens inside the mitochondria and…Read More
Tyrosine hydroxylase: purification from PC-12 cells, characterization and production of antibodies.
Tyrosine hydroxylase has been purified to homogeneity from cultured PC-12 cells. The protein migrates as a single band with a molecular weight of 60,000 on sodium dodecyl sulfate polyacrylamide electrophoresis. Two-dimensional electrophoresis of the pure enzyme resolves three spots (every with molecular weights of 60,000) with isoelectric factors of 5.4, 5.Eight and 5.9. This cost…Read More