glucose uptake assay protocol, Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Fatty acid β-oxidation is a key metabolic pathway to fulfill the power calls for of the liver and supply substrates and cofactors for added processes, akin to ketogenesis and gluconeogenesis, that are important to take care of whole-body glucose homeostasis and help extra-hepatic organ perform within the fasted state. Fatty acid β-oxidation happens inside the mitochondria and…

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Tyrosine hydroxylase: purification from PC-12 cells, characterization and production of antibodies.

Tyrosine hydroxylase: purification from PC-12 cells, characterization and production of antibodies.

Tyrosine hydroxylase has been purified to homogeneity from cultured PC-12 cells. The protein migrates as a single band with a molecular weight of 60,000 on sodium dodecyl sulfate polyacrylamide electrophoresis. Two-dimensional electrophoresis of the pure enzyme resolves three spots (every with molecular weights of 60,000) with isoelectric factors of 5.4, 5.Eight and 5.9. This cost…

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Mycoplasma-Off Surface, 1L

Mycoplasma Off is used for cleaning and disinfecting all types of laboratory surfaces and devices, including safety cabinets, incubators, workbenches, sample storage boxes, and liquid nitrogen containers. Quickly effective against mycoplasma contamination, Mycoplasma Off ™ has broad antimicrobial activity against several other contaminants. Profits Convenient and Fast Mycoplasma Off ™ is a ready-to-use solution supplied…

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