
Validation of a monoclonal antibody directed against the human sphingosine 1-phosphate receptor type 1
Lieven
- 0
The sphingosine 1-phosphate receptor kind 1 (S1PR1) has a number of vital features, together with stabilizing endothelial barrier and sustaining lymphocyte circulation. These features are critically depending on the regulation of S1PR1 cell floor expression. Presently obtainable antibodies in opposition to human S1PR1 are usually not in a position to decide up cell floor expression on residing cells by movement cytometry as a consequence of intracellular epitopes or unspecific binding.
Right here we describe the era of a mouse monoclonal antibody particular for the N-terminal area of human S1PR1. It has an immunoglobulin M (IgM) kappa isotype and detects cell floor expression of recombinant human S1PR1 on overexpressing cells. As a consequence of unspecific intracellular cell staining, it can’t be used for staining of useless cells and tissue slides or in microscopic analyses.
It’s also not appropriate for Western blot evaluation and immunoprecipitation. Nonetheless, the antibody can stain for endogenous S1PR1 on human endothelial cell traces and first human umbilical vein endothelial cells (HUVEC). Incubation of those cells with numerous S1PR1 agonists revealed potent S1PR1 internalization, which was not the case with the particular antagonist W146.
Surprisingly, human T and B cells remoted from blood and palatine tonsils didn’t present particular staining, demonstrating considerably decrease endogenous S1PR1 floor expression on lymphocytes than on endothelial cells.
Sphingosine-1-phosphate receptor modulator FTY720 attenuates experimental myeloperoxidase-ANCA vasculitis in a T cell-dependent method
Inflammatory Circumstances Disrupt Constitutive Endothelial Cell Barrier Stabilization by Assuaging Autonomous Secretion of Sphingosine 1-Phosphate.
The mechanism of the premetastatic area of interest facilitating colorectal most cancers liver metastasis generated from myeloid-derived suppressor cells induced by the S1PR1-STAT3 signaling pathway.

Sphingosine 1-phosphate however not Fingolimod protects neurons in opposition to excitotoxic cell dying by inducing neurotrophic gene expression in astrocytes.
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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DLR-S1PR1-Mu-48T | DL Develop | 48T | EUR 632.4 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) in samples from tissue homogenates, cell lysates or other biological fluids. |
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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DLR-S1PR1-Mu-96T | DL Develop | 96T | EUR 825.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RD-S1PR1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 625.2 |
Human Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RD-S1PR1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 867.6 |
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RD-S1PR1-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 639.6 |
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RD-S1PR1-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 888 |
Human Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RDR-S1PR1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 652.8 |
Human Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RDR-S1PR1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 907.2 |
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RDR-S1PR1-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 668.4 |
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit |
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RDR-S1PR1-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 928.8 |
S1PR1 antibody |
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70R-20069 | Fitzgerald | 50 ul | EUR 522 |
Description: Rabbit polyclonal S1PR1 antibody |
S1PR1 Antibody |
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37288-100ul | SAB | 100ul | EUR 302.4 |
S1PR1 Antibody |
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1-CSB-PA002247 | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
S1PR1 Antibody |
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1-CSB-PA193689 | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
S1PR1 Antibody |
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1-CSB-PA241754 | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
S1PR1 Antibody |
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1-CSB-PA007898 | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000 |
S1PR1 Antibody |
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1-CSB-PA020650GA01HU | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
S1PR1 Antibody |
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1-CSB-PA020650LA01HU | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
S1pr1 Antibody |
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F52393-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 379 |
S1PR1 Conjugated Antibody |
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C37288 | SAB | 100ul | EUR 476.4 |
Anti-S1PR1 antibody |
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STJ114801 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. |
S1PR1 Polyclonal Antibody |
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A70547-050 | EpiGentek | 50 ul | EUR 302.5 |
S1PR1 Polyclonal Antibody |
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A70547-100 | EpiGentek | 100 ul | EUR 423.5 |
S1PR1 Polyclonal Antibody |
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A50464-020 | EpiGentek | 20 ul | Ask for price |
S1PR1 Polyclonal Antibody |
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A50464-050 | EpiGentek | 50 ul | Ask for price |
S1PR1 Polyclonal Antibody |
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A50464-100 | EpiGentek | 100 ul | EUR 423.5 |
S1PR1 Polyclonal Antibody |
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A50464 | EpiGentek |
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S1PR1 Polyclonal Antibody |
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A70547 | EpiGentek |
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S1pr1 Antibody (Zebrafish) |
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F52393-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
S1PR1 siRNA |
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20-abx904763 | Abbexa |
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S1PR1 siRNA |
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20-abx932372 | Abbexa |
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S1PR1 siRNA |
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20-abx932373 | Abbexa |
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Phospho-S1PR1 (T236) Antibody |
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1-CSB-PA007881 | Cusabio |
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Description: A polyclonal antibody against Phospho-S1PR1 (T236). Recognizes Phospho-S1PR1 (T236) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;IF:1/200-1/1000.ELISA:1/10000 |
S1PR1 Antibody, HRP conjugated |
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1-CSB-PA020650LB01HU | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
S1PR1 Antibody, FITC conjugated |
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1-CSB-PA020650LC01HU | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
S1PR1 Antibody, Biotin conjugated |
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1-CSB-PA020650LD01HU | Cusabio |
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Description: A polyclonal antibody against S1PR1. Recognizes S1PR1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Anti-S1PR1/EDG1 antibody |
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PAab07568 | Lifescience Market | 100 ug | EUR 463.2 |
anti- S1PR1/EDG1 antibody |
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FNab07568 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against S1PR1/EDG1 |
S1PR1 Rabbit pAb |
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A12935-100ul | Abclonal | 100 ul | EUR 369.6 |
S1PR1 Rabbit pAb |
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A12935-200ul | Abclonal | 200 ul | EUR 550.8 |
S1PR1 Rabbit pAb |
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A12935-20ul | Abclonal | 20 ul | EUR 219.6 |
S1PR1 Rabbit pAb |
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A12935-50ul | Abclonal | 50 ul | EUR 267.6 |
S1PR1 cloning plasmid |
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CSB-CL020650HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the S1PR1 gene. |
S1PR1-MO-1 |
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HY-U00366 | MedChemExpress | 5mg | EUR 5415.6 |
Polyclonal S1PR1 / EDG1 / S1P1 Antibody |
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APR02818G | Leading Biology | 0.05ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human S1PR1 / EDG1 / S1P1 . This antibody is tested and proven to work in the following applications: |
S1PR1 Polyclonal Antibody, HRP Conjugated |
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A50465-050 | EpiGentek | 50 ul | EUR 302.5 |